Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023241 (Legionella)
 6,990 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

It has been shown that electropherograms of DNA sequences can be modeled with hidden Markov models. Basecalling, the procedure that determines the sequence of bases from the given eletropherogram, can then be performed using the Viterbi algorithm. A training step is required prior to basecalling in order to estimate the HMM parameters. In this paper, we propose a Bayesian approach which employs the Markov chain Monte Carlo (MCMC) method to perform basecalling. Such an approach not only allows one to naturally encode the prior biological knowledge into the basecalling algorithm, it also exploits both the training data and the basecalling data in estimating the HMM parameters, leading to more accurate estimates. Using the recently sequenced genome of the organism Legionella pneumophila we show that the MCMC basecaller outperforms the state-of-the-art basecalling algorithm in terms of total errors while requiring much less training than other proposed statistical basecallers.
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PMID:Bayesian basecalling for DNA sequence analysis using hidden Markov models. 1766 62

The estates team at St George's Hospital in south London say the deployment of a web-based software system which verifies that 'responsible' staff across the site have regularly flushed low-use water outlets to maintain flow, and thus prevent potentially dangerous waterborne bacteria building up in pipework, has 'very substantially' increased compliance with approved flushing practice--as set out in the HSE's L8 Approved Code of Practice on controlling Legionella bacteria in water systems, and the new HTM 04-01, Safe Water in Healthcare Premises. As HEJ editor, Jonathan Baillie, reports, the hospital's use of Digital Missives' L8guard software system has also saved considerable staff time, eliminated the need to manually input data from thousands of paper flushing return forms, and enabled the Estates team to easily identify departments not undertaking regular flushing.
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PMID:Flushing compliance enhanced at St George's. 2949 11