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Query: UMLS:C0023241 (Legionella)
 6,990 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The infectious strain L. pneumophila serogroup 1 Philadelphia (ATCC 33152) was cultured on charcoal dialysed yeast extract agar medium (CDYE agar) which produces more virulent strains than those grown on classical agar media. The aerosol was dispersed in a depression chamber by means of a nebuliser and the density was controlled by a density probe. Male albinos Dunkin-Hartley guinea pigs weighing 250-300 g were exposed for 30 minutes to an aerosol dose of 1 LD50 (10(3) viable organisms) and 10 LD50 (10(4) viable organisms). Erythromycin lactobionate (Abbott) was administered subcutaneously 18 hours after the infection, at dosages of 270 mg/kg/day for 4 days in the animals treated with 1 LD50 and for 6 or 7 days in the animals treated with 10 LD50. The guinea pigs were observed for 9 days (weight, rectal temperature; serological and bacteriological tests (cardiac blood, lungs, spleen) and erythromycin assays (serum, lungs) were performed and compared in the treated animals, the non-treated infected control animals and the control animals which only received erythromycin. The percentage survival in the treated guinea pigs after inhalation of 1 LD50 and 10 LD50 (2 tests) were 100%, 75% and 87.5% respectively. Three weeks after treatment, the survivors had antibody titres from 32 to 1,024; the bacteriological cultures and erythromycin assays were negative. In this study, an improvement in the treatment of experimental Legionnaires' disease was observed in comparison with previous experiments. The increased dosage and duration and the early initiation of treatment resulted in survival rates of 75%.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Treatment with erythromycin of experimental legionellosis in guinea pigs infected by aerosol]. 353 58

Three diagnostic selective media used for the isolation of Legionella pneumophila were compared. These included BCYEAalpha (Oxoid, a reference medium), BCYEAalpha (Hi Media), and elective legionellosis medium (ELM) developed at the Rostov-on-Don Research Institute for Plague Control. The virulent L. pneumophila strain Philadelphia-1 (LD50 was 10(5) CFU for guinea-pigs) was used a test culture. The susceptibility of the media was determined, by culturing 10(-6) and 10(-7) dilutions of the suspension of the macerated Legionella-infected guinea-pig spleen, as well as the suspension of a culture of this strain (100 CFU) and 6 L. pneumophila cultures freshly isolated from water. The BCYEAalpha (Oxoid) and ELM media demonstrated the similar growth characteristics (chi2 < 0.7; p = 0.05) while the BCYEAalpha (Hi Media) medium showed a low susceptibility. The BCYEAalpha (Oxoid) and ELM media were first found to be successful in detecting Legionella in viable, but nonculturable state, induced by the following factors: 1) starvation in distilled water; 2) exposure to hydroquinone (oxidative shock, and 3) elevated temperature (56 degrees C). The BCYEAalpha (Oxoid) and EML media did not differ either in their ability to suppress extraneous microflora and to maintain stable initial pH under the conditions of incubation of culture plates, as well as in their Na+ concentration (15-19 mmol/l). However, the BCYEAalpha (Oxoid) medium exceeded the ELM one in the growth rate and diameter of Legionella colonies. Two L. pneumophila cultures were isolated in the BCYEAalpha (Oxoid) and EML media used in the field experiment studying 15 water samples from different hot water supply systems. Thus, the conclusion can be drawn that the EML medium is comparable with the reference BCYEAalpha (Oxoid) medium in its susceptibility and ability to detect Legionella in both vegetative and viable, but nonculturable states and is suitable for practical application.
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PMID:[Comparative study of the biological properties of various nutrient media used for the isolation of Legionella pneumophila]. 2000 Jan 16