Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0021051 (immunodeficiency)
 71,517 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Bone marrow transplantation (BMT) is often followed by significant morbidity and mortality due to protracted immunodeficiency. We have hypothesized that the bone marrow-ablative regimen may delay the recovery of normal immune function following transplantation by impairing the interaction of host endothelial cells with circulating graft-derived lymphocytes. This report compares the relative effects of busulfan (an alkylating drug) and gamma-irradiation on the tissue-specific localization potential of lymphocytes and the eventual recovery of immune function within syngeneic murine transplant recipients. Localization of normal lymphocytes into peripheral lymph nodes of irradiated BMT recipients was markedly less (less than 50%) than in busulfan-treated or normal mice over the first 2 months post-BMT. This finding correlated with irradiation-induced endothelial cell edema and microvascular occlusions within lymphocyte-receptive areas of the nodal microvasculature. The effect of both preparative regimens on the recovery of contact hypersensitivity (CHS) was also analyzed. This response recovered more quickly (between 1 and 2 months) in busulfan-pretreated animals. Further experiments demonstrated that the decrease in CHS responsiveness appeared, in part, related to a depression in the capacity of lymphocytes to localize into skin sites of antigen deposition within irradiated mice. The impairment of tissue-specific lymphocyte localization may represent a novel mechanism by which whole body irradiation can contribute to delayed immunologic reconstitution following bone marrow transplantation.
 ...
PMID:Recovery of contact hypersensitivity responses following murine bone marrow transplantation: comparison of gamma-irradiation and busulfan as preparative marrow-ablative agents. 331 44

The clinical manifestations of chronic infection with the Human Immunodeficiency Virus (HIV) fall into two broad categories: opportunistic infections and opportunistic malignancies. The initial observation of both occurring in outbreak fashion among young homosexual men led to the early identification of the present pandemic. Conversely, the identification of additional malignancies which occur in excess frequency in the presence of the immunodeficiency of HIV infection can provide insight into the role of viruses in human malignancy. The first report related to the acquired immunodeficiency syndrome (AIDS) epidemic was of a series of 5 cases of Pneumocystic carinii in young homosexual men in Los Angeles and was published in June 1981. This was shortly followed by the report of additional cases of P. carinii as well as Kaposi's sarcoma (KS) occurring among young homosexual men in California and New York City. The increased risk of KS among people with HIV infection has been confirmed since these initial reports, with 1 in 5 AIDS patients in the United States developing KS sometime in their course of disease. However, the proportion of AIDS patients with KS has decreased from 35% before 1983 to 15% in the first half of 1987. Among the recognized risk groups of AIDS patients, the proportion with KS is highest among homosexual men and female intravenous drug abusers, and lowest among children and hemophiliacs. This variation suggests that risk of KS in AIDS parallels that of sexually-transmitted infections. A second family of opportunistic malignancy in AIDS is comprised of the non-Hodgkin's disease lymphomas (NHL). These lymphomas are typically of B-cell origin, immunoblastic or Burkitt's-like in character, and frequently present with extra-nodal involvement such as the central nervous system. These were first recognized somewhat later than KS as being associated with AIDS; together, KS and NHL account for about 95% of all neoplasms seen in AIDS patients. Additional malignancies are currently suspected to occur excessively with HIV infection. These include Hodgkin's disease, anorectal carcinoma, and testicular cancer. Validation of these associations will require extensive epidemiologic surveillance. Since HIV infection leads to progressive loss of cellular immunity, it is probable that these malignancies result from the progressive reactivation or loss of immunologic control of latent oncogenic viruses. The cytomegalovirus has been implicated in the pathogenesis of KS, perhaps with reinfection, and the Epstein-Barr virus in the NHL. The role of papilloma viruses in anorectal carcinoma has also been proposed.(ABSTRACT TRUNCATED AT 400 WORDS)
 ...
PMID:Opportunistic malignancies and the acquired immunodeficiency syndrome. 350 39

We report two examples of an unusual expression of fatal granulomatosis with primary lymph node location, characterized histologically by numerous giant cells of the Langhans' type, effacement of the nodal structure, mixed lymphohistiocytic infiltrate, and marked histiocyte cell maturity, all features which initially did not exclude the possibility of a granulomatous process of infectious origin. The clinical features of these cases include cellular or combined immunodeficiency, a possible family history and a rapidly fatal course. The present article emphasizes the great similarity which can exist between histiocytic and granulomatous disorders, the great variability of histiocytic diseases from the pathological view-point, the presence of borderline examples, and the several overlapping features of different entities. Specifically, the most remarkable finding of these two cases is the existence of numerous mature giant cells of the Langerhans' type within a massive obliteration of the nodal structure. Furthermore, these giant cells characterized by a strikingly orderly disposition of peripheral nuclei in a wreath-like pattern, frequently suggesting the structure of Touton's giant cells.
 ...
PMID:Giant cell granulomatosis of lymph nodes with a fatal course. 641 37

