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Query: UMLS:C0019829 (Hodgkin's disease)
 30,247 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

PHA-induced peripheral blood lymphocyte (PBL) proliferation and T-cell colony formation in soft agar was studied in the presence of interleukin-2 (IL-2) containing medium in untreated Hodgkin disease (HD) patients and normal controls. The proliferative response of HD PBL was potentiated in presence of IL-2-containing medium in 11 of 20 cases studied, and there was marginal increase or inhibition of PHA response in 16 normal lymphocyte samples. Lymphocytes from patients in advanced stages of the disease, and those who were initially hyporesponsive responded better to suboptimal doses of PHA in the presence of exogenous IL-2. There was no improvement in the colony forming capacity of T lymphocytes from HD PBL even in the presence of IL-2-containing medium, whereas normal T-cell colony formation was augmented significantly both in number and in size of colonies.
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PMID:Effect of exogenous interleukins on in vitro responses of T lymphocytes from patients with Hodgkin disease. 349 12

13 patients with Hodgkin's disease (HD) previously treated, 9 of whom were long-time (more than 2 yr) off-therapy, were studied for peripheral blood lymphocyte response to interleukin 2 and for lymphocyte subpopulations by means of in vitro cultures and monoclonal antibodies. The aim of the study was to ascertain the role played by interleukin 2 in the impaired cell-mediated immunity of HD patients. The results show a response of peripheral blood mononuclear cells of HD patients to either the T cell-specific polyclonal mitogens PHA and Con A or to the T cell-dependent, although B cell-specific, PWM, most significantly decreased compared to the normal response. As far as the interleukin 2 involvement in HD is concerned, our study suggests: an impaired endogenous interleukin 2 production by T lymphocytes, a most probable deficiency of the interleukin 2 receptor (Tac) expression and 3) a decrease of the number and/or of the function of NK cells no longer responsive in vitro to interleukin 2. The phenotypic analysis of peripheral blood mononuclear cells showed a slight decrease of total T cells (T3+), of the helper/inducer subset (T4+) and of the T4+/T8+ cells ratio. Our data seem to support the rationale for a therapeutical approach with interleukin 2 in controlled clinical trials also in HD patients, according to the experiments in progress in solid tumor patients.
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PMID:Peripheral blood lymphocyte response to recombinant and non-recombinant interleukin 2 in previously treated patients with Hodgkin's disease, long-time off-therapy. 349 36

Patients with untreated active Hodgkin's disease (HD) have a defect in cell-mediated immunity. After therapy many HD patients still have long lasting abnormalities in T cell number and function. We examined whether NK activity is also permanently impaired in HD patients in remission. The mean NK activity of peripheral blood lymphocytes from 42 patients who were disease-free for 6-150 months was not different from that of healthy controls. Augmentation of NK activity after treatment of the cells by interferon in vitro was equal for patients and controls. Normal NK activity in HD in remission was independent of disease stage, age, remission duration and mode of therapy. Measuring PHA-induced lymphocyte proliferation and NK activity simultaneously demonstrates that patients with impaired cell-mediated immunity do not have concomitant reduction of NK activity. We conclude that NK activity in HD in remission is independent of decreased T cell mediated immunity. In addition, NK is resistant to long term suppression by the chemotherapy and radiation protocols that are used in HD.
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PMID:Normal natural killer cell activity in Hodgkin's disease patients in remission. 366 88

The study group included 113 patients in a continuing 1 to 10 year-long complete clinical-hematological remission from Hodgkin's disease. 43 of them had prior splenectomy. The latter revealed a higher level of lymphocytes (chiefly 0-cells) and a diminished response of mononuclear blood cells to PHA as compared to the other patients and healthy controls. Also, they had more lymphocytes which became capable of E-rosette formation following a short-term incubation at 37 degrees C (lymphocyte reactivation by means of surface modification). The negative correlation between the reactivated T-cells level and mononuclear cell response to PHA suggested a functional blocking of T-cells involving damage to membranes. The absence of reactivated T-lymphocytes in nonsplenectomized patients was regarded as circumstantial evidence for the role played by the spleen in withdrawing faulty lymphocytes from peripheral blood.
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PMID:[Role of splenectomy in impairing lymphocyte elimination in patients with lymphogranulomatosis]. 376 11

