Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0022116 (ischemia)
 91,303 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The present study describes for the first time the use of the fluorescent dye sulfonefluorescein for intravital microscopy and microphotometry on the renal surface of rats. With help of this dye tubular secretion can be observed in mammalian kidney in vivo. Tubular secretion can further be quantified with microphotometrical measurements. The molecular structure of sulfonefluorescein is closely related to phenol red, which is known for its tubular secretion. Clearance experiments also show a secretion of sulfonefluorescein. The secretion can be inhibited by probenecid. Ureter ligation causes a strong increase in tubular concentration of the dye. After a temporary ischemia dye accumulation in tubular lumen is greatly reduced.
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PMID:Renal test dyes IV. Intravital Fluorescence microscopy and microphotometry of the tubularly secreted dye sulfonefluorescein. 98 19

We report the results obtained in a experimental work designed to evaluate the consequences of warm ischemia in hypothermic isolated renal perfusion. We perfused a number of kidneys after a period of 45 min of vascular occlusion. An alternative group of kidneys were perfused without previous warm ischemia. Ureter was canulated in all the procedures and output collected during the HP. Creatinine was added to the perfusion solution initially in order to determine creatinine clearance. HP hydrodynamics was recorded on real time through a computerised system. According to the results, renal vascular resistance as well as CrCl were higher in ischemic kidneys. Both facts along with minimal differences in pathologic study suggest an increase in vascular tone of efferent-postglomerular arteriole during HP. HP was an adequate technique to minimize histologic consequences of ischemia. Mycrovascular an biochemical changes produced during HP may be produced, essentially, by dynamic causes.
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PMID:[Normothermic ischemia effects in renal microcirculation]. 1630 4