Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0019693 (HIV)
 170,526 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Western blot (WB) analysis of various strains of HIV-2 indicated that transmembrane glycoprotein (TMP) of HIV-2 exists as trimers. These trimers have molecular weights and electrophoretic mobilities in the region of the major external glycoprotein, gp120, resulting in WB misidentification during diagnosis. A simple and rapid procedure was developed using trichloroacetic acid (TCA) to efficiently dissociate oligomeric forms of the TMP to monomers prior to the preparation of WB. This procedure permitted the unambiguous identification of antibodies to gp120 and to the TMP. Use of HIV-2 WB strips without any oligomeric forms of the TMP demonstrated (1) that cross reactivity of HIV-1-positive specimens on HIV-2 WB was mainly directed to Gag and Pol proteins, with some reactivity to gp36/gp41 TMP, but none to gp120; (2) that these strips can substantially reduce the number of specimens falsely identified as dually (HIV-1 and HIV-2) reactive; and (3) that HIV-2-positive specimens reacted to viral gp120 in a strain-specific manner, demonstrating high antigenic variation in this glycoprotein. It is recommended that this general procedure of viral protein dissociation be used for HIV-2 WB preparation.
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PMID:Oligomeric nature of transmembrane glycoproteins of HIV-2: procedures for their efficient dissociation and preparation of Western blots for diagnosis. 177 59

It's evident nowadays, that the population of AIDS infected people will increase. In 88% cases the ENT specialists could be the first contact doctors for HIV-positive patients. This fact makes necessary for them to know as well as possible clinical symptoms, etiopathology and immunology of AIDS. The authors present in this paper some of these problems.
Otolaryngol Pol 1991
PMID:[AIDS in otolaryngology]. 179 25

The authors present the problem with HIV positive patients which have an ear, nose and throat disease. ENT doctors are the group of high risk of AIDS. Up to now we haven't got a test useful for early diagnosis in AIDS (specially this period between infection and seroconversion+--"serological window"). This fact requires special cautions from ENT surgeons++. The authors describe the methods of individual prevention from AIDS and, the surgical instruments sterilization.
Otolaryngol Pol 1991
PMID:[Otolaryngological surgical procedures and HIV infection]. 179 26

Anti-p24 and anti-gp41 antibody titers were determined in sera from 20 asymptomatic HIV carriers and 15 AIDS patients. Anti-p24 titer was found to be significantly lower in AIDS patients than in asymptomatic HIV carriers and anti-gp41 titer was similar in both investigated groups. A correlation was found between T4/T8 lymphocyte ratio and anti-p24 antibody titer in patients with AIDS.
Pol Arch Med Wewn 1991 Dec
PMID:[Titer of anti-P24 and anti-GP41 antibodies as a diagnostic parameter in patients with asymptomatic and symptomatic HIV infections]. 181 86

Material of this study consists of data collected during treatment of HIV infected patients admitted to AIDS Clinic in Warsaw. This unit, as the first in Poland, hospitalized seropositive patients with various infectious problems. Psychiatric complication of HIV infection (which needed psychotropic medication) was already observed in the first patient, who died with AIDS diagnosis (in February, 1987). In this study data concerning patients in AIDS Clinic during 1990 are presented. In this time 89 HIV seropositive patients (11 of these were women) were admitted to the clinic. Fifty two patients (10 of them were women) were IV drug abusers. Other 33 patients were homosexual (among them 4 IV drug abusers). Eight patients were out of high risk groups for HIV infection. Twelve of 89 patients were newly AIDS diagnosed. Three of them were IV drug abusers. Nine of AIDS patients (one a drug abuser) died in 1990. In six of 12 patients with AIDS diagnosis (50%) neuropsychiatric disorders were stated: depressive syndromes (n = 2), dementia syndromes with severe neurological symptoms (n = 2), delirium syndromes (n = 2). Other 3 person were IV drug abusers. In this study all above mentioned cases and their treatment are described. Our data suggest that HIV seropositive patients with psychiatric disorders need very careful diagnosis and an individual therapeutic intervention.
Psychiatr Pol
PMID:[Neuropsychological disorders in patients with symptomatic AIDS treated in 1990 at the Acquired Immunodeficiency Syndrome Clinic, Medical Academy, Warsaw]. 182 87

