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Query: UMLS:C0020473 (hyperlipidemia)
 15,891 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Hyperlipemia in an acyanotic patient with diabetic ketoacidosis, alcoholism, and pancreatitis produced a falsely elevated concentration of methemoglobin (19 percent) and a lower-than-expected oxygen saturation measured with an automated spectrophotometer (IL-282 CO-Oximeter). In addition, there was a "normal" hemoglobin level despite a low hematocrit reading. In vitro studies showed that hyperlipemia corresponding to triglyceride levels of 500 mg/100 ml and greater produced erroneously high values for methemoglobin and total hemoglobin and "negative" values for carboxyhemoglobin. These abnormalities disappeared when the excessive lipids were removed by washing the erythrocytes in physiologic saline solution.
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PMID:Factitious methemoglobinemia caused by hyperlipemia. 673

It has been demonstrated by numerous workers that variations in levels of glycolysed hemoglobins Hb Al (Alb, Alb, Alc) are an excellent means of control of the quality of diabetes control. The estimation of the main glycolysed hemoglobin (Hb Alc) is very difficult, and various chromatographic methods have been developed to permit rapid estimation of all these glycolysed hemoglobins. Our work consisted of testing the three microcolumn kits on the french market: Isolab, Helena and Biorad. The great thermo-dependency of the first two kits did not permit us to obtain results in correlation with those of our reference technic derived from Trivelli's method. On the other hand, the levels of Hb Al obtained with the Biorad kit were perfectly correlated with the levels of Hb Alc. The average normal of Hb Al was 6,45 +/- 0,66 p. cent, CV 10,2 p. cent; the average value of Hblc was 5,4 +/- 0,4 p. cent. CV 7,4 p. cent. The average levels of Hb A obtained in diabetic subjects was 12 p. cent, the levels of Hblc being 10 p. cent. Certain restrictions in the use of these microcolumns were demonstrated: presence of Hb F or abnormal Hb, hyperlipemia and presence of chylomicrons.
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PMID:[Evaluation of three commercial kits for the estimation of total glycolysed hemoglobin: Hb Al (author's transl)]. 677 65

Optimal control of diabetes should achieve not only euglycemia and normal levels of glycosylated hemoglobin but also absence of the reversible concomitants of diabetes such as red cell rigidity, hyperlipidemia, increased capillary permeability, enlargement of the kidneys, proteinuria, etc. Unfortunately, in most patients consistent euglycemia cannot be assured even with two daily injections of insulin. However, self-measurement of blood glucose as a guide to insulin taken before each meal and at bedtime can, in selected patients, increase the frequency of normal glucose levels without undue hypoglycemia.
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PMID:Parameters of good control in diabetes mellitus. 699 74

We have evaluated a fully automated thyroxine assay involving the use of a homogeneous enzyme assay and a Technicon AutoAnalyzer II continuous flow system. Comparison by regression analysis with a thyroxine radioimmunoassay kit method gave a slope of 0.82 and a y-intercept of 7.81 micrograms/L (SE 0.86 microgram/L, r = 0.95). Within-run precision yielded CVs of 1.0-6.1%, between-day CVs were 2.0-14.4%; within-day precision showed a mean variance of 7.81 micrograms/L. Mean analytical recovery was 96.1%. Bilirubin, hemoglobin, and lipemia interfered with quantitation of results when their concentrations exceeded 50 mg/L, 300 mg/L, and 5 g/L, respectively. The reference interval for euthyroid status was calculated to be 50-110 micrograms/L. Sensitivity was 5.0 micrograms/L with a mean carryover of 1.65%. Current reagent and labor costs for enzyme immunoassay ($0.40) were less than for the manual radioimmunoassay procedure ($0.40) were less than for the manual radioimmunoassay procedure ($1.70). The assay is economical, simple to perform, and amenable to high-throughput thyroid screening in the routine laboratory.
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PMID:Continuous-flow enzyme immunoassay for thyroxine in serum. 701 33

Hyperlipemia is a frequent finding in diabetes mellitus. As not only the liver, but intestinal mucosa as well synthesizes endogenous lipoproteins, we have investigated the small intestinal mucosal lipid content in 11 adult patients with juvenile onset diabetes and in 7 patients with maturity onset diabetes. Eleven non-diabetic patients served as controls. After a fasting period of 12-14 h blood was drawn for determination of glucose, lipids and glycosylated hemoglobin AI. Then several small bowel biopsies were performed by an hydraulic multiple biopsy tube or endoscopically and the specimens were processed immediately for further biochemical, histochemical and electronmicroscopical workup. Patients with juvenile and with maturity onset diabetes did not differ from controls with regard to serum lipids and to intestinal mucosal lipids determined biochemically. Surprisingly, patients with maturity onset diabetes exhibited a significantly (p less than 0,005) higher concentration of intestinal mucosal triglycerides than patients with juvenile onset diabetes. Fasting blood glucose and hemoglobin AI levels were slightly elevated in both groups of diabetic patients. Histochemically lipid particles were demonstrable in intestinal mucosa of diabetics and of controls with equal variability. The electronmicroscopical appearance of intestinal mucosa did not differ between diabetic patients and controls. Only in one patient with juvenile onset diabetes an accumulation of lipid particles within the cisternae of the Golgi apparatus was observed. In conclusion, neither biochemically, nor histochemically, nor electronmicroscopically an abnormal accumulation of lipids could be found in the small intestinal mucosa of patients with well controlled diabetes mellitus.
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PMID:[Biochemical and micromorphological investigation of lipids in small intestinal mucosa of patients with diabetes mellitus (author's transl)]. 726 11

