Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0019204 (
hepatocellular carcinoma
)
71,386
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ser/Thr protein phosphatase 5 (
PPP5C
) has been reported to participate in tumor progression. However, its functional role in
hepatocellular carcinoma
(
HCC
) remains unknown yet. In this study, we firstly evaluated the expression levels of
PPP5C
in six
HCC
cell lines by real-time PCR and found that
PPP5C
was widely expressed in
HCC
cells. To explore the role of
PPP5C
in
HCC
cell growth, lentivirus-mediated short hairpin RNA (shRNA) was employed to silence
PPP5C
expression in HepG2 and Bel-7404 cells. The expression of
PPP5C
was significantly downregulated in
PPP5C
knockdown cells. Knockdown of
PPP5C
markedly suppressed the proliferation and colony formation ability of
HCC
cells. Moreover, cell cycle analysis showed that
PPP5C
depletion in HepG2 cells led to G0/G1 phase and G2/M phase arrest. We demonstrate for the first time that
PPP5C
is essential for growth of
HCC
cells, which suggests that inhibition of
PPP5C
by RNAi may be a potential therapeutic strategy for the treatment of
HCC
.
...
PMID:Knockdown of PPP5C inhibits growth of hepatocellular carcinoma cells in vitro. 2532 85
Serine/threonine protein phosphatase 5 (
PPP5C
) participates in multiple signaling pathways including cell cycle control and cell growth.
PPP5C
is involved in the progression of human breast cancer and
hepatocellular carcinoma
. However, its function in acute myelogenous leukemia (AML) remains unknown. In this study, we constructed a lentivirus system to knock down the expression level of
PPP5C
in leukemic cell line U937. Cell proliferation and cell cycles were assessed by MTT assay and flow cytometry respectively. Western blot was used to determine the level of caspase-3, PARP (poly ADP-ribose polymerase), CDK4 and CyclinD1. Knockdown of
PPP5C
suppressed the proliferation ability of U937 cells, and led to G0/G1 phase arrest, inducing cell apoptosis in U937 cells. The apoptosis of the U937 cells was associated with upregulating cleaved caspase-3 and PARP, and downregulating CDK4 and CyclinD1. In conclusions,
PPP5C
knockdown inhibits U937 cell proliferation and might be used as a potential therapeutic target for the treatment of leukemia.
...
PMID:Knockdown of protein phosphatase 5 (PPP5C) suppresses the growth of leukemic cell line U937. 2775 48