Histologically, lymphogranulomatosis X (LgrX) is a Morbus Hodgkin-like disease which until now has been considered as an abnormal immune reaction or a prelymphoma. Chromosome analyses showed that LgrX and angioimmunoblastic lymphadenopathy AILD) are characterized by chromosomal aberrations. Chromosome analyses of 18 cases of LgrX with sequential banding techniques showed abnormalities in 13 out of 18 cases. They were monoclonal in 7 cases. The most frequent abnormalities were trisomies of chromosomes Nos 3 and 5 and duplication of the X-chromosome. The abnormal karyotypes always appeared with normal mitosis in single or clonal cells. They were found in unstimulated and in PHA-stimulated cultures from lymph node and peripheral blood. Thus, it can be concluded that at least some cases of LgrX are monoclonal cell proliferations. An attempt is made to define the role of chromosomal abnormalities in the development of malignant lymphomas.
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PMID:Chromosomal abnormalities in lymphogranulomatosis X (LgrX)/angioimmunoblastic lymphadenopathy (AILD). 382 Nov 57

Seven patients, long-term survivors of Hodgkin's disease, and 24 of their relatives (parents, siblings and children), together with normal controls were studied for percentages, absolute counts and mitogen-proliferative responses by means of monoclonal antibodies, E rosette technique and in vitro cultures with PHA, ConA and PWM. The aim of the study was to ascertain whether the impaired cell-mediated immunity of Hodgkin's patients was also present in relatives in order to elucidate the still debated etiology of the defect and of the disease (congenital? environmental? infectious?). The results show that both Hodgkin's patients and their relatives have a significant decrease of total T cells (as T3+, T11+ and E rosette-forming cells) in peripheral blood and a significant impairment of polyclonal responses to all the mitogens employed. The Leu-7+ cells (i.e. a consistent amount of natural killer cells) are significantly increased only in the Hodgkin's patients but not in their relatives. The T cell subpopulations (T4 and T8), B cells and monocytes do not show any difference between the patients, their relatives and normal controls. Our results seem to support, at least in part, the presence of a common defect of T cell lineage both in patients and in their relatives, but its etiology still remains uncertain (genetic? environmental?).
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PMID:Quantitative and functional abnormalities of total T lymphocytes in relatives of patients with Hodgkin's disease. 387 18

Peripheral blood lymphocytes (PBL) of patients with Hodgkin's disease were studied for their capacity to produce interleukin 2 upon in vitro phytohemaglutinin stimulation in the presence or absence of either interleukin 1 or indomethacin (2 micrograms/ml); eight patients were studied at the discovery of their disease before receiving any therapy (onset HD; OHD). Seventeen patients were tested in long-term (greater than 3 yr) remission (remission HD; RHD); most RHD were treated with both chemotherapy and irradiation. Fourteen healthy individuals served as controls. PBL from OHD have a significant (P less than 0.01) defect in the production of PHA-induced IL-2. Indomethacin and IL-1 had no effect on IL-2 yield. PBL from RHD yield intermediate levels of IL-2, which are nevertheless significantly lower (P less than 0.02) than control values. RHD recover the capacity of normal PBL to increase their production of IL-2 in indomethacin-supplemented culture medium. Interestingly, PHA responsiveness was significantly decreased only in RHD, thus not explaining the low IL-2 yield obtained in supernatants. In addition, 4-day PHA-blasts from both HD patients and control individuals increase their thymidine incorporation in the presence of purified lectin-free IL-2 to a similar degree, suggesting that their IL-2 receptors are unimpaired. Finally, OHD sera significantly inhibit PHA-induced IL-2 yield of normal PBL, suggesting that a seric component(s) may play a role in some cases. We conclude that defective IL-2 production may play a role in the well-documented deficient cellular immunity seen in Hodgkin's disease.
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PMID:Defect in lectin-induced interleukin 2 (IL-2) production by peripheral blood lymphocytes of patients with Hodgkin's disease. 387 20