A new "nude" mouse model was developed by successive crossings and backcrossings between athymic nu/nu mice, on an N:NIH(S) background, and female CBA/N mice that have an X-linked immune defect in B-lymphocyte function. The resulting doubly congenic N:NIH(S(II-nu/nu mice maintained the marked thymic hypoplasia and poor development of hair of the nu/nu mice. In contrast to nu/nu and CBA/N mice, in this new mouse model both T-cell zones of lymph nodes and the spleen were depleted of lymphocytes. Lymphocytic follicles were rare and diminutive; not germinal centers were noted. The nodal cortical and paracortical areas were represented principally by connective tissue, endothelial cells, and macrophages, including giant multinucleated cells. No medullary cords were recognized. The mice with combined immunodeficiency supported the growth of human tumor xenografts and were susceptible to murine viral hepatitis.
 ...
PMID:N:NIH(S)-nu/nu mice with combined immunodeficiency: a new model for human tumor heterotransplantation. 696 28

Epstein-Barr virus (EBV) is a gamma DNA herpes virus which is thought to play a part in the pathogenesis of some non-Hodgkin's lymphomas in individuals with or without immunodeficiency. We investigated 16 lymph nodal and 12 cutaneous anaplastic large cell lymphomas (ALCLs) (Ki-1+), all of which were in patients without immunodeficiency, for the presence of EBV genomes. The highly sensitive polymerase chain reaction (PCR) technique was employed for detection of viral DNA in extracts from formalin-fixed, paraffin-embedded tissue sections. In addition, we performed radioactive and non-radioactive in situ hybridization (ISH) for localization of EBV at the single cell level. EBV-DNA was demonstrated by PCR in five cases of nodal ALCLs (31%). All cutaneous ALCLs were negative. EBV-encoded small nuclear RNAs (EBERs) could be identified by ISH in the tumour cells of one of the five EBV-DNA-positive patients. Our results further support the concept that EBV may be involved in the development of a proportion of nodal ALCLs, but not in cutaneous ALCLs.
 ...
PMID:Detection of Epstein-Barr virus in cutaneous and lymph nodal anaplastic large cell lymphomas (Ki-1+). 757 80

The t(2;5)(p23;q35) translocation is associated with a high percentage of anaplastic large-cell lymphomas (ALCL) of T- or null-cell phenotype. This translocation was recently cloned and results in the fusion of the nucleophosmin gene (NPM) on chromosome 5q35 to a novel tyrosine kinase-encoding gene designated anaplastic lymphoma kinase (ALK) on chromosome 2p23. Using a sensitive and specific reverse transcription-polymerase chain reaction (RT-PCR) assay to detect the NPM/ALK fusion transcript, we assessed the involvement of NPM/ALK in a series of histologically and immunohistochemically confirmed ALCL, in non-ALCL aggressive non-Hodgkin's lymphomas of T-cell phenotype, and in Hodgkin's disease (HD) to better define the morphologic spectrum of disease associated with this translocation. Twenty-four cases of ALCL were selected on the basis of CD30 positivity and histologic features. Seventeen cases presented as classical nodal and extranodal disease, four cases presented as primary cutaneous disease, and three were associated with human immunodeficiency virus (HIV) infection. As ALCL may show overlapping histology with both HD and other aggressive non-Hodgkin's lymphomas, particularly of T-cell phenotype (T-NHL), we also studied 34 cases of HD and 19 of T-NHL. NPM/ALK chimeric transcripts of identical size were detected in 11 of the 24 (46%) cases of ALCL. NPM/ALK fusion transcripts were found in 11 of 17 (65%) classical ALCL cases but were not detected in the four primary cutaneous cases of ALCL or in the three HIV-related ALCL cases. In addition, NPM/ALK transcripts were not detected in any of the 34 cases of HD or in the 19 cases of T-NHL. These data indicate that NPM/ALK fusion transcripts occur in a high percentage of classical nodal ALCL (65%). In addition, these data strongly suggest that ALCL, as defined in this study, is not pathogenetically related to either HD disease or the majority of other types of aggressive T-NHL. This is a US government work. There are no restrictions on its use.
 ...
PMID:Analysis of the t(2;5)(p23;q35) translocation by reverse transcription-polymerase chain reaction in CD30+ anaplastic large-cell lymphomas, in other non-Hodgkin's lymphomas of T-cell phenotype, and in Hodgkin's disease. 766 79

Rather than central tolerance, the perinatal inoculation of related F1 hybrid spleen cells into inbred mice may result in host-versus-graft (HVG) reactions manifested as transient autoimmunity, or as a lethal immunodeficiency syndrome. RFM/(T6xRFM)F1 chimaeras with lethal disease die in 30 days with lymphosplenomegaly, immune complexes and impaired immune responses. The present studies used in vitro proliferation assays to show that the HVG reaction caused hyperplasia sufficient to account for the lymphosplenomegaly, while also causing severe impairment of splenic and nodal cell responses to concanavalin A (Con A) and to bacterial lipopolysaccharide (LPS). By 25 days, HVG mice could not distinguish between self and non-self as judged by mixed lymphocyte reactions (MLR) to RFM, (T6xRFM)F1 and third party A/J cells. There were no indications that host cells reactive to F1 donor cells had undergone clonal deletion, anergy or expansion. Flow cytometry revealed that donor T lymphocytes achieved stable engraftment, mostly in the nodes, despite the HVG reaction. Taken together with previous observations, these studies showed that HVG reactions in young parent F1/chimaeras can result in an immunodeficiency state which is characterized by an early appearing, profound and persistent impairment of both host and donor T and B cell functions. The results suggest that HVG reactions can contribute directly to immune deficits seen after clinical allogeneic bone marrow transplantation.
 ...
PMID:Immunodeficiency in RFM/(T6xRFM)F1 mouse chimaeras with lethal host-versus-graft syndrome. 774 76

