Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0020440 (hypercapnia)
 7,939 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The intracellular energetic environment of rat hippocampal slices was manipulated by bolstering ATP levels following the addition of adenosine to the incubation medium, or by manipulating intracellular pH. Addition of 8 mM adenosine to the incubation medium increased total tissue adenylate and ATP content, but did not prolong electrical function during anoxia. Further, it resulted in long-lasting alterations in normoxic evoked responses. Intracellular pH (pHi) was changed by manipulating the bicarbonate/CO2 ratio of the incubation medium, or by adding amiloride, a hydrogen/sodium antiport blocker. Estimates of intracellular pH using the creatine kinase equilibrium agree with those obtained by Neutral red scanning spectrophotometry in control conditions. However, only Neutral red indicated an acidification with amiloride treatment, while the creatine kinase equilibrium was preferentially affected by hypercapnia, suggesting the presence of at least two pH compartments in hippocampal brain slices. These manipulations cannot be carried out easily in vivo, and provide a means of determining the importance of metabolic changes on neural function during anoxia.
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PMID:Manipulating the intracellular environment of hippocampal slices: pH and high-energy phosphates. 272 18

We determined the regional intracellular pH (pHi) of rat brainstem in response to hypercapnia. Neutral red spectrophotometry was used to characterize changes in intracellular pH along the dorsal and ventral aspects of the medulla oblongata. Male Wistar rats (250-350 g) were infused with 3 ml of 2% Neutral Red over the course of 30 minutes. After 20 minutes of infusion, the rats were ventilated with either 7% CO2, 21% O2 in N2 or room air for 15 minutes. Our data indicate that hypercapnia induces prominent intracellular acidification in neurons within putative chemosensitive regions of the ventral aspect of medulla oblongata. On the contrary, minimal or no changes were observed in neurons within the nucleus tractus solitarius. These findings suggest that brainstem neurons differentially regulate intracellular pH during hypercapnic stress.
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PMID:Differential expression of intracellular acidosis in rat brainstem regions in response to hypercapnic ventilation. 1463 94