Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0023241 (Legionella)
 6,990 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

When a single case of legionnaires' disease is reported, it should be investigated to check whether or not it is linked to other cases or part of an outbreak. The investigation includes confirmation of the diagnosis, tracing the patient's movements during the incubation period, and reporting the case to the National Surveillance Scheme for Legionnaires' Disease at the PHLS Communicable Disease Surveillance Centre. If no common factors are identified between the cases and other cases reported previously, no further action is usually required, unless it is suspected that the infection was acquired in hospital. In these circumstances, the individual case and the hospital's water maintenance programme should be reviewed, and a search made for associated cases, because hospital patients are particularly susceptible to infection. Further steps may be necessary if the link with the hospital is confirmed.
Commun Dis Rep CDR Rev 1994 Sep 16
PMID:Investigating a single case of Legionnaires' disease: guidance for consultants in communicable disease control. 752 75

Antibody engineering provides the potential to clone and manipulate antibody genes to produce fragments with altered specificity. We have produced an anti- Legionella single chain fragment with broader specificity towards Legionella serotypes than the parent monoclonal antibody. Using this relationship between the parent monoclonal and the recombinant antibody derived from it as a model, we attempted to identify those residues responsible for this change in fine specificity. Sequence analysis of this recombinant antibody revealed the deletion of a conserved residue, Asp101, in the CDR-H3 region. Using site-directed mutagenesis, we have created a mutant form of this single chain fragment with an aspartic acid insertion mutation at position 101 of the antibody heavy chain. This mutant scFv demonstrates improved specificity compared to the wild-type recombinant antibody, indicating an important role for Asp101.
 ...
PMID:Altering the fine specificity of an anti-Legionella single chain antibody by a single amino acid insertion. 1129 89