Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023418 (leukemia)
 93,477 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Minute virus of mice (MVM), a non-defective parvovirus, has been shown to infect cultures of non-pluripotent differentiated teratocarcinoma-derived cells, but pluripotent (and "nullipotent") embryonal carcinoma cells derived from the same teratocarcinoma resist MVN infection. Somatic cell hybrids between an embryonal carcinoma line and Friend erythroblastic leukemia cells are also resistant to MVM, even though Friend cells are susceptible. Among three blastocyst-derived lines tested, only one, a parietal yolk sac cell line, resists MVM infection. These results suggest that teratocarcinoma cultures may provide useful systems in which to study the cellular factors which mediate susceptibility to this teratogenic and oncolytic virus.
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PMID:Embryonal carcinoma cells (and their somatic cell hybrids) are resistant to infection by the murine parvovirus MVM, which does infect other teratocarcinoma-derived cell lines. 55 90

The nonpathogenic human defective parvovirus adeno-associated virus (AAV) type 2 induced differentiation-associated antigens in cells of the human leukemia cell line HL60 (CD 67), as well as in two different lines of immortalized human keratinocytes, HaCaT and HPK Ia cells (involucrin and cytokeratin 10). Simultaneously, expression of the c-myc and c-myb oncogenes and the retinoblastoma gene was down regulated whereas c-fos expression increased in infected cells. These data point to the potential of AAV to induce functions related to the differentiation pathway in different types of human cells. This phenomenon may be involved in the reported oncosuppressive properties of AAV infections.
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PMID:Induction of differentiation-associated changes in established human cells by infection with adeno-associated virus type 2. 131

The clinical and pathological findings in 19 ferrets (Mustela putorius furo) with malignant lymphoma are reviewed. Peripubescent ferrets had rapidly progressive stage IV high grade immunoblastic or small non-cleaved cell lymphoma. Adult ferrets had stage II or IV low grade diffuse small lymphocytic (DSL) lymphoma, stage IV high grade small non-cleaved cell lymphoma, or stage IV high grade immunoblastic polymorphous (IBP) lymphoma. Three ferrets had concurrent IBP and DSL lymphoma involving different organs. The IBP admixture of immunoblasts, large atypical lymphocytes, Reed-Sternberg-like cells, lymphoblasts and small lymphocytes has been associated with certain retrovirally associated lymphomas and nodal hyperplasias in man, non-human primates and cats. Aleutian disease, a parvovirus-induced lymphoproliferative disease, also involves clinical and histological features similar to certain lymphomas in ferrets. Seven ferrets tested were seronegative for feline leukaemia virus antigen. Only one of eight ferrets was positive for Aleutian parvovirus antibody. The clinical and pathological findings are suggestive of a viral aetiology for certain lymphomas in ferrets.
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PMID:Malignant lymphoma in ferrets: clinical and pathological findings in 19 cases. 155 56

The pathogenic human parvovirus B19 has extreme tropism for human erythroid progenitor cells and has resisted cultivation in conventional cell lines. We report first propagation of this virus in an erythropoietin-dependent strain of a megakaryoblastic leukemia cell line called UT-7. Virus protein was present in about 5% of cells after 1 week of culture. Appropriate ratios of major and minor capsid proteins were determined by immunoblot, and newly synthesized capsid protein was detected by immunoprecipitation of radioactively labeled cell lysates. High molecular weight monomer and dimer intermediates were detected by Southern analysis, indicating active viral replication. Approximately 1,000 genome copies were present per infected cell, and at the optimal multiplicity of infection 20- to 50-fold more virus was produced than inoculated. Virus propagation only occurred in UT-7 cells that were adapted to growth in erythropoietin; virus signal was not detected in UT-7 cells adapted for growth in granulocyte-macrophage colony-stimulating factor or interleukin-3, even with exposure to erythropoietin for several days. Infectious virus was detected in cultures as long as 3 months after inoculation. Despite persistence, there was no evidence of viral integration on Southern analysis. This cell line may prove useful for the production of infectious virus and in the analysis of B19 parvovirus persistence, cytotoxicity, and permissivity.
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PMID:First continuous propagation of B19 parvovirus in a cell line. 172 7

Biotechnological methods offer promising approaches for improved diagnostic and prophylactic purposes. The following biotechnological techniques are used in the Institute of Virology at the Hanover Veterinary School:--Production of monoclonal antibodies directed against viral and bacteria-specific antigens such as bovine virus diarrhoea virus, classical swine fever (hog cholera) virus, feline leukaemia virus, animal parvoviruses, Alphavirus, Brucella and Francisella--Establishment of improved and sensitive diagnostic enzyme immunoassays (ELISA) using monoclonal antibodies--Molecular cloning and sequencing of classical swine fever virus RNA and parvovirus DNA--Development of diagnostic hybridisation techniques (dot, slot, Southern and Northern blot, in situ, oligonucleotides)--Detection of viral genomes in tissues of infected animals--Development of synthetic oligopeptides as diagnostic antigens and as potential immunogens for vaccines. Currently available techniques used in basic research (e.g. pathogenesis studies) will be tested for their application in routine diagnosis of viral diseases, e.g. by molecular hybridisation. Some techniques need to be simplified (e.g. RNA extraction procedures) and, particularly, alternative labelling schedules must be developed (e.g. biotin or sulfone labelling instead of radionuclides).
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PMID:Use of biotechnical methods in veterinary medicine. 196 25

