Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023241 (Legionella)
 6,990 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In the 1st communication it was possible to show that some hoses and insufficiently cleaned high grade steel pipe can produce a microbial growth. The growth-promoting effect of materials in the water distribution system for Legionella pneumophila has been discussed before. In this investigation it was tested how L. pneumophila behaves in pipes and hoses with narrow diameter, at temperatures from 35 degrees C to 40 degrees C and over a time of half a year. L. pneumophila could be found in high numbers in the water from PVC, PE, PTFE, rubber and silicon hoses all over the time and regularly in low numbers or occasionally in glass, high-grade steel pipes and PA hose. L. pneumophila could be found only for the first 4 weeks in the copper pipe.
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PMID:[Microbial contamination of water by pipe and tubing material. 2. Growth of Legionella pneumophila]. 314 May 36

The design, development, and application of a fluorescent fiber-optic immunosensor (FFOI) procedure for the detection of antibody/antigen binding within the near-infrared (NIR) spectral region is reported. The technique was developed through the combined use of fiber-optics, semiconductor laser excitation, fluorescence detection, NIR dye, and immunochemical techniques. The antibody is immobilized on the FFOI's sensing tip and utilized as a recognition component for trace amounts of specific antigen. The FFOI is constructed to utilize antibody sandwich technique. Three individual immunoassays are reported. The first two assays utilize the FFOI and NN382, a commercial NIR dye, for the detection of human immunoglobulin G (IgG). In these assays, goat anti-human IgG antibody (GAHG) is immobilized on the sensitive terminal of the FFOI followed by the exposure of the antibody-coated terminal to human IgG. The probe is then introduced to GAHG labeled with NN382, generating a signal. The third assay utilizes the FFOI for the detection of trace amounts of Legionella pneumophila serogroup 1 (LPS1). In this assay, rabbit anti-LPS1 antibody is immobilized on the sensitive terminal of the FFOI followed by exposure to LPS1. The antigen-coated probe is then treated with monoclonal anti-LPS1 antibody followed by incubation with GAHG labeled with NN382. The assays are optimized to detect the corresponding antigen via the NIR-FFOI. Typical measurements are performed in 10-15 min. A 780-nm semiconductor laser provides the excitation of the immune complex and the resulting emission is detected by a 820-nm silicon photodiode detector. The intensity of the resulting fluorescence is directly proportional to the concentration of the antigen. Solutions of IgG and LPS1 with concentrations as low as 10(-11) M and 0.5 ng/ml, respectively, have been detected with a minimum interference.
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PMID:Detection of biomolecules in the near-infrared spectral region via a fiber-optic immunosensor. 1041 Sep 77