Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0021843 (
bowel obstruction
)
9,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Malignant
bowel obstruction
(MBO) is a serious complication which causes high death rate and low quality of life (QOL) for patients diagnosed at an advanced stage of ovarian cancer. RNA interference (RNAi) could be a promising method for the treatment of ovarian cancer and could decrease the morbidity of MBO.
Gab2
gene is overexpressed in ovarian cancer compared with normal ovarian tissue, and regulates the migratory behaviors and E-cadherin expression via activation of the PI3K pathway in ovarian cancer cells. Here, chitosan-polyethyleneimine (PEI, M
w
1800) copolymer nanoparticles were synthesized as nanocarriers to deliver
Gab2
siRNA into SKOV3 cells. The silencing effects against the
Gab2
gene and the antitumor effects by the chitosan-PEI-
Gab2
siRNA nanoparticles (chitosan-PEI-
Gab2
NPs) were studied. Results showed that highly efficient silencing effects against
Gab2
expression and its downstream effector, AKT protein, at more than 90% deregulation were obtained by chitosan-PEI NP mediated
Gab2
siRNA delivery, so as to exhibit obvious antitumor effects against SKOV3 cells with low cytotoxicity, and induce cell apoptosis in early and late stages. The study will provide novel strategies to overcome MBO in ovarian cancer by the efficient knockdown of
Gab2
expression.
...
PMID:Highly efficient Gab2 siRNA delivery to ovarian cancer cells mediated by chitosan-polyethyleneimine nanoparticles. 3226 69
