Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0022116 (ischemia)
 91,303 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Critical lower limb ischemia is a common cause for amputation. To develop new therapeutic strategies, more information is needed about molecular mechanisms of tissue responses to ischemic stress and factors inducing angiogenesis. Using a DNA array of 8400 genes, gene expression patterns in human skeletal muscle samples collected from lower limbs amputated due to acute-on-chronic or chronic critical lower limb ischemia, were compared with the control samples collected from the same limb. The results were confirmed by RT-PCR and immunohistochemistry. In acute-on-chronic ischemia, 291 genes were significantly upregulated and 174 genes were downregulated (change in 5.5% of all genes) as compared to control samples. Significant induction of the hypoxia-inducible angiogenic pathway involving hypoxia-inducible factor-1alpha (HIF-1alpha), HIF-2alpha, vascular endothelial growth factor (VEGF) and its angiogenic receptor VEGFR-2, as well as tumor necrosis factor-alpha (TNF-alpha) with its downstream signaling machinery promoting inflammation and cell death, were found in acute-on-chronic ischemia. In chronic critical ischemia, gene expression changes were much less striking than in acute-on-chronic ischemia, with 74 genes significantly upregulated and 34 genes downregulated (change in 1.3% of all genes). In the chronic situation, the anabolic and survival factors, insulin-like growth factor-1 (IGF-1) and IGF-2, were upregulated in atrophic and regenerating myocytes together with attenuated HIF, VEGF, and VEGFR-2 expression in the same cells. In conclusion, acute-on-chronic and chronic human skeletal muscle ischemia result in distinct gene expression patterns. These findings may be of importance in the design of novel therapies, such as therapeutic vascular growth, for patients suffering from lower limb ischemia.
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PMID:HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array. 1513 59

Critical lower limb ischemia is a term used to define those patients with chronic ischemic pain at rest, ulcerations or limb necrosis caused by confirmed atherosclerotic lesions of the arteries. In this paper to evaluate of the clinical usefulness of silver coated prostheses in critical lower limb ischemia was presented. The use of the vascular artificial prostheses (dacron, dacron with velour, dacron albumin coated, polytetrafluoroethylene, dacron gentamicin/ryfampicin coated) in the critical ischemia limbs threatened is her contagion. In cases of potentially threatened a contagion is indicated the use of vascular prostheses about enlarged resistance on the contagion, e.g. the silver/collagen coated prosthesis. A foundation of the use of such prostheses are antibacterial proprieties of polyester fibers salts of the silver. An aim of many works is estimation of healing of the silver prostheses of grafted in lower limb at ill with the critical ischemia where infecting of the synthetic material is extremely probable. The healing of the silver prosthesis rated is most often at the use of the scintigraphy with Technetium 99. One attends that such research will make possible the estimation of the usefulness of the vascular silver prostheses in reconstructive operations in the vascular surgery, particularly in cases threatened with the infection of used synthetic material.
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PMID:[The use of silver/collagen coated vascular prostheses in the treatment of critical limbs ischemia]. 1795 51