Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0020538 (hypertension)
 170,190 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Hybridomas were established from cell fusions between mouse myeloma cells and mouse or rat spleen cells immune to human and rat bladder tumors. Hybridoma supernatants were screened for their immunospecificities against human bladder tumor tissues and urine samples from bladder cancer patients. Eight monoclonal antibodies including 2 rat antibodies were selected and their diagnostic values were assessed. Four mouse monoclonal antibodies termed IMb1, IMb4, IMb9 and PMe were shown to react with 40 to 80% of human bladder tumor tissues examined in this investigation. No relationship was found between the antigen expression and clinical stage of the disease. Four other antibodies termed S31, S85, HBP-1 and RBM-1 reacted with antigens in urine from patients with bladder tumor and other types of uropathy, in contrast to their low reactivities against urine from healthy donors.
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PMID:[Analysis of bladder cancer-associated antigens and their application to diagnosis]. 360 46

White Carneau pigeons, prone to atherosclerosis, were selectively bred for either high (HBP) or low (LBP) blood pressure to study blood pressure-atherosclerosis interaction. Sixty-four HBP and 45 LBP pigeons, of both sexes and 16-30 months of age, were used to evaluate spontaneous atherosclerosis and the response to a moderately atherogenic diet. At 0 time, HBP pigeons had a greater percentage of aortic intima covered with fatty streaks and a lesser involvement with raised plaque. After 4 and 6 months of dietary treatment, the HBP pigeons with plasma cholesterol concentrations elevated only 22 and 8% above basal level responded with greater increases in the extent and severity of raised plaque whereas LBP pigeons changed little. On the average, medial thickness and medial areas of the atherosclerosis-free proximal aorta were greater in HBP compared to LBP at all times. Lower compliance of the aorta in the HBP line at 6 months (0.49 +/- 0.027 microliter/mm Hg/cm thoracic aorta) compared to LBP (0.57 +/- 0.036) suggested stiffer arterial walls in the HBP line. The findings suggest that HBP pigeons selected for increased blood pressure are more responsive to moderate hypercholesterolemia acting as a stimulus for atherosclerosis progression. The HBP pigeon line is therefore a useful animal model in which to investigate genetically derived hypertension-atherosclerosis interactions.
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PMID:Spontaneous and diet-aggravated atherosclerosis in White Carneau pigeons genetically selected for discordant blood pressure. 360 32

A principle objective in chemotherapy is the development of modalities capable of selectively destroying malignant cells while sparing normal tissues. One new approach to selective photochemotherapy, antibody-targeted photolysis (ATPL) uses photosensitizers (PS) coupled to monoclonal antibodies (MAbs) which bind to cell surface antigens on malignant cells. Selective destruction of human T leukemia cells (HBP-ALL) was accomplished by coupling the efficient PS chlorin e(6) to an anti-T cell MAb using dextran carriers. Conjugates with chlorin: MAb ratios of 30:1 retained > 85% MA b binding activity, and had a quantum yield for singlet oxygen production of 0.7 +/- 0.1, the same as that of free chlorin e(6). Cell killing was dependent on the doses of both MAb-PS and 630-670 nm light and occurred only in target cell populations which bound the MAb. On the order of 10(10) singlet oxygen molecules were necessary to kill a cell. A second approach to specific photochemotherapy, selective carcinoma cell photolysis (SCCP), relies on preferential accumulation of certain cationic PS by carcinoma cell mitochondria. We have evaluated several classes of cationic dyes, and in the case of N,N'-bis-(2-ethyl-1,3-dioxolane)-kryptocyanine (EDKC) and some of its analogs, have demonstrated highly selective killing of human squamous cell, bladder and colon carcinoma cells in vitro. In isolated mitochondria, EDKC uptake and fluorescence depended on membrane potential, and the dye specifically photosensitized damage to Complex I in the electron transport chain. N,N'-bis-(2-ethyl-1,3-dioxolane)-kryptocyanine and some of its analogs accumulated within subcutaneous xenografts of human tumors in nude mice with tumor:skin ratios > 8. Photoirradiation caused significant inhibition of tumor growth, without cutaneous phototoxicity.
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PMID:Strategies for selective cancer photochemotherapy: antibody-targeted and selective carcinoma cell photolysis. 361 36