Diagnosis of tuberculosis in patients infected with the human immunodeficiency virus (HIV) is sometimes difficult because of atypical clinical and radiographic findings. The aim of this retrospective study was to determine the utility of a gallium-67 citrate scan (67Ga scan) of the chest for the early diagnosis of tuberculosis in patients infected with HIV. We selected 174 67Ga scans performed as a part of the clinical evaluation of 145 HIV-infected patients with normal pulmonary parenchyma (seen on chest radiographs) and fever of unknown origin. Scans were evaluated as to whether there was uptake in lymphoid regions (a positive 67Ga scan) or not (a negative scan). Tuberculosis was the most common condition associated with a positive 67Ga scan (48[72.7%] of 66 positive 67Ga scans). Nodal uptake had a 72.7% positive predictive value and a 92.6% negative predictive value for tuberculosis. In our experience, 67Ga scanning is a useful tool for the clinical evaluation of HIV-infected patients with unexplained fever. In areas with a high prevalence of tuberculous infection, a 67Ga scan of an HIV-infected patients that shows nodal uptake allows the clinician to initiate prompt empirical antituberculous therapy while waiting for culture results. Conversely, a 67Ga scan that does not show nodal uptake makes the diagnosis of tuberculosis unlikely.
 ...
PMID:Utility of the gallium-67 citrate scan for the early diagnosis of tuberculosis in patients infected with the human immunodeficiency virus. 775 91

Lymph nodes from cats with natural feline immunodeficiency virus (FIV) infection were examined histopathologically for characterization of lesions in the symptomatic and terminal stages. The localization of viral antigen was also studied in the nodes by immunohistochemistry using a monoclonal antibody to FIV p24. In a node of persistent generalized lymphadenopathy stage, follicular hyperplasia was prominent and the viral antigen was demonstrated predominantly in the follicular lymphocytes and tingible body macrophages. In cats died at AIDS related complex stage, the lymph nodes showed either mixed follicular hyperplasia and involution, involution, or mixed involution and depletion. The lymph nodes in those died at AIDS stage showed considerable destruction of the nodal architecture with involution and depletion of lymphoid follicles. In this terminal stage, the viral antigen was seen prominently in histiocytes/macrophages of the sinus. These findings observed in the dead cats were similar to the lymph node changes seen in human AIDS associated with HIV infection. It was suggested that sequential histologic changes from follicular hyperplasia to depletion via involution took place in the FIV infected lymph node with persistence of FIV antigen.
 ...
PMID:Histopathology and viral antigen distribution in lymph nodes of cats naturally infected with feline immunodeficiency virus. 794 83

We investigated 81 cases of peripheral pleomorphic T-cell lymphoma (PMTCL) occurring in human immunodeficiency virus-negative Europeans for the presence of Epstein-Barr virus (EBV)-DNA through polymerase chain reaction (PCR) for the presence of EBV-encoded small nuclear RNAs (EBER) and immediate early mRNAs (Bam H-fragment, lower strand frame [BHLF]) by in situ hybridization (ISH) and for EBV-encoded latent membrane protein (LMP) and nuclear antigen 2 (EBNA2) by immunohistology (IH). EBER-ISH, which could be applied on all cases, showed an overall incidence of EBV-infected cells in 38 of 81 cases (47%) of PMTCL. These data could be confirmed by PCR, which produced congruent results in the cases with amplifiable DNA. By EBER-ISH, the virus was located in the tumor cells in 30 of the 38 EBV-positive cases, with the proportion of the infected cells ranging from 1% to 100%. In 18 of these cases and in the 8 cases without EBV-infected tumor cells, the virus was, respectively, either additionally or exclusively detectable in occasional nonmalignant lymphoid bystander cells. An LMP expression was observed in several of the EBER-expressing tumor cells in 18 cases, whereas EBNA2 was detectable only in one case, which also displayed signs of viral replication. Some nonmalignant EBV-infected B immunoblasts also expressed LMP in several cases. Primary cutaneous and enteropathy-associated PMTCL displayed less frequent EBV infection when compared with other extranodal or nodal manifestations.
 ...
PMID:Frequent latent Epstein-Barr virus infection of neoplastic T cells and bystander B cells in human immunodeficiency virus-negative European peripheral pleomorphic T-cell lymphomas. 839 74


<< Previous 1 2 3 4 5 6 7 8 9 Next >>