Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0019204 (hepatocellular carcinoma)
 71,386 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

2-Deamino-2-methyl-N10-propargyl-5,8-dideazafolic acid (ICI 198583) is a potent inhibitor of thymidylate synthase. Its analogue, N(alpha)-[4-[N-[(3,4-dihydro-2-methyl-4-oxo-6-quinazolinyl)methyl]-N-propargylamino]phenylacetyl]-L-glutamic acid, containing p-aminophenylacetic acid residue substituting p-aminobenzoic acid residue, was synthesized. The new analogue exhibited a moderately potent thymidylate synthase inhibition, of linear mixed type vs. the cofactor, N(5,10)-methylenetetrahydrofolate. The Ki value of 0.34 microM, determined with a purified recombinant rat hepatoma enzyme, was about 30-fold higher than that reported for inhibition of thymidylate synthase from mouse leukemia L1210 cells by ICI 198583 (Hughes et al., 1990, J. Med. Chem. 33, 3060). Growth of mouse leukemia L5178Y cells was inhibited by the analogue (IC50 = 1.26 mM) 180-fold weaker than by ICI 198583 (IC50 = 6.9 microM).
Acta Biochim Pol 2002
PMID:Synthesis and biological activity of N(alpha)-[4-[N-[(3,4-dihydro-2-methyl-4-oxo-6-quinazolinyl)methyl]-N-propargylamino]phenylacetyl]-L-glutamic acid. 1213 41

Increased incidence of hepatocellular carcinoma related to hepatitis C virus (HCV) infection has been noted recently. Only in year 2000 seven new cases of HCC in HCV-positive patients were diagnosed. In all cases liver tumors were found in cirrhotic patients and they were at advanced stage (multiple or large in size) precluding successful therapy. More than half of HCC cases related to HCV infection were connected with blood transfusion(s) in the past. Patients transfused a few decades ago should be screened for HCV infection and those with liver cirrhosis require careful and regular monitoring including ultrasound and a-fetoprotein examinations in order to detect focal lesions at less advanced stage making medical intervention possible.
Pol Merkur Lekarski 2002 Aug
PMID:[Increased incidence of hepatocellular carcinoma]. 1242 Mar 36

The ribosomal protein S2 (RPS2) is encoded by a gene from the highly conserved mammalian repetitive gene family LLRep3. It participates in aminoacyl-transfer RNA binding to ribosome, potentially affecting the fidelity of mRNA translation. These studies were designed to measure the expression of RPS2 during increased cell proliferation. Using Western and Northern blot analyses, we found that the levels of RPS2 protein and its corresponding mRNA were higher in mouse hepatocellular carcinoma, in mouse livers after one-third partial hepatectomy, and in serum-starved cultured hepatocytes following serum treatment. Our study shows that the increased expression of RPS2 correlates with increased cell proliferation. However, whether the altered expression of this protein reflects its involvement in cellular proliferation or represents an associated phenomena is still a key question that needs to be explored.
Acta Biochim Pol 2002
PMID:Increased expression of ribosomal protein S2 in liver tumors, posthepactomized livers, and proliferating hepatocytes in vitro. 1242 31

In the course of anthracyclin administration, melatonin acts as an effective scavenger of oxygen free radicals and exerts cardio- and nephroprotective effects. The present study aimed at corroborating cytostatic effectiveness of daunorubicin, applied in parallel with melatonin, in rats with transplanted Morris hepatoma. The percentage of tumour cells, which manifested traits of necrosis was recorded and extent of apoptosis was evaluated in tumour cells and in cells of myocardium. Daunorubicin administration was followed by a significant increase in necrosis and apoptosis in tumour cells and by intensified apoptosis in myocardium cells. Melatonin administered in parallel with daunorubicin decreased the extent of necrosis in tumour cells and reduced the proportion of apoptotic cardiomyocytes.
Pol J Pathol 2002
PMID:Effect of melatonin on cytotoxic effects of daunorubicin on myocardium and on transplantable Morris hepatoma in rats. 1259 37

TP53 is the most frequently mutated gene in human cancer, with a predominance of missense mutations scattered over 200 codons. In many cancers, specific mutation patterns can be identified, which are shaped by site-specific mutagenesis and by biological selection. In tobacco-related cancers (lung, head and neck), organ-specific patterns are observed, with many mutations compatible with the ones experimentally induced by tobacco carcinogens. In several other cancers, such as squamous cell carcinoma of the oesophagus or hepatocellular carcinoma (HCC), mutation patterns show geographic variations between regions of high and low incidence, suggesting a role for region-specific risk factors. HCC from high-incidence regions showing also a high prevalence of a specific Ser-249 TP53 mutation is one of the most striking examples of a mutagen fingerprint. All such assessments are useful to generate clues on the mutagenic mechanisms involved in human cancer. Moreover, it has been shown that DNA retrieved from plasma can be successfully used for detection of TP53 mutations, which gives hope for earlier more accurate detection of human cancers.
Acta Biochim Pol 2003
PMID:TP53 and mutations in human cancer. 1267 64

