Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023241 (Legionella)
 6,990 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We studied the lipopolysaccharide (LPS) of Legionella pneumophila and six other Legionella species to determine whether strain differences were apparent. The LPS was purified by a cold ethanol extraction procedure, and total carbohydrates represented 10 to 20% of LPS weight. 2-keto-3-deoxyoctonate represented 1 to 13% of the total carbohydrate present in the LPS. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all strains except L. dumoffi showed smooth-type LPS with multiple high-molecular-weight complexes. Proteinase K-treated, whole-cell lysates showed profiles similar to those of purified LPS. Each serogroup of L. pneumophila and each Legionella species had a distinct sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile. L. pneumophila lipid A is antigenically related to the lipid A of Enterobacteriaceae. In immunoblot assays with the LPS of L. pneumophila serogroups 1 to 6 as antigens, serogroup-specific immune monkey sera recognized homologous purified LPS, but not the LPS of the five heterologous serogroups. These studies indicate that LPS composition may be a determinant of serogroup specificity as defined by the immunofluorescence-based serogrouping schema for L. pneumophila and other Legionella species.
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PMID:Serogroup specificity of Legionella pneumophila is related to lipopolysaccharide characteristics. 241 53

Proteinases produced by various Legionella species were studied by means of caseinate precipitation. Proteinase production was particularly high in strains of Legionella pneumophila (serogroups 1-6), much lower in other Legionella species, and absent in Legionella micdadei strain Tatlock. Immunoglobulins against proteinases of Legionella pneumophila (serogroup 1, strain Philadelphia 1) inhibited the proteinase activity of all strains of Legionella pneumophila, Legionella bozemanii, Legionella dumoffi and Legionella gormanii. There was no cross-reactivity between these antibodies and proteinases from bacteria belonging to other genera. Antibodies against Legionella pneumophila proteinases were not found in human convalescent sera. The proteinases of Legionella species could possibly be associated with pathogenicity factors.
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PMID:Occurrence and immunogenicity of proteinases from Legionella species. 618 21