Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0023241 (
Legionella
)
6,990
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Francisella tularensis
is a highly infectious, intracellular bacterium and it is the causative agent of tularemia. The bacterium has been isolated from more than 250 species, including protozoa. Previous studies have shown that the growth of
Legionella
pneumophila
within the amoeba results in a dramatic increase in the resistance to disinfectants. Since
Francisella
persists in the environment for years, this study investigates whether
Acanthamoeba castellanii
-grown
F. novicida
exhibits an alteration in the resistance to disinfectants. The disinfectants used are didecyldimethylammonium chloride (DDAC) combined with isopropyl alcohol (D1), benzalkonium chloride combined with DDAC and
formic acid
(D2), and polyhexamethylene biguanide (PHMB, D3). The effect of disinfectants on the bacterial viability is determined by a colony-forming unit (CFU), by transmission electron microscopy (TEM), by fluorescence microscopy, and the damage of the bacterial membrane. Our data has shown that only a one-log
10
loss in bacterial viability is exhibited upon treatment of agar-grown
Francisella
, while in amoeba-grown
Francisella
there was a three-log
10
difference with D3. The D1 disinfectant sterilized the bacteria within 10 s. The treatment of agar-grown
F. novicida
with D2 reduces bacterial viability by seven-log
10
within 10 s and 15 min, respectively. Surprisingly, the treatment of amoeba-grown
F. novicida
with D2 results in a total loss of bacterial viability. In conclusion,
A. castellanii-
grown
F. novicida
is more susceptible to many disinfectants.
...
PMID:Increased Sensitivity of Amoeba-Grown
Francisella
Species to Disinfectants. 3282 90