Opportunistic infections such as Pneumocystis carinii pneumonia are well-recognised in patients with the acquired immune deficiency syndrome (AIDS). Anaemia due to a variety of causes also occurs in AIDS. Persistent infection with parvovirus (B19) causing severe anaemia has been reported in patients with leukaemia and congenital immunodeficiency. A case is now reported of parvovirus infection and anaemia, in an adult with AIDS, which responded dramatically to immunoglobulin therapy.
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PMID:Parvovirus infection and anaemia in a patient with AIDS: case report. 216 Apr 24

Two children developed severe anemia with reticulocytopenia while on maintenance chemotherapy for acute lymphoblastic leukemia. Bone marrow examination revealed marked erythroid hypoplasia and giant megaloblasts without evidence of relapse. One patient had evidence of B19 Parvoviremia at the time of severe anemia but failed to produce anti B19 antibodies. Despite the failure to mount an antibody response, the patient had no recurrence of viremia or anemia during the two years of follow-up after the infection. The other patient had no evidence of viremia or elevated anti-B19 antibodies at the time of anemia. However, when his serum was tested 16 months after the episode of anemia, he had elevated IgG and IgM antibodies against B19 parvovirus. The patient did not experience recurrent viremia or anemia over a two year period. Thus, patients with leukemia are at risk to develop severe anemia when infected with B19 parvovirus.
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PMID:Severe anemia due to B19 parvovirus infection in children with acute leukemia in remission. 216 47

Persisting DNA of parvovirus H-1 could be demonstrated in cells of two human lymphoma cell lines, the Burkitt lymphoma cell line BL2 and the T-cell leukemia cell line Jurkat which survived infection with parvovirus H-1. Persistence of H-1 DNA rendered the cells resistant to a second H-1 infection. This resistance to H-1 superinfection persisted even after loss of H-1 DNA occurring after approximately 150-200 cell generations. Resistance to H-1 superinfection was accompanied by reduced uptake of infectious particles and by a block of H-1 DNA replication. This suggests that persistent H-1 infection leads to modifications of cellular functions involved in the permissivity for H-1.
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PMID:Persistence of parvovirus H-1 DNA in human B- and T-lymphoma cells. 238 60

Syngeneic monoclonal anti-idiotypic antibodies were raised against idiotopes on neutralizing monoclonal antibodies with specificity for feline leukemia virus and canine parvovirus. The anti-idiotypic antibodies were shown to recognize paratope-related private idiotopes. Mice were injected with the monoclonal anti-idiotypic antibodies and the sera of these mice were screened for antiviral reactivities. Antibodies to both feline leukemia and canine parvovirus could be induced as determined by ELISA. These results suggest that anti-idiotypic antibodies which detect private idiotopes and thus do not represent internal images of viral antigens may be considered as candidates for the induction of antiviral immunity.
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PMID:Induction of anti-viral immune response by immunization with monoclonal anti-idiotype antibodies directed to private idiotopes. 248 29

The efficacy of two heating cycles (90 sec at 103 degrees C and 10 hr at 65 degrees C) used during manufacture of a plasma-derived hepatitis-B vaccine was validated for the inactivation of 12 virus families. A period of 15 min warming up to 65 degrees C had already completely inactivated representatives of nine virus families, ie, poxvirus (vaccinia), picornavirus (encephalomyocarditis virus), togavirus (sindbis virus), coronavirus (mouse hepatitis virus), orthomyxovirus (influenza virus), rhabdovirus (vesicular stomatitis virus), herpes virus (cytomegalovirus), lentivirus (human immunodeficiency virus), and retrovirus (murine leukemia virus). After prolonged heating at 65 degrees C or heating for 90 sec at 103 degrees C, parvovirus (canine parvovirus) and the phage phiX174 were also completely inactivated. Papovavirus represented by simian virus 40 (SV-40) was the most heat-resistant virus evaluated. The infectivity of SV-40 was reduced by 10(4) Tissue Culture Infectious Doses (TCID50) per ml after 90 sec at 103 degrees C, but a marginal residual activity (less than 1.5 TCID50 per ml) was observed. Subsequent pasteurization for 10 h at 65 degrees C did not further reduce the infectivity of SV-40. This study shows that the two heat-inactivation steps used during the production of this vaccine kill a wide variety of viruses that might be present in human blood.
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PMID:Inactivation of 12 viruses by heating steps applied during manufacture of a hepatitis B vaccine. 282 25


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