Na+ interaction with unsealed human red cell ghosts has been studied by 23Na-NMR relaxation rate (R1) measurements. Data on a total of nine subjects including seven volunteer normotensives (NBP) and two untreated hypertensives (HBP) are presented. Qualitative treatment of the data gives information on the dynamic behavior of Na+ undergoing fast exchange between the free and bound states. The excess longitudinal relaxation rate (delta R)-1 plotted against total [Na+], known as the James-Noggle plot, exhibits different behavior for NBP and HBP ghosts, with a relatively low binding constant of approx. 100 M-1 for HBP (p less than 0.025) compared to a high constant of 500-1000 M-1 for NBP. To associate our NMR data with membrane-bound (Na+ + K+)-ATPase, 23Na relaxation rates were measured in the presence of 5 mM ouabain. James-Noggle plots constructed for ouabain-sensitive excess relaxation rates show the binding for NBP to be even high affinity (greater than 10(3) M-1) but low capacity. These data may suggest that for a given amount of intracellular Na+, the binding affinity could determine the distribution of Na+ between the membrane and cytoplasm, and that the (Na+ + K+)-ATPase which is primarily responsible for the Na+ affinity might assume an abnormal transport mechanism in HBP membranes.
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PMID:23Na-NMR studies of Na+ interaction with human red cell membranes from normotensives and hypertensives. 370

To investigate the affinity of glucocorticoid (GC) to its receptor, the binding of [3H]hydrocortisone 17-butyrate 21-propionate ([3H]HBP) and [3H]dexamethasone ([3H]DEX) in rat liver was analyzed kinetically. Scatchard analysis of [3H]hydrocortisone ([3H]HC) binding yielded a curvilinear plot with upward concavity with high and low affinities. The dissociation constant (Kd) value of high affinity was 1.9 nM and of low affinity 68.7 nM. A Scatchard plot of [3H]HBP binding showed a straight line with high affinity. The Kd value was 9.8 nM. The Kd values for the low affinity site of HC were in good agreement with the Ki values obtained from displacement experiments of [3H]DEX and [3H]HBP binding. The Ki values of HC for [3H]DEX and [3H]HBP were 51.9 and 42.3 nM respectively. The association rate constant for HBP to the GC receptor was 2.9 times lower than that for HC. The dissociation rate constant for HBP was 6.1 to 8.3 times lower than that for HC. The Kd values for [3H]HBP (9.5 nM) and [3H]HC (30.0 nM) obtained from the above two rate constants were approximately the same as the Ki and Kd values (in the case of HC, the value of the low affinity site). These results suggest that esterification of the hydroxyl group(s) in the side chain of GC by butyrate and propionate increased the affinity to the GC receptor, and that a decrease in the dissociation rate from the receptor caused the increase in the affinity to the GC receptor.
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PMID:Enhancement of affinity to receptors in the esterified glucocorticoid, hydrocortisone 17-butyrate 21-propionate (HBP), in the rat liver. 371 38

Hodgkin's disease-derived giant cell lines (HD-cells) express high levels of ectosialyltransferase activity presumed to be a galactose-specific lectin recognizing the desialylated 3-fucosyl-N-acetyllactosamine structure (X-hapten). Both the anti-X-hapten monoclonal antibody VIM-D5 and a polyclonal antiserum to another galactose-lectin, the hepatic asialoglycoprotein receptor (HBP), recognize a 55,000-mol wt HD-cell protein (Paietta, E., R. J. Stockert, A. G. Morell, V. Diehl, and P. H. Weirnik. 1986. Proc. Natl. Acad. Sci. USA. 83:3451-3455.) That the expression of the 55,000-mol wt protein is restricted to HD-cells among X-hapten positive cells lines is confirmed in this study. The 55,000-mol wt protein is shown to be present on the cell surface and intracellularly, where an additional immunocrossreactive 150,000-mol wt protein is recognized. Extraction of the 55,000 mol wt protein from HD-cell lysates by affinity chromatography results in the loss of sialyltransferase activity. While evidence for a single protein possessing both the antigenic and the enzymatic activity is not direct, these results suggest that the ectosialyltransferase unique to HD-cells is a 55,000-mol wt membrane glycoprotein possessing the X-hapten oligosaccharide.
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PMID:Unique antigen of cultured Hodgkin's cells. A putative sialyltransferase. 373 96