A sensitive method (Clin. Chem. 26: 327--331, 1980) for serum iron, in which the color reagent Ferrozine is used, is modified and adapted to the Abbott ABA-100 discrete analyzer. The standard curve is linear to at least 10 mg/L and the method showed day-to-day precision (CV) of 2.4% for a 1.03 mg/L sample (n = 63) and 1.9% for a 2.13 mg/L sample (n = 63). Lower values were obtained than with the modified continuous-flow technique of Giovanniello et al., but the correlation was good (r = 0.98). Bilirubin and copper do not interfere; hemoglobin and gross lipemia interfere only slightly. The total iron-binding capacity, based on Ramsay's method, was evaluated with regard to the effect of adding various amounts of magnesium carbonate. Results led us to use a ratio of approximately 180 mg of magnesium carbonate to each 5 micrograms of excess iron added. Day-to-day, the method for total iron-binding capacity gave a CV of 3.1% for a 2.55 mg/L sample, 2.8% for a 3.63 mg/L sample.
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PMID:Ferrozine iron and total iron-binding capacity method adapted to the ABA-100 Bichromatic Analyzer. 727 7

A glycosylated hemoglobin (HbA1) test was used to evaluate the role of dialysate glucose in the development of carbohydrate intolerance and hyperlipidemia in chronic hemodialysis patients and chronic peritoneal dialysis patients. HbA1 levels were significantly elevated in all groups of patients. HbA1 levels were not ameliorated with 8 weeks of glucose-free hemodialysis. There was no correlation between HbA1 and serum glucose, triglycerides, or cholesterol. Thus, HbA1 elevation cannot be explained solely by glucose reabsorption from dialysate. This test is helpful in the detection of carbohydrate intolerance, but its usefulness in evaluation of hyperlipidemia of dialysis patients is uncertain.
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PMID:Glycosylated hemoglobin in patients treated by chronic dialysis. 732 94

We have developed a kinetic fixed-time approach for the quantitative determination of serum glucose by use of the hexokinase/glucose-6-phosphate dehydrogenase method. To achieve a large measuring range, we have apparently increased the Michaelis constant of glucose-6-phosphate dehydrogenase through addition of the competitive inhibitor ATP. By this means, serum samples with glucose concentrations up to 55.5 mmol/l could be analyzed without pre-dilution. The method has been adapted to the ENI GEMSAEC analyzer and to the LKB 2086 Mark II analyzer. It yielded satisfactory results with regard to precision. A comparison of the kinetic procedure with the manual end-point method showed good agreement. No interferences from hemoglobin, bilirubin, or lipemia have been observed.
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PMID:Kinetic determination of serum glucose by use of the hexokinase/glucose-6-phosphate dehydrogenase method. 735 90

We investigated the effect of temperature, variant hemoglobins, and hyperlipidemia on determination of glycosylated hemoglobin by an electrophoretic method (Clin. Chem. 26: 1598-1602, 1980). We found that: (a) temperature variations ranging from 4 to 30 degrees C were without effect on results obtained by electrophoresis; (b) concurrent determination of glycosylated hemoglobin by electrophoresis and column-chromatography in blood specimens from 150 diabetic patients yielded almost identical mean values for both procedures when operations were carried out at 22 degrees C; (c) electrophoretic determination of glycosylated hemoglobin in whole-blood hemolysate was not affected by concentration of triglycerides; and (d) unlike column-chromatographic procedures, which underestimate the percentage of glycosylated hemoglobin in patients with hemoglobin S and C, the electrophoretic method accurately determined the proportion of glycosylated hemoglobin in these hemoglobinopathies. Evidently, electrophoresis on agar gel is an excellent alternative to cation-exchange column-chromatographic methods for glycosylated hemoglobin.
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PMID:Agar gel electrophoretic determination of glycosylated hemoglobin: effect of variant hemoglobins, hyperlipidemia, and temperature. 747 99

Highly sensitive assays that quantitate human immunodeficiency virus type 1 (HIV-1) RNA may be valuable for clinical research and the treatment of HIV-1-infected patients. In this study we evaluated the reproducibility and accuracy of the first-generation branched DNA (bDNA-1.0) signal amplification assay under conditions that are relevant to routine use in a clinical context. We show that the bDNA-1.0 assay was able to discern two- to three-fold changes in plasma HIV-1 RNA levels as significant. Reverse transcription coupled to polymerase chain reaction (RT-PCR) was less reproducible and required a 3.7- to 5.8-fold change in plasma HIV-1 RNA levels to be statistically significant. The accuracy of the bDNA-1.0 assay in RNA quantitation was not affected by HIV-1 genotypic variation or by the presence of hemoglobin, bilirubin, lipemia, or any of a dozen therapeutic drugs. Using the bDNA-1.0 assay, we show that HIV-1 RNA levels in plasma specimens were stable when stored at -80 degrees C and were able to withstand at least three freeze-thaw cycles without significant loss. We also examined the performance of an ultrasensitive bDNA assay with improvements to the signal amplification technology. The ultrasensitive bDNA assay displayed a quantitation limit of approximately 500 RNA Eq/ml, yet maintained a dynamic quantitation range up to 1.6 x 10(6) RNA Eq/ml. Like the bDNA-1.0 assay, the ultrasensitive bDNA assay was not affected by HIV-1 genotype variability.
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PMID:Performance characteristics for the quantitation of plasma HIV-1 RNA using branched DNA signal amplification technology. 755 11


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