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Query: UMLS:C0020672 (hypothermia)
 17,327 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The aim of the study was to estimate the effect of hypothermia on (125J)-iodoinsulin binding to liver plasma membranes. Rat liver membranes were prepared from control, normothermic rats (Tr = 35.6 +/- 0.3 degrees C) and hypothermic rats (Tr = 26.5 +/- 0.9 degrees C) and purified according to Havrankowa. In addition, serum insulin and glucagon levels by means of RIA and glucose concentration using the glucose oxidase method were measured. Scatchard analysis was used to determine the kinetic parameters of the hormone receptor interaction. The data showed no significant differences in the affinity of the binding sites but indicated a decrease in receptor concentrations in liver plasma membranes from hypothermic rats. In contrast to changes in serum insulin level which was decreased by about 50% in hypothermic rats blood glucose concentrations did not significantly differ between the hypothermic and normothermic ones. Our results show that in hypothermic rats the hormonal adaptation operates on the level of the number of liver receptors whereas the insulin receptor affinity remains unaffected.
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PMID:Effect of hypothermia on the insulin--receptor interaction in liver plasma membranes. 812 87

The aim of the study was to investigate the effect of hypothermia on (125J)-insulin binding to rat skeletal muscle membranes and to determine whether the decrease in blood insulin concentration could be related to changes in the number or in the affinity of insulin receptor sites according to the down-regulation theory. Rat skeletal muscle membranes were prepared from control, normothermic rats (Tr = 35.6 +/- 0.3 degree C) and hypothermic rats (Tr = 26.0 +/- 0.5 degree C) and purified according to Havrankowa. In order to determine the kinetic parameters of the hormone-receptor interaction the data from the competition binding studies were analysed by the method of Scatchard using the LIGAND Pc.v.3.1. computer program of Munson and Rodbard. We have shown that under hypothermic conditions insulin receptors number is significantly increased in specific hindlimb skeletal muscles but the changes take place mainly in the low affinity receptors class. The phenomenon probably results from the lack of spare high affinity insulin receptors in skeletal muscle as shown recently by Camps et al.
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PMID:Effect of hypothermia on the insulin-receptor interaction in skeletal muscle plasma membranes. 880 58

Experimental hypothermia caused extensive changes in the number of both classes of insulin receptors in different rat tissues. In the liver, the number of high affinity insulin receptors (HAIRs) decreased by 50% (from 25.3 to 12.6 fmol/mg membrane protein), whereas number of low affinity insulin receptors (LAIRs) was almost unchanged in comparison to normothermic animals (5.63 and 4.39 pmol/mg, respectively). In the adipose tissue, number of both classes was reduced--HAIRs by 81% (from 24.0 to 4.50 fmol/mg) and LAIRs by 92% (from 16.0 to 1.29 pmol/mg). In the skeletal muscle, capacity of HAIRs was not changed (16.2 and 19.3 fmol/mg in normo- and hypothermic animals, respectively), whereas number of LAIRs increased by 150% (from 6.65 to 16.6 pmol/mg). Hypothermic rats also showed lower amount (by 85%) of LAIRs in the heart muscle (9.37 and 1.43 pmol/mg in control and experimental animals, respectively). Simultaneously, no significant changes were found in HAIRs (16.3 and 11.9 fmol/mg, respectively) and LAIRs (4.43 and 3.88 pmol/mg, respectively) in the brain. These differences in insulin receptors responses to hypothermia may reflect different physiological role of insulin in the regulation of target cell metabolism and/or the differences in tissue distribution of the insulin receptor isoforms.
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PMID:Effect of hypothermia on insulin-receptor interaction in different rat tissues. 1223 18

A 60-year-old woman presented to her primary care physician with fatigue and anemia. Laboratory evaluation revealed a hemoglobin level of 9.8 g/dL and an erythrocyte sedimentation rate (ESR) of 64 mm/hour. She subsequently developed nocturnal episodes of diaphoresis, confusion, and hypothermia. Capillary glucose measurements during the spells revealed hypoglycemia. During two supervised fasts, the patient's plasma glucose levels fell to 35 mg/dL and 32 mg/dL, respectively. Plasma insulin and C-peptide levels were appropriately suppressed, but a low concentration of beta-hydroxy-butyrate and normal increase of plasma glucose concentration after a glucagon injection suggested the presence of an insulin-like substance. Computed tomographic (CT) scan of the abdomen and subsequent positron emission tomographic (PET) scan revealed extensive lymphadenopathy. Biopsy of periaortic lymph nodes revealed Hodgkin's disease of the mixed cellularity type. Following chemotherapy, a complete remission ensued, the spells abated, and hypoglycemia was not induced by a 23-hour fast. We believe that the patient's Hodgkin's disease was producing an insulin-like substance. The observations of others suggest that this substance may be an autoantibody to the insulin receptor.
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PMID:Recurrent hypoglycemia and hypothermia in a patient with Hodgkin's disease. 1719 56