The systemic inflammatory reaction (acute phase response) is induced by many noxious stimuli but in all cases the inflammatory cytokines, such as interleukin-1-beta (IL-1beta) and interleukin-6 (IL-6), are involved. Liver cell response to inflammation manifested by a characteristic change in the profile of synthesized plasma proteins (acute phase proteins) has been extensively studied. Here we describe a model system of cultured human hepatoma HepG2 cells stimulated with IL-1beta to evaluate the transcriptome induced by this cytokine during 24 h of treatment. By using differential display analysis we found IL-1beta-induced upregulation of several genes coding for cellular trafficking/motor proteins, proteins participating in the translation machinery or involved in posttranscription/posttranslation modifications, proteases, proteins involved in cellular metabolism, activity modulators, proteins of the cell cycle machinery and also some new proteins so far functionally not classified.
Acta Biochim Pol 2003
PMID:Complex analysis of genes involved in the inflammatory response: interleukin-1-induced differential transcriptome of cultured human hepatoma HepG2 cells. 1451 40

The aim of this work was to evaluate the efficiency of the antioxidant system in patients with primary hepatocellular carcinoma. Total antioxidant status (TAS), superdoxide dismutase (SOD) and catalase (CAT) activity was examined in liver primary cancers and in blood serum of patients before and after surgery. In comparison with healthy liver, very low activity of TAS was observed in liver cirrhosis and in primary cancer. High activity of TAS in the blood serum of patients before and after surgery was comparable with TAS activity in blood serum of healthy persons. The highest activity of SOD and CAT was observed in liver cirrhosis. The lowest activity was observed in liver primary cancer. Activity of SOD and CAT in the blood serum of patients before surgery was higher than in the blood serum of patients after surgery. The highest activity was observed in the blood serum of healthy persons. Obtained results shows, that the dysfunction of the defensive antioxidant mechanisms have characterised with not only local disturbances (in the tumour cells region), but also circuital ones (blood). Low levels of the activity of TAS, SOD and CAT in patients with primary hepatocellular carcinoma indicate to the distortion of the oxidant--antioxidant balance and the decrease of organism antioxidant system efficiency. These observations show at the participation of free radical processes in the tumour pathogenesis.
Pol Merkur Lekarski 2003 Aug
PMID:[Evaluation of antioxidant status in patients with primary hepatocellular carcinoma]. 1464 72

Hepatocellular carcinoma (HCC) and metastases from colorectal carcinoma are two most common malignant tumors affecting liver. Of all patients presenting with a malignant hepatic tumor, few are surgical candidates. Contraindications to hepatic resection include too many tumors, tumors in unresectable locations, insufficient hepatic reserve to tolerate resection, and other medical conditions that make the patient a poor surgical risk. A number of alternative therapies have been used for the treatment of malignant hepatic tumors. These include chemoembolization, ethanol injection therapy, and thermal ablation techniques. In this article are described chemoembolization and ethanol injection therapy.
Pol Merkur Lekarski 2004 Jan
PMID:[Chemoembolization and ethanol ablation of primary hepatic carcinoma and metastatic tumors]. 1507 29

Hepatocellular carcinoma (carcinoma hepatocellulare) kills about 1.25 million of people a year all over the world and makes 1.5% of all malignant neoplasms. Many factors play a role in etiology of hepatocellular cancer; the most important seem to be: hepatitis B and C viruses, alcohol and aflatoxin exposure. They all can cause hepatic cirrhosis. In present days, however, it is assumed that all diseases which lead to hepatic cirrhosis may be complicated by growth of primary liver cancer, but degree of risk of its development is various. Hepatocellular carcinoma grows in three macroscopic forms: nodular (multifocal), massive (unifocal) and diffusely infiltrative. Its varying microscopic pattern has resulted in separating four major types: trabecular, pseudoglandular, solid and scirrhous. Immunohistochemical analysis of hepatocellular carcinoma plays an important role in practical diagnosis. Metastatic tumors of the liver (breast, pancreas, kidney and adrenal gland cancers) are more common than primary ones. In routine histological examination they may imitate primary hepatic cancer. Precise diagnosis is of vital importance for therapy and prognosis.
Pol Merkur Lekarski 2004 Mar
PMID:[Pathogenesis and morphology of hepatocellular carcinoma]. 1519 Jun 13

Wistar and Buffalo rats of both sexes, aged 4 months, were divided into three groups: I which was given an intramuscular injection of 3 x 10(6) cells of Morris hepatoma (Buffalo males), II--subcutaneous injection of 3 x 10(4) cells of mammary gland carcinoma (Wistar females), III--intraperitoneal injection of 3 x 10(4) cells of Yoshid sarcoma (Wistar males). The animals were killed: in group I--19, group II--13 and in group III--6 days after tumor transplantation. Twenty four hours before euthanasia the rats were given 5-brome-2'-deoxyuridine (BRd-U) at a dose of 50 mg/kg body mass. The control group consisted of animals with tumour. They were not treated with BRd-U. Immunocytochemical reaction was performed on the sections of tumors, using monoclonal anti-BRd-U clone BU-33, Sigma. Computer measurements of tumor cells were carried out. There was a high similarity in morphological parameters between two kinds of cancer, and clear differences between them and Yoshid sarcoma. The main difference was noted in a twofold increase in the quantity of synthesised DNA in the nuclei of sarcoma cells. Immunocytochemical identification of tumor cells in phase S of the cell cycle with the use of monoclonal anti-BRd-U antibody is a precise and quick method of estimation of their proliferative potential.
Pol J Vet Sci 2004
PMID:Immunocytochemical identification of neoplastic cell clone in phase S of cell cycle in transplantable rat tumors. 1547 60


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