Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0023241 (
Legionella
)
6,990
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Legionella
pneumophila
is an environmental bacterium, an opportunistic premise plumbing pathogen that causes the
Legionnaires' disease
.
L. pneumophila
presents a serious health hazard in building water systems, due to its high resistance to standard water disinfection methods. Our aim was to study the use of photodynamic inactivation (PDI) against
Legionella
. We investigated and compared the photobactericidal potential of five cationic dyes. We tested toluidine blue (
TBO
) and methylene blue (MB), and three 3-
N
-methylpyridylporphyrins, one tetra-cationic and two tri-cationic, one with a short (CH
3
) and the other with a long (C
17
H
35
) alkyl chain, against
L. pneumophila
in tap water and after irradiation with violet light. All tested dyes demonstrated a certain dark toxicity against
L. pneumophila
; porphyrins with lower minimal effective concentration (MEC) values than
TBO
and MB. Nanomolar MEC values, significantly lower than with
TBO
and MB, were obtained with all three porphyrins in PDI experiments, with amphiphilic porphyrin demonstrating the highest PDI activity. All tested dyes showed increasing PDI with longer irradiation (0-108 J/cm
2
), especially the two hydrophilic porphyrins. All three porphyrins caused significant changes in cell membrane permeability after irradiation and
L. pneumophila
, co-cultivated with
Acanthamoeba castellanii
after treatment with all three porphyrins and irradiation, did not recover in amoeba. We believe our results indicate the considerable potential of cationic porphyrins as effective anti-
Legionella
agents.
...
PMID:Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen
Legionella pneumophila
. 3273 16
