Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
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Target Concepts:
Gene/Protein
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Query: UMLS:C0021831 (
enteropathy
)
4,403
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative
enteropathy
in domestic and experimental animals. In this study, we improved the in vitro cultivation method of L. intracellularis to increase the passage efficiency and showed that L. intracellularis isolated from a rabbit and a pig have different antigenic properties. Bacteria should be recovered from infected cells before cell death due to infection to obtain higher bacterial passage efficiency, and measurement of
LDH
activity in the cell culture medium was useful for determining the timing of bacterial passage. L. intracellularis isolated from the rabbit and pig showed different band patterns in immunoblotting. Our results should be helpful in the development of serological diagnosis and epidemiological investigation methods.
...
PMID:Cultivation and characterization of Lawsonia intracellularis isolated from rabbit and pig. 1868 49
Coeliac disease (CD) is a chronic, immune-mediated small intestinal
enteropathy
, accompanied with gluten-triggered oxidative damage of duodenal mucosa. Previously, our research group reported an increased mucosal level of the antioxidant protein Parkinson's disease 7 (PARK7) in children with CD. In the present study, we investigated the role of increased PARK7 level on the epithelial cell and mucosal integrity of the small intestine. The presence of PARK7 was investigated using immunofluorescent staining on duodenal mucosa of children with CD and on FHs74Int duodenal epithelial cells. To investigate the role of oxidative stress, FHs74Int cells were treated with H
2
O
2
in the absence or presence of Comp23, a PARK7-binding compound. Intracellular accumulation of reactive oxygen species (ROS) was determined by DCFDA-based assay. Cell viability was measured by MTT,
LDH
, and Annexin V apoptosis assays. Disruption of cytoskeleton and cell adhesion was investigated by immunofluorescence staining and by real-time RT PCR. Effect of PARK7 on mucosal permeability was investigated
ex vivo
using intestinal sacs derived from control and Comp-23-pretreated mice. Comp23 treatment reduced the H
2
O
2
-induced intracellular accumulation of ROS, thus preserving the integrity of the cytoskeleton and also the viability of the FHs74Int cells. Accordingly, Comp23 treatment increased the expression of antioxidants (
NRF2
,
TRX1
,
GCLC
,
HMOX1
,
NQO1
), cell-cycle regulators (
TP53
,
CDKN1A
,
PCNA
,
BCL2
,
BAX
), and cell adhesion molecules (
ZO1
,
CDH1
,
VCL
,
ITGB5
) of H
2
O
2
-treated cells. Pretreatment with Comp23 considerably decreased the small intestinal permeability. In this study, we demonstrate that PARK7-binding Comp23 reduces the oxidative damage of duodenal epithelial cells, via increased expression of NRF2- and P53-regulated genes. Our results suggest that PARK7 plays a significant role in the maintenance of mucosal integrity in CD.
...
PMID:PARK7 Diminishes Oxidative Stress-Induced Mucosal Damage in Celiac Disease. 3296 95
