Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0019204 (hepatocellular carcinoma)
 71,386 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Shuttle plasmid containing HSV-tk gene and regulatory sequence of the afp gene was constructed and recombined with the right arm of adenovirus DNA. The recombinant adenovirus vector was named pAdrAFPTK. Meanwhile, an AdCMVTK was constructed as control in which the tk gene was controlled under CMV promoter. PCR and Southern blot analyses were used to identify positive plaques. Virus titer was about 1x10(15) pfu/L determined by plaque forming assay. The AFP-positive cells or AFP-negative cells were infected with AdCMVTK or AdrAFPTK and then treated with GCV, respectively. Cytotoxic effects were assayed with MTT method. The IC(50) of GCV for both HeLa cells or BRL-3A cells (both were AFP-negative cells) and HepG2 cells (AFP-positive cells) were 1.3 &mgr;mol/L, 2&mgr;mol/L and <1&mgr;mol/L, respectively, after infection with AdCMVTK (m.o.i.=100). However, in the cases of infection with AdrAFPTK (m.o.i.=100), IC(50) were 1 000 &mgr;mol/L, >1 000 &mgr;mol/L and <1&mgr;mol/L for HeLa cells, BRL-3A cells and HepG2 cells, respectively. Results showed that this vector possessed advantages of high title, high infectivity coming from adenovirus and the character of cell type-specificity gene expression. The AdrAFPTK/GCV system may become a new, potent and specific approach for the gene therapy of the primary hepatoma.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1998
PMID:Specific Expression of Suicide Gene in Liver Cancer Cells Mediated by Adenovirus. 1216 32

Two chimerical regulation sequences for gene expression, one (called ATrPS) harboring an enhancer of human alpha1-antitrypsin gene (AT) and a promoter and silencer(rPS)of rat afp gene, and another (called rAFP) consisting of enhancer III of rat afp gene and its rPS, were constructed, respectively. Then, two CAT expression vectors, rAFP-pCAT and ATrPS-pCAT, were constructed in which the cat reporter gene was put under the control of these elements, respectively. CAT activities could were detected in the AFP positive liver cancer cells and also in those liver cancer cells, liver cells or non-liver cells whose AFP were negative. Results showed that for both constructs, the CAT activities could be found in all AFP positive hepatoma cells, however, while this activity can not be detected in all AFP negative cells. Similar results were observed in primary cell cultures. The results showed that our regulation elements of gene expression really possessed cell-type specificity for AFP positive cells. The cell-type specificity remains when the length of rAFP was cut short as to 0.67 kb; and the activity seemed higher. These regulatory sequences of gene expression may be used in gene therapy for primary liver cancer.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1998
PMID:Construction of Two AFP-positive Hepatoma Cell-specific Gene Regulatory Sequences. 1216 38

We studied the activities of calcium-independent phosphatidylcholine-specific phospholipase C (PC-PLC) and the relationship between PC-PLC and gamma-GT in hepatoma cells. We noted that PC-PLC activity decreased significantly during hepatocarcinogenesis and the proliferation of CBRH-7919 cells, but increased significantly during differentiation. There was a close relationship between PC-PLC and gamma-GT activities. When CBRH-7919 cells were treated with exogenous PC-PLC, it took only 15 minutes for gamma-GT activity to decrease significantly and to the lowest level at 24 hours. However, gamma-GT activity in culture medium increased within 2 hours, then decreased afterward. We conclude that PC-PLC may regulate gamma-GT via certain pathways.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1998
PMID:Correlation of Calcium-independent Phosphatidylcholine-specific Phospholipase C with gamma-GT in Hepatoma Cells. 1217 8

Restin, a homologous protein of endostatin (62% homology), is the NC domain of collagen XV at C-terminal. The recombinant restin expressed in E. coli had the ability to suppress the proliferation of bovin aortic endothelial cells and cause apoptosis. In this report, mouse restin gene was fused with a sequence of human plasminogen signal peptide by PCR and cloned into eukaryotic expression vector pCDNA3. The plasmid containing restin gene was named pCDNAXV and was transfected into human hepatoma cell line Bel7404. Stable transfected clones were screened and expression of restin was confirmed by RT-PCR and Western blot. The proliferated cells were injected subcutaneusly into nude mice. The growth of tumors formed by cells transfected with restin gene was much slower than that of control group. These results indicated that the expressed restin in vivo could suppress the growth of tumor, and this suppression might be achieved by restraining angiogenesis since the restin had no effect on the proliferation of tumor cells. At the same time, this report provided a new method to investigate the effect of anti-angiogenetic proteins on the tumor growth.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2002 Sep
PMID:[Restin expressed in vivo suppresses the growth of tumors in nude mice]. 1219 58

5-Aminolevulinic acid (ALA) is a precursor to heme synthesis pathway and currently used to induce endogenous protoporphyrin IX (PpIX, a potent photosensitizer) for photodynamic therapy of cancer. ALA has, however, a limited ability to cross cellular membranes due to its low lipid solubility. The use of lipophilic ALA esters may increase cellular uptake, which results in an enhanced PpIX synthesis. In the present study, a comparison of ALA and its hexyl ester (He-ALA) was made in the QGY human hepatoma cell line with respect to PpIX production and its photocytotoxicity. The fluorescence emission spectrum of the cells incubated with He-ALA was identical to that of PpIX, indicating that He-ALA could induce PpIX in the cells. Fluorescence images demonstrated that the He-ALA induced PpIX was localized in the cytoplasm of the cells. Moreover, a similar amount of Pp IX was found in the cells incubated with 0.2 mmol/L He-ALA or 2 mmol/L ALA and a similar level of cell survival was reached following light exposure. These results suggest that He-ALA is much more efficient at producing PpIX and photocytotoxicity than ALA itself in the cells.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2002 Sep
PMID:Comparison of 5-aminolevulinic acid and its hexylester mediated photodynamic action on human hepatoma cells. 1219 71

