Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0022716 (
Menkes
)
1,057
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The interaction was studied of ceruloplasmin (Cp, EC 1.16.3.1), a copper-containing plasma protein, with two synthetic peptides P15 and
P16
whose structures correlate with those of the noncytosolic regions of the copper transfer P1 type ATPase (ATP7A), apparently encoded by the
Menkes disease
gene (Atp7a). Pentadecapeptide P15 and hexadecapeptide
P16
were synthesized using the solid phase method. They correspond to fragments of two extracellular loops ATP7A, of which one loop is apparently involved in the copper ion transfer (
P16
) whereas the other is not (P15). The protein footprinting showed that
P16
binds to a fragment of the ceruloplasmin domain 6. Kinetics of the ceruloplasmin-
P16
binding was studied by affinity chromatography on
P16
immobilized on a macroporous disk, and the Kd value (1.5 x 10(-6) M) of this interaction was determined. The ATP7A involvement in the copper ion transfer to nonhepatocyte cells is discussed.
...
PMID:[Identification of a fragment of ceruloplasmin, interacting with copper-transporting Menkes ATPase]. 1104 Sep 94
