Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023418 (leukemia)
 93,477 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Dystroglycan is a laminin binding protein, which provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix. It is also involved in the organization of basement membranes. So far the genomic organization of the dystroglycan gene DAG1 has not been completely investigated. Here we report the cloning and sequencing of 162 kb of dog genomic DNA containing the complete approximately 71-kb canine DAG1 gene, which consists of three exons, with the translation start codon located in exon 2. Its 2679-nucleotide ORF encodes a polypeptide of 892 amino acids, which is highly similar to human, rabbit, and bovine orthologs. To further characterize the dog DAG1 gene we determined the transcription start site and several naturally occurring polymorphisms, which partially result in amino acid substitutions of the dystroglycan protein. The dog DAG1 gene was assigned to chromosome 20q15.1-q15.2 by FISH analysis. The analysis of the entire reported sequence revealed that the genes for aminomethyltransferase (AMT), bassoon (BSN), TCTA (T-cell leukemia translocation-associated) gene, and an as yet uncharacterized protein are located very close to the DAG1 gene. Therefore, this study defines a novel syntenic region among dog chromosome 20q15, human chromosome 3p21, and murine chromosome 9F.
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PMID:Genomic organization of the dog dystroglycan gene DAG1 locus on chromosome 20q15.1-q15.2. 1072 May 70

Analysis of genomic sequence from canine chromosome 20q15.1-->q15.2 revealed the presence of two closely linked genes. The two genes represent the corresponding canine orthologs of human aminomethyltransferase (AMT) and the human T-cell leukemia translocation associated (TCTA) gene. Aminomethyltransferase or glycine cleavage system T-protein is an important enzyme in glycine metabolism. The reported canine AMT gene spans 5 kb and consists of nine exons. It encodes a protein of 403 amino acids with 88% identity to human aminomethyltransferase. Human TCTA is located on 3p21 near the breakpoint of a t(1;3) translocation observed in some cancer cell lines. The 4-kb canine TCTA gene consists of three exons and probably represents a pseudogene. It is located adjacent to AMT and very close to DAG1 and BSN.
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PMID:Genomic structures and sequences of two closely linked genes (AMT, TCTA) on dog chromosome 20q15.1-->q15.2. 1089 47