To investigate the infection of HCV and the expression of HCV antigen (HCAg) in HCC, immunohistochemical protocol was performed on tumorous liver tissues from 40 patients with HCC to detect HCAg and hepatitis B virus surface antigen (HBsAg) simultaneously. The results showed that the positive rates for HCAg and HBsAg were 17.5% (7/40) and 70% (28/40) respectively. HBsAg and HCAg were simultaneously detected in six of 40 cases and only one case had HCAg positive alone in the liver tumorous tissue. The positive signals were localized in diffuse cytoplasm of hepatocytes and the positively stained cells were mainly distributed in local pattern. The results suggested that HCV infection did exist in some cases of HCC, so HCV might be a viral risk factor in addition to HBV in the genesis of HCC in China.
Hua Xi Yi Ke Da Xue Xue Bao 1999 Jun
PMID:[Immunohistochemical detection of hepatitis C virus antigen from hepatocellular carcinoma]. 1221 37

The effect of phorbol 12-myristate-13-acetate (PMA) on the hydrolysis of phosphatidylcholine (PC) in rat hepatoma cell line CRBH7919 has been studied. It was found that PMA stimulated PC hydrolysis in CRBH7919 cells in a dose-dependent manner after treatment for 15 min. The product of PC hydrolysis was choline, not phosphocholine. The activity of the membrane bound PC-specific phospholipase D (PC-PLD) was determined. The results showed that the activity of PC-PLD increased after 10 min with 100 nM PMA treatment, and reached a level 3.25 times the control after 30 min. The fact that the PC-PLD activation preceded the hydrolysis of PC, suggests that PC-PLD is involved in the PC hydrolysis into phosphatidic acid and choline in CRBH7919 cells in the presence of PMA.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1997
PMID:PMA Stimulated Hydrolysis of Phosphatidylcholine in CRBH7919 Cell and Its Enzymatic Basis. 1221 68

H7C is a HBV integrated fragment isolated from a human hepatocellular carcinoma, containing the promoter of preS2 and the C-terminal truncated preS/S open reading frame. We have studied the effect of the 3'-truncated preS/S on human proliferating cell nuclear antigen (PCNA) promoter by co-transfection of the expression plasmids. Result showed that the product, pKSH7C-Hpa I, which contained the intact H7C and the flanking cellular sequences, stimulated the expression from PCNA promoter dose-dependently, and its effect was 1-2 folds higher than that on SV40 promoter. However, two subclones, pKSH7C-XHX and pKSH7C-XbH, which would not express preS/S, showed no stimulatory effect. Furthermore, when if the -45 bp ATF-like site was mutated, the activation effect became diminished. This showed that the ATF-like site might be important in mediating the transactivating process. This is the first report of the effect of a HBV integrated fragment on the promoter of a replicating protein factor.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1997
PMID:Regulatory Effect of HBV Integrated Fragment on PCNA Promoter. 1221 96

This study was directed at the antitumor activity of the lysates prepared from CD3McAb activated killer cells. We separated the peripheral blood monocytes(PBMC) from normal adults. The PBMC were induced by monoclonal antibody specific for CD3(CD3McAb) and activated by rIL-2. The CD3McAb-activated killer cells (CD3AK) were smashed by ultrasonic wave, along with frozen and thawed three times, then the lysates were obtained by centrifugation. The lysates were tested for antitumor activity in vivo and in vitro. The results revealed that the inhibition rate of the lysates that acted on mice solid tumor hepatoma 22(H22) was 68.20% and the killing activity of the lysates on K562 and Raji were 83.32%, and 66.83% respectively. The results of this experiment suggested that the lysates prepared from CD3McAb-activated killer cells is one of probable agents that might apply to biotherapy for tumors.
Hua Xi Yi Ke Da Xue Xue Bao 2000 Mar
PMID:[Antitumor activity of the lysates prepared from anti-CD3 antibody activated killer cells]. 1250 11

EGR-1 gene promoter and CDglyTK gene were used to construct the pcDNA3-EGR-CDglyTK recombinant vector, in which CDglyTK gene expression was under the control of EGR-1 gene promoter. Cationic liposome LipofectAMINE was used to transfect plasmids into human hepatoma 7402 cell line. Subsequently, the transfected cells were treated with different doses of gamma-ray. Northern blot and Western blot showed that ionizing radiation can induce CDglyTK gene expression drived by EGR-1 promoter,in a dose-dependant manner. There was no ionizing radiation-inducible effect in pcDNA3-CMV-CDglyTK control group. Ionizing radiation also markedly enhanced the sensitivity of tumor cells transfected with pcDNA3-EGR-CDglyTK to prodrugs (GCV/5-FC) in tumor cell killing. The data indicated that EGR-1 promoter was inducible by ionizing radiation, whereas the CMV promoter was not. There was a synergetic effect between GCV and 5-FC. The cytotoxic effect of the suicide gene-ionizing radiation combination was stronger than suicide gene alone or ionizing radiation alone.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2003 Jan
PMID:[Ionizing radiation-regulated killing of human hepatoma cells by liposome-mediated CDglyTK gene delivery]. 1251 30


<< Previous 1 2 3 4 5 Next >>