Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0022568 (keratitis)
 5,133 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The ability of complement-dependent antibody to mediate destruction of rabbit corneal cells infected with the RE and KOS strains of HSV-1 was examined. Three sources of target cells were used: BHK-21, a monitor of surface antigen expression and cell lysis; SIRC, a continuous cell line of rabbit corneal origin; stromal keratocytes, grown from the middle layer of excised rabbit corneas. Rabbits were infected intrastromally with the RE strain, which causes a high incidence of stromal keratitis, and sacrificed at designated times. Results showed that all three cell types, infected with either HSV strain, expressed surface antigens and were susceptible to lysis by antibody produced during the course of stromal disease. 51Cr-release assays showed that cytolytic antibody appeared as early as 5 days postinfection and reached maximum by day 14-20. Findings suggest a direct relationship between the in vitro demonstration of complement dependent cytolysis of infected stromal cells and the disease process in experimental animals.
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PMID:Antibody mediated destruction of keratocytes infected with herpes simplex virus. 627 87

Acanthamoeba spp. are free-living protists widely distributed in environment, able to cause keratitis, encephalitis and skin lesions in humans and animals. Acanthamoeba spp. exist in two forms: an infective trophozoite and a dormant cyst. Several factors contribute to the pathogenesis of Acanthamoeba spp. The parasite adhesion to the host cell is the primary step for infection and is mediated by a mannose binding-protein, expressed in the surface and considered the main pathogenicity factor in Acanthamoeba spp. So far, there was no evidence of another surface protein of Acanthamoeba spp. relevant for host invasion or infection by these organisms. The aims of this study were to identify and characterize an Acanthamoeba castellanii surface protein and to evaluate its diagnostic potential. In silico predictions of surface proteins allowed to identify the A. castellanii calreticulin as a possible surface antigen. The coding sequence of a predicted extracellular domain of A. castellanii calreticulin was cloned by in vivo homologous recombination and the recombinant polypeptide (AcCRT29-130) was produced. Its immunodiagnostic potential was assessed in a recombinant antigen-based ELISA with sera from experimentally infected rats that developed keratitis and encephalitis, and sera from patients with encephalitis. The AcCRT29-130 was significantly more recognized by sera from encephalitis infected rats in comparison with the non-infected controls. Human sera from encephalitis patients, however presented no significant response. These results showed the AcCRT29-130 potential for A. castellanii infection immunodiagnosis in animals, with further studies being required for assessment of its use for human infections.
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PMID:Evaluation of the immunodiagnostic potential of a recombinant surface protein domain from Acanthamoeba castellanii. 2743 Feb 95