The present study is a proton NMR investigation of the influence of Ca2+ on the interaction between the intact sialic acid residues on HBP (Hepatic Binding Protein) and methyl-beta-D-galactoses. The proton NMR spectra of HBP and methyl-beta-D-galactose mixing solution at different Ca2+ concentration have been measured. The analysis of the experimental results indicates that Ca2+ participates in the binding of sialic acid residues on HBP with methyl-beta-D-galactoses and this enables the galactose molecules to be in stable bound state. The proton NMR spectra of the samples containing EDTA have confirmed this conclusion.
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PMID:1H NMR investigation of the binding of methyl-beta-D-galactoses with sialic acid residues on hepatic binding protein--the effect of divalent calcium ions. 374 68

A mitogenic heparin-binding (reactive) lectin-like protein (HBP) was purified from the extract of a cloned rat thymic myoid cell R615B2 by a one-step procedure of affinity chromatography on a heparin--Sepharose CL-6B column. Four distinct peptide bands with molecular weights of 10,000, 13,000, 13,700, and 14,600 were detected on SDS-polyacrylamide gel electrophoresis. This protein is mitogenic at concentrations of as low as 1.1-70.0 ng/ml for peanut lectin-nonagglutinated thymocytes and splenocytes from euthymic mice and rats but not for splenocytes from nude mice. These results indicate that thymic myoid cell-derived HBP is an important signal for one particular step in T-cell differentiation.
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PMID:Mitogenic heparin-binding lectin-like protein from cloned thymic myoid cells. 380 7

Forty-five patients with diastolic blood pressure (DBP) greater than or equal to 105 mmHg were randomly assigned to receive Western (group 1, n = 21) or a classical Chinese herbal preparation (group 2, n = 24) to treat their hypertension (HBP). All remained hypertensive after 4 days in the hospital without treatment. Except for baseline Na+ excretion (higher in group 1) and somewhat more evidence of end organ damage in group 1, the patient groups were comparable. Those in group 1 were given a thiazide diuretic and propranolol if needed, and those in group 2, a mixture of 12 herbs. Patients on active therapy in group 1 had a drop in blood pressure (BP) from 172.6 +/- 27.8/107.4 +/- 13.6 to 141.2 +/- 26.2/89.6 +/- 12.0 mmHg, whereas those in group 2 had no change in BP, 168.8 +/- 22.0/107.7 +/- 9.8 mmHg to 165.7 +/- 23.7/106.0 +/- 11.8 mmHg. Although 66% of patients in group 1 had a DBP under 90 mmHg by discharge, only 8% of those in group 2 did. Except for a fall in serum K+ in group 1, there were no significant biochemical or clinical problems in either group. We conclude that standard Western medication is more effective than a classical Chinese herbal preparation used to treat HBP.
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PMID:A comparison of the treatment of hypertension with Chinese herbal and Western medication. 380 53

The aim of this work was to assess if biochemical alterations are present in catecholaminergic neurons of genetically hypertensive mice (HBP strain) compared to genetically hypotensive mice (LBP strain), as well as to mice of the population (R) from which HBP and LBP strains have been selected. For that purpose, dopamine (DA), noradrenaline (NA) and adrenaline (A) concentrations were determined by high performance liquid chromatography with electrochemical detection, in peripheral structures and brain nuclei of adult male mice from these three strains. In the adrenal medulla of HBP mice, DA, NA and A concentrations are increased when compared to R, but not to LBP mice. In the superior cervical ganglia of HBP mice, the NA concentration is decreased when compared to LBP, but increased when compared to R mice. At the central level, the NA concentration is decreased in HBP mice when compared to LBP, but not significantly changed when compared to R mice, in the following regions: locus coeruleus (-39 p. 100, p less than 0.001), A1-C1 (-17 p. 100, p less than 0.001) and A2-C2 (-19 p. 100, p less than 0.001) areas of the medulla oblongata, thoracic spinal cord (-26 p. 100, p less than 0.01), posteroventral hypothalamus (-18 p. 100, p less than 0.01) and nucleus hypothalamicus anterior (-14 p. 100, p less than 0.01). In the nucleus periventricularis, NA concentration of HBP mice is not changed when compared to both LBP and R animals. Finally, in the caudate nucleus of HBP mice, the DA concentration is decreased when compared to both R and LBP mice.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Central and peripheral catecholamines in the genetically hypertensive mouse]. 393 39


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