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Query: UMLS:C0021843 (bowel obstruction)
 9,927 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This experiment was carried out as a preliminary study, an attempt to grow new intestinal mucosa on human amniotic membrane in the terminal ileum in 37 rabbits. After ketamin sulfate anesthesia at laparatomy, 5-cm ileal defects were patched with human amniotic membrane (5 x 2 cm). These patched intestines were investigated on the first postoperative day and the 2nd, 5th, 10th, and 20th weeks corresponding to 4, 5, 5, 10, and 10 rabbits, respectively. Only three rabbits died in the early postoperative period. There was no evidence of intestinal obstruction or dilatation with barium meal. Microscopically, the neomucosa consisted of a thin layer of columnar epithelial cells at 2 weeks with more maturity of the villi and less irregularity and branching by 20 weeks. All patches were covered with neomucosa commencing at 2 weeks and covering the whole patch area by 20 weeks. This technique's advantages are the large size and the ease of the availability of the human amniotic membrane for neonates at risk without jeopardizing the neonates tissues. It is hoped that this method might be considered when neonatal material is scarce.
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PMID:Human amniotic membrane as an intestinal patch for neomucosal growth in the rabbit model. 162 30

Functional intestinal mucosa, termed neomucosa, will grow in patched intestinal defects, but intestinal patching does not result in significantly increased intestinal surface area. Our aim was to determine if neomucosal growth would occur longitudinally in serosa-lined tunnels and to evaluate the optimal type of tunnel construction. Thirty rabbits had serosa-lined intestinal tunnels 1.5 cm in diameter and 5 cm in length interposed in the ileum. Group I (n = 5) had the tunnel formed from adjacent colon segments. Group II (n = 15) had a similar tunnel created with placement of an intraluminal latex stent. Group III (n = 5) had the tunnel formed by imbricating the cecum. Group IV (n = 5) had the tunnel formed by imbrication plus the intraluminal stent. Only Group II animals survived beyond 7 days (12 of 15 vs 0 of 5, P less than 0.05) and were sacrificed at 1 week (n = 3), 2 weeks (n = 3), 3 weeks (n = 2), 4 weeks (n = 2), and 6 weeks (n = 2). All other animals died from intestinal obstruction or peritonitis. Neomucosal growth occurred from proximal and distal margins. Forty percent of the tunnel was epithelialized at 6 weeks and the 5 cm tunnel contracted to 3 cm. In vitro glucose uptake was similar in neomucosa and normal mucosa but disaccharidase activity (sucrase and maltase) was significantly less in neomucosa at 6 weeks (28 +/- 35 vs 84 +/- 12 and 72 +/- 51 vs 471 +/- 84, P less than 0.05). Although not clinically obstructed, the survivors lost 15% body weight and proximal intestinal diameter increased.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Neomucosal growth in serosa lined intestinal tunnels. 235 86

Small bowel neomucosa has been grown on a variety of surfaces. The purpose of this study was to compare the rate of growth and function of neomucosa on colon serosa (CS) and abdominal wall muscle (AM) in New Zealand white rabbits. The terminal ileum was incised for 5 cm and patched with either adjacent CS (23 animals) or AM (19 animals) to create a 2 X 5-cm defect. Gross and histologic examinations of the specimens at 1, 2, 4, and 8 weeks revealed that the rate of growth was similar in both groups. There was minimal lateral ingrowth at 2 weeks, nearly complete coverage of the defects at 4 weeks, and complete coverage of the defect at 8 weeks in more than 85% of animals with mature villi and muscularis mucosae. The ileal diameter at the site of patching increased in both groups from 11.9 +/- 2.6 to 16.3 +/- 3.2 mm in the CS group and 11.3 +/- 2.5 to 15.1 +/- 1.8 mm in the AM group (P less than 0.01). Glucose uptake was similar in both groups being 65.4 +/- 24.1% of control in the CS group and 73.9 +/- 29.8% in the AM group. Brush border enzyme activity of sucrase, maltase, and lactase was similar to controls in the AM group but in the CS group activity of sucrase and maltase were significantly less than controls (P less than 0.01). Average body weight was increased postoperatively in both groups. There was one anastomotic leak in each group and two cases of partial intestinal obstruction in the abdominal wall group.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Comparison of techniques for growing small bowel neomucosa. 623 14