Chemotaxis is a property common to all free cells or unicellular microorganisms. It is not a simple spontaneous cellular migration but one which is directed towards the source or nucleus, producer of the chemotactic substance. One of the first phenomenon which is established as a defense mechanism of the organism is the attraction of polymorphonuclears. In 1955 Rebuck and Crowley described a method, "skin window" for the study of in vivo leukocyte chemotaxis. The aim of this work was to go deeper into the study of this test and to establish its clinical use. Two hundred and seventy patients from both sexes were studied and divided into five groups: Group I - 60 healthy subjects as control. Group II - 60 patients with pathologic leukocyte response: 10 cirrhotics, 15 Hodgkin's disease, 15 chronic renal insufficiency, 2 drepanocytosis and 3 sarcoidosis. Group III - 60 patients with no theoretical alterations in the leukocyte chemotaxis: 22 bronchial asthma, 23 nonlymphoid neoplasm, 13 iritis and 2 histiocytosis X. Group IV - 40 active tuberculosis patients. Group V - 30 patients with bacterial pneumonia non-tuberculosis. The Rebuck test was carried out on all patients. As lymphocyte markers, E rosettes, superficial immunoglobulins and the lymphoblast transformation test against PHA were performed on all the groups of patients. As to the results obtained, the positive responses for Groups I, II, III, IV and V were 87%, 28%, 83.3%, 45% and 63.3%, respectively. These results were evaluated in relation to the Mantoux reaction. The modified Rebuck test is useful for leukocyte chemotactic study. This was found to be altered in 13% of the healthy population.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Behavior of leukocyte chemotaxis in various clinico-immunological situations]. 389 89

The high risk for malignancy for carriers of several congenital chromosomal abnormalities is discussed. We report herein a patient with Klinefelter's syndrome associated with a high grade malignant non-Hodgkin lymphoma. Chromosome analysis of PHA-stimulated lymphocytes showed a mosaic of 46,XXY (90%) and normal 46,XY (10%) metaphases. To our knowledge, this is the third reported case of a malignant non-Hodgkin lymphoma in a patient with Klinefelter's syndrome in the literature.
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PMID:Klinefelter's syndrome and malignant lymphoma. 394 47

Lectins are proteins which have the ability to interact specifically with carbohydrate residues of glycoproteins and other glycoconjugates. The staining patterns of 10 fluorescein conjugated lectins (F-Con A, F-LCA, F-RCA, F-WGA, F-PHA, F-PWM, F-LTA, F-SBA, F-PNA, F-DB) and a protease inhibitor (F-LA) have been studied in histological sections of 11 normal or reactive lymph nodes and 6 nodes and one skin biopsy involved by Hodgkin's disease. On the basis of the patterns of lectin binding, and current knowledge of their saccharide specificities, we found that within germinal centres there is an orderly carbohydrate rich extracellular matrix which contains a higher concentration of GlcNAc and terminal Gal residues than the surface membranes of component cells. This suggests active secretion rather than simple membrane shedding, and it is possible that this pericellular domain plays a part in the regulation of the proliferative response, or controls migration of lymphocytes in and out of the germinal centre. Lectin binding in Reed-Sternberg cells suggests that the huge nucleoli contain glycoconjugates of diverse structure, which may be linked with their failure to undergo cytokinesis.
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PMID:Studies of lectin binding to normal and neoplastic lymphoid tissues. I. Normal nodes and Hodgkin's disease. 618 96


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