Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0021345 (infectious mononucleosis)
 3,358 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The aim of this study was to elaborate a method of heterophile mononucleosis antigen preparation useful for latex coating. This antigen was isolated from bovine red blood cells stroma by the technique of Schwarzweiss and Tomcsik with author's own modification, in which introductory extraction of erythrocytes stroma ++ was performed by means of trichloracetic acid, aqueous extraction and elution of active substance with 80% ethanol. Besides of heterophile antigen preparation obtained by the method of Schwerzweiss and Tomcsik (preparation S-T) two serologically++ active preparations were obtained (fraction I and IV), which ability to inhibit PBD agglutinating reaction and bovine red blood cells haemolysis was 16 and 8 times lower, respectively, than S-T preparation. The preparation of heterophile mononucleosis antigen obtained differed in latex coating efficacy. In order to prepare latex reagent MZ-I (from fraction I) a solution of preparation of 125 micrograms/ml concentration was used, for MZ-II (from fraction IV)--50 micrograms and for MZ-III (from preparation S-T)--15 micrograms/ml. The reagent MZ-I showed, the highest activity in agglutinating test with human serum containing heterophile mononucleosis antibodies while two others reacted with 2-4 times lover serum dilutions. Similar differentiated reactivity with these reagents was found in latex test with 15 sera from patients suspected of having infectious mononucleosis.
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PMID:[Heterophile infectious mononucleosis antigen used in the latex diagnostic test. I. A method of antigen isolation and its serologic activity]. 196 93

Fifty infectious mononucleosis (IM) and 150 non-infectious mononucleosis (non-IM) sera were tested by a hemadsorption immunocapture test (HIT) for the detection of heterophile antibodies of IgM, IgA, and IgE classes. The specificity of Paul-Bunnell (PB) antibodies was ascertained by a differential absorption test. IgM PB-antibodies were demonstrated in 100% of IM sera, IgA in 92%, and IgE in 88%. PB antibodies were present only in IM sera; but other heterophile antibodies of IgM (68%), IgA (6.7%), and IgE (9.3%) classes were demonstrated in non-IM sera. IgA and IgE heterophile antibodies were thus present, especially in IM sera. HIT was found to be more sensitive than Paul-Bunnell Davidsohn test (PBD test) and suitable for the diagnosis of infectious mononucleosis.
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PMID:Heterophile IgM, IgA, and IgE antibodies in infectious mononucleosis. 254 44

Ten rapid slide agglutination tests for infectious mononucleosis were evaluated. The quality of the technical information included with the kits was found to differ considerably, as did the composition of the tests and the principles of test specificity. Furthermore, large variation was found in the shelf life and price of the different kits. Thirty-nine serum samples were selected according to titre in the Paul-Bunnell-Davidsohn differential absorption test, largely from the low titre area, since this is where most diagnostic problems can be expected. The sera were tested blindly using the ten rapid slide agglutination tests. Considerable differences were found in specificity and sensitivity. When compared with the results obtained from PBD, the tests could be divided into two groups. The first group consisted of three kits, all of which gave false negative, but no false positive results. The remaining seven tests gave false negative and false positive results, but were more sensitive than the tests in the first group. The large variation in the results obtained when using the different kits under controlled conditions indicates that such tests may give even less satisfactory results in practice.
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PMID:Evaluation of ten commercial heterophile antibody tests for infectious mononucleosis. 384 Mar 18

The study was aimed at establishment whether dependence exists between severity of clinical course of infectious mononucleosis and the level of heterophilic antibodies in the PBD test. Antibody titers were measured three times during illness in 34 persons with infectious mononucleosis, divided into two groups: I--patients with moderate course of the disease and II--patients with moderate-severe course. In five persons out of 34 tested negative PBD test result was obtained, although these patients exhibited symptoms on mononucleosis confirmed by other laboratory methods. Positive result of the PBD test was occurring more frequently and mean titer of heterophilic antibodies was higher in patients with moderate clinical course than in persons with moderate-serious course of the disease. Decrease of PBD test titer was observed between first and third determination in both groups of patients.
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PMID:[Dynamics of the Paul Bunnel-Davidsohn test during infectious mononucleosis]. 818 13

A case of 26-years old man with symptoms of infectious mononucleosis syndrome is presented. In the course of the disease: high temperature, weakness, loss of appetite, sore throat, myalgia, hepatomegaly, splenomegaly and some laboratory changes (leucocytosis with presence of atypical lymphocytes, elevated aminotransferase activity) have been observed. Serological tests have shoved: slighthly positive PBD-test in the first examination (second-negative) and the presence of IgM antibodies against CMV in a high titer with four-time decrease of the titer during the course of the disease. Because of the incomplete symptom complex for infectious mononucleosis caused by EBV we have put the diagnosis of cytomegaly coursed as a infectious mononucleosis syndrome.
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PMID:[A case of infectious mononucleosis syndrome caused by cytomegalovirus]. 823 51