Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0022568 (keratitis)
 5,133 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To determine the corneal pathogenicity of certain anaerobic bacteria, Bacteroides fragilis keratitis was induced in rabbits by the intrastromal inoculation of 10' viable organisms. All eyes inoculated developed central abscesses within 24 hours. Abscesses persisted and became vascularized in two of three eyes that were observed for two weeks, as demonstrated both clinically and histologically. Eyes inoculated superficially with live organisms or intrastromally with solutions of dead organisms did not develop inflammatory lesions. Anaerobically incubated blood agar plates and thioglycollate broth were equally efficient in recovering organisms, although longer incubation times were occasionally necessary to recover organisms from broth cultures. Bacteroides fragilis and other anaerobic bacteria should be considered in the differential diagnosis of bacterial keratitis, and specific methods should be used to recover these organisms.
 ...
PMID:Experimental Bacteroides fragilis keratitis. 71 20

During a nine-year period ending in 1977, we scraped and cultured 663 corneal ulcers. Of these cases of keratitis, 238 were bacterial infections, 133 were fungal, and 292 were culture-negative. Pseudomonas was the predominant bacterial organism, and Fusarium was the most common fungus isolated. November was the peak month for both bacterial and fungal keratitis. Direct inoculation of multiple media, including Sabouraud's agar, blood agar, chocolate agar, thioglycollate liquid, and brain-heart infusion liquid, enhanced the recovery rate; each medium provides special nutrients for different organisms. The Gram and Giemsa stains were satisfactory, but the newer Grocott methenamine silver stain for fungi and the limulus lysate test for gram-negative bacteria proved to be clinically useful. The clinical profile of patients with fungal keratitis differed from that of patients with Pseudomonas keratitis. Thirty-three percent of the patients with Pseudomonas keratitis were wearing contact lenses at the time of their infection, and the remainder had a high incidence of predisposing ocular conditions. Fungal keratitis tended to occur in healthy male patients who had been subjected to outdoor trauma.
 ...
PMID:Spectrum of microbial keratitis in South Florida. 739 57

In this study, the response of Dectin-1 on macrophages to Fusarium solani (F. solani) and the expression patterns of Dectin-1 in experimentally F. solani-induced keratomycosis were investigated. Peritoneal macrophages isolated after intraperitoneal injection of sodium thioglycollate were co-cultured with laminarin and spores of F. solani for 12 h. The expression levels of Dectin-1 and CARD9 were detected by immunofluorescence and real-time quantitative polymerase chain reaction. A mouse model of fungal keratitis was established by substromal inoculation with spores of F. solani. Corneal lesions and inflammatory responses were observed by slit-lamp and histopathology at 1, 2, 3, 5, and 7 day(s) after infection. Dectin-1 expression was significantly upregulated on macrophages stimulated by spores of F. solani. Dectin-1 was not detected in normal corneas of C57BL/6 mice, but detected in infected corneas from the first day after inoculation, with high mRNA levels observed on days 2 and 3. CARD9, a key transducer of Dectin-1 signaling, was also upregulated in infected corneas. In conclusion, Dectin-1 is an important recognition receptor in F. solani-induced keratitis, but the molecular mechanisms warrant further investigation.
 ...
PMID:Fusarium solani Activates Dectin-1 in Experimentally Induced Keratomycosis. 3007 65