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Query: UMLS:C0023241 (Legionella)
 6,990 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Legionella pneumophila serogroup 1 was isolated from antemortem aerobic blood culture bottles not supplemented with L-cysteine or ferric pyrophosphate and from the postmortem lung tissue of a 72-year-old man. We recommend that aerobic blood culture bottles (Johnston Laboratories, Cockeysville, Md.) of BACTEC be subcultured to an agar that supports the growth of L. pneumophila when growth indexes range from 30 to 60 but fail to increase further.
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PMID:Isolation of Legionella pneumophila serogroup 1 from blood with nonsupplemented blood culture bottles. 633 43

Legionella pneumophila serogroup 1 was observed to satellite around colonies of Flavobacterium breve on an L-cysteine-deficient medium which did not support growth of legionellae. Both isolates were recovered from the hot water tanks of hospitals. Ferric PPi stimulated satellite growth between 0.01 and 0.1%.
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PMID:Satellite growth of Legionella pneumophila with an environmental isolate of Flavobacterium breve. 666 Aug 82

From 15 to 21 August 1981, Pontiac fever affected 317 automobile assembly plant workers. Results of serologic tests were negative for Mycoplasma, Chlamydia, respiratory tract viruses, and previously described legionellae. A gram-negative, rod-shaped organism (WO-44C) that did not grow on blood agar, required L-cysteine for growth, and contained large amounts of branched-chain fatty acids was isolated from a water-based coolant. The organism did not react with antisera against other legionellae, and on DNA hybridization the organism was less than 10% related to other Legionella species. Geometric mean titers found by indirect fluorescent antibody testing to WO-44C were significantly higher in ill employees than in controls (p = 0.0001). Attack rates by department decreased linearly with the department's distance from the implicated coolant system. The etiologic agent apparently was a new Legionella species; we propose the name Legionella feeleii species nova (AATC 35072). This is the first outbreak of nonpneumonic legionellosis in which the etiologic agent is not L. pneumophila, serogroup 1.
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PMID:A new Legionella species, Legionella feeleii species nova, causes Pontiac fever in an automobile plant. 669 54

Six strains of a new species, Legionella sainthelensi, were isolated from freshwater in areas affected by the volcanic eruptions of Mt. St. Helens in the state of Washington. Strains of L. sainthelensi are culturally and biochemically similar to other legionellae. They grow on buffered charcoal yeast agar but not on media that lack cysteine. They are gram-negative, nonsporeforming, motile rods that are positive in reactions for catalase, oxidase, gelatin liquefaction, and beta-lactamase. They are negative in reactions for urease, hydrolysis of hippurate, reduction of nitrates, fermentation of glucose, and blue-white autofluorescence. Their cell wall fatty acid composition is qualitatively similar to those of other legionellae, with 50 to 62% branched-chain fatty acids. They contain the isobranched-chain 14- and 16-carbon acids and anteisobranched-chain 15- and 17-carbon acids and relatively large amounts of straight-chain 16-carbon acid. All strains of L. sainthelensi contain approximately equal amounts of ubiquinones Q9, Q10, Q11, and Q12, a pattern similar to those of Legionella bozemanii, Legionella dumoffi, and Legionella longbeachae. Serological cross-reactions were observed between L. sainthelensi, both serogroups of L. longbeachae, and Legionella oakridgensis. Three strains of L. sainthelensi were greater than 90% related by DNA hybridization. The type strain of L. sainthelensi, Mt. St. Helens 4, was 36% related to the type strain of L. longbeachae and 3 to 14% related to the other nine described Legionella species.
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PMID:Legionella sainthelensi: a new species of Legionella isolated from water near Mt. St. Helens. 671 10

The amino acids required for growth and as energy sources by 10 strains of Legionella pneumophila were determined by using a chemically defined medium. All strains required arginine, cysteine, isoleucine, leucine, threonine, valine, methionine, and phenylalanine or tyrosine. Most strains (7 of 10) required serine, and two strains had to be supplied proline before growth could be established. All 10 strains used serine and, to a lesser extent, threonine as the sole sources of carbon and energy. The Y serine calculated was 94.9 +/- 8.5 g (dry weight) of cells/mol of serine. Assuming that the value of Y adenosine 5'-triphosphate is 10.5, these results indicate that oxidative catabolism of 1 mol of serine yielded approximately 9 mol of adenosine 5'-triphosphate. This high yield suggests that although serine was the major source of carbon, other amino acids may also be metabolized.
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PMID:Amino acid requirements of Legionella pneumophila. 676 47