In 588 sera of blood donors the Western Blot test (WB) was done for confirming the presence of anti-HIV-1 antibodies. Negative results were obtained in 247 sera and positive in 90. In 251 cases the result was doubtful (42.7%). These results were obtained in 153 cases, however only 104 subjects came for control tests and had from 1 to 5 tests. Eight subjects were returned to the register of blood donors after obtaining of two negative WB results. In 5 cases in control test the serum reacted with the protein of HIV-1, and they were regarded as being carriers. In the remaining 91 subjects doubtful results persisted for from 3 to 12 months. The reactivity of the doubtful sera changed. Most of them reacted with the proteins opceded by one of three genes, they were mostly core proteins, among them p24 protein. The remaining sera reacted with proteins encoded by genes of the envelope and core. The possible causes of these doubtful results are discussed.
Acta Haematol Pol 1991
PMID:[Dubious results of determining the presence of anti-HIV-1 antibodies in blood donors]. 182 53

The polybren test (P) was used parallelly with the antiglobulin test (PTA-LISS) and enzymatic test (LEN) for detection of HIV-1 antibodies. The analysis of the results of testing of 646 sera showed that the P test is a simple method making possible detection of most antibodies of the IgG class without using antiglobulin serum within up to 3 minutes. The sensitivity of the P test was high. The sera with antibodies to the antigens of the systems Rh, Kidd, Duffy, MNSs and other less frequent antibodies such as anti-Vel, anti-Coa anti-Co(b), anti-Dia and anti-Ge showed the highest activity in the P test. In one serum anti-D antibodies, in three sera anti-C and in one serum anti-Ge antibodies were detected only in the P test, while no reactions were obtained in the PTA-LISS and LEN tests. Among the immune antibodies the exception was the antibody to Kell antigens which weakly reacted in the P test and in most cases an additional phase of testing with antiglobulin PTA-P was necessary for its demonstration. The P test was of low usefulness in the detection of cold IgM antibodies such as anti-Le, anti-Fe1, anti-I and anti-HI.
Acta Haematol Pol 1991
PMID:[Evaluation of the test using polybrene for the detection of anti-erythrocyte antibodies]. 182 54

We have analyzed the structure and expression of the HIV-1 vif and vpr mRNAs. The results revealed that the predominant vif and vpr mRNAs belong to the intermediate size class of HIV-1 mRNAs and that their expression is dependent on the presence of Rev protein. In addition, low levels of a small multiply spliced vpr mRNA were produced by HIV-1. cDNA cloning and expression of vpr cDNAs in eucaryotic cells revealed that high levels of Vpr were produced only from the intermediate-size mRNA in the presence of Rev. Thus, as demonstrated for the viral structural proteins, expression of Vif and Vpr is regulated by Rev. The arrangement of the splice sites and the Rev-RRE interaction are responsible for the regulation of viral expression, and especially for the switching from an early stage, producing only or primarily Tat, Rev, and Nef from multiply spliced mRNAs, to a late stage, leading to the production of Gag, Pol, Env, Vpu, Vif, and Vpr from unspliced and partially spliced mRNAs.
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PMID:Expression of human immunodeficiency virus type 1 vif and vpr mRNAs is Rev-dependent and regulated by splicing. 183 Jan 83

A novel method based on dose-response curve of antibodies and employing a hyperbolic function for its description was used for the assessment of the affinity and concentration of anti-HIV antibodies in eighteen anti-HIV positive males. The patients were followed up for a period of 8 months to 5 years and affinity and concentration of antibodies were measured at least twice, at the beginning and at the end of the observation period. No correlation between antibodies affinity and the clinical stage of infection was observed, however, concentration of antibodies decreased rapidly in AIDS.
Mater Med Pol
PMID:Affinity and concentration of anti-HIV antibodies in asymptomatic and symptomatic HIV infection. 184 30

51 BALFs from HIV positive patients were analysed. In 16 of them Pneumocystis carinii organisms were found. Two methods detecting Pneumocystis carinii were used: Grocott-Gomori, fluorescence Papanicolaou stain. The best staining method turned out to be Grocott-Gomori. The differential count was carried out in 40 BALFs. In patients with Pneumocystis carinii detected by BAL neutrophilia was always present. In some of these patients lymphocytosis was also present. In 4 patients with high lymphocytosis immunocytochemical examinations with monoclonal antibodies against CD4, CD8 T cells showed a marked decrease of the CD4/CD8 ratio.
Pneumonol Alergol Pol 1991
PMID:[Usefulness of bronchoalveolar gavage in patients with acquired immunodeficiency syndrome]. 184 41


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