Flagella were isolated from virulent Legionella pneumophila serogroups 1, 2, and 3. Antiserum made against purified serogroups 1 flagellin agglutinated live, flagellated serogroups 1, 2, and 3 but not heat-killed or nonflagellated bacteria. A single line of identity was seen in immunodiffusion slides between the flagella isolated from the three serogroups and antibody to flagellin isolated from serogroups 1, 2, and 3. Indirect immunoperoxidase staining showed that antibody to flagellin isolated from serogroup 1 organisms reacted with flagella on serogroup 1, 2, and 3 bacteria. Indirect immunoperoxidase staining was also showed that antibody to flagellin isolated from serogroup 1 L. pneumophila did not react with the serogroup-specific cell surface antigen, thus demonstrating that the flagella- and the serogroup-specific antigen are separate antigens. The amino acid content of the flagella from the three serogroups was essentially the same, with aspartate, glutamate, alanine, and threonine comprising 41% of the total. Thirty-five percent of the amino acids were hydrophobic, and there were not detectable amounts of cysteine, tryptophan, or tyrosine.
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PMID:Immunological and biochemical relationships among flagella isolated from Legionella pneumophila serogroups 1, 2, and 3. 679 82

Because of culture medium constitution and growth conditions of Legionella species, antibiotic Minimal inhibitory Concentration (MIC) determination is difficult and does not fit with the accepted standards. The following strains have been tested : references strains : Staphylococcus aureus Oxford CNCM 53154, S. aureus 209P CNCM 53156, E. coli CNCM A224, E. coli CNCM 7324. L. pneumophila, 9 strains representative of the 6 serogroups. L. micdadei, L. dumoffii. 5 clinical isolates of L. pneumophila serogroup 1 (3 isolated in our Laboratory and 2 in Belgium). MIC of 17 antibiotics have been determined, among them macrolids not tested before. The method used was the Agar Dilution Method, with several media compared. MIC values are different in function of the various media tested Charcoal yeast extract medium, which is recommended for Legionella growth, does not inhibit activity of penicillin G, cefalotin, cefoxitin, amikacin, erythromycin and lincosamins, in contrast of tetracyclin, gentamicin, dibekacin, chloramphenicol, oleandomycin, pristinamycin, spiramycin and rifampicin. Charcoal, ferric pyrophosphate and L-cystein present in the culture medium are inactivating factors. However our results confirm good activity of rifampicin, erythromycin, cefoxitin, chloramphenicol, gentamicin and amikacin. Among the others macrolids tested, pristinamycin is the most active.
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PMID:[Practical problems raised by antibiotic minimal inhibitory concentration determination in Legionella species (author's transl)]. 681 Feb 85

We describe a new species of Legionella represented by 10 strains isolated from industrial cooling towers. Legionella oakridgensis differed genetically from the other seven species of Legionella in DNA hybridization studies and differed serologically in direct fluorescent-antibody tests. The new species, unlike all other species except L. jordanis, did not require added L-cysteine for growth in serial transfer on charcoal-yeast extract agar. L. oakridgensis, as well as three other species tested, required L-cysteine for primary isolation from animal tissues. L. oakridgensis was the only species of Legionella that failed to produce alkaline phosphatase at pH 8.5. In all other respects, it resembled other species of Legionella, including having a high content of branched-chain cellular fatty acids and being pathogenic for guinea pigs. These bacteria have not yet been associated with human disease, but they are potential causes of legionellosis.
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PMID:Legionella oakridgensis: unusual new species isolated from cooling tower water. 683 Feb 17

Ten liquid media were compared under standard conditions for their ability to support the growth of Legionella pneumophila. Modified gonococcal-ferric cysteine broth (without sodium chloride) supplemented with 1% yeast extract yielded the best overall growth of the one strain of L. pneumophila examined. Growth rates were independent of pH changes which occurred during incubation. The growth rates of 10 different strains of L.pneumophila were compared in this medium. There appeared to be little difference in the growth rates of strains passaged frequently or infrequently, or between environmental and clinical isolates. Moderate aeration resulted in a faster growth rate and in approximately a 1 log10 higher final cell concentration as compared to a static broth culture. These experiments demonstrate that there are moderate to marked differences among the various media described in the literature and that no liquid medium yet developed supports rapid growth of L. pneumophila incubated without shaking.
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PMID:Comparison of liquid growth media for Legionella pneumophila. 703 31

We found that sodium selenate added to F-G cysteine iron agar enhanced the growth of Legionella pneumophila (Philadelphia 1 strain), with visible colonies at 18 h of incubation. The optimum enhancement of growth was found to occur at a concentration of 5 to 10 microgram of selenium per ml as sodium selenate; enhanced growth, up to 22 times the number of colonies on F-G cysteine iron agar without selenate, was observed at 36 h.
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PMID:Selenium-enriched medium for Legionella pneumophila. 741 99


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