Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0019204 (hepatocellular carcinoma)
 71,386 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Minimal deviation hepatoma 7288 C cells were cultured in Swim's medium containing 10% serum for 48 hr. The growth medium was replaced with serum free media containing different concentrations of [1-14C] eicosa-8,11,14-trienoic acid and the cells were incubated for 24 hr. Incorporation into cell lipids, oxidation to CO2, and desaturation to arachidonic acid were studied. The oxidation of the acid was very low. It was preferentially incorporated into the polar lipids of the cell. The incorporation depended on the number of cells and fatty acid concentration. Saturation of the cells with the acid was reached when 144.7 nmoles per mg of cellular protein were incorporated. The acid was desaturated readily to arachidonic acid. The nmoles of eicosatrienoic acid converted to arachidonic acid per mg of cellular protein were hyperbolic function of the acid incorporated. Maximal desaturation, 23 nmoles per mg of cellular protein, was reached when the cells were saturated with the acid. The calculations of the desaturation capacity and of the endogenous pool of eicosatrienoic acid available for desaturation in the cell are discussed.
 ...
PMID:Uptake and metabolism of exogenous eicosa-8,11,14-trienoic acid in minimal deviation hepatoma 7288 C cells. 17 60

Minimal Deviation Hepatoma 7288 C cells were cultured in confluent layer with labeled linoleic, alpha-linolenic, eicosa-8,11,14-trienoic and stearic acids. The cells in culture preserved their ability to convert stearic acid to oleic acid. They also synthesized arachidonic acid from linoleic acid or eicosa-8,11,14-trienoic acid. The conversion was very low with linoleic acid and high with eicosatrienoic acid. Eicosapentaenoic acid and other homologs of the alpha-linolenic acid family were synthesized from alpha-linolenic acid. The biosynthetic patterns were the cells was modified by the fatty acid composition of the media.
 ...
PMID:Biosynthesis of unsaturated fatty acids in cultured minimal deviations hepatoma 7288 C cells. 17 70

The effect of insulin on the oxidative desaturation of 1-14C palmitic acid to palmitoleic acid (delta 9 desaturase) 1-14C linoleic acid to alpha-linolenic acid (delta 6 desaturase) on rat liver microsomes and 1-14C eicosa-8,11,14-trienoic acid to arachidonic acid (delta 5 desaturase) on rat liver microsomes and hepatoma tissue culture (HTC) cells was studied. After 12 h of insulin injection, at a dose of 2.5-12.5 U/kg no change was found in delta 9 desaturation activity while delta 5 desaturation activity decreased. The conversion of linoleic to alpha-linolenic acid decreased when the amount of insulin injected was 5 U/kg or more. The effect of insulin (5 U/kg) on delta 9, delta 6 or delta 5 desaturation activity was tested from 1 to 12 h after the injection. The conversion of palmitic acid to palmitoleic acid showed no important changes along the time, while delta 5 desaturation activity decreased at all the times tested. delta 6 Desaturation activity showed a slight increase after 1 h of insulin treatment and then decreased significantly up to the end of the experiment. The addition of 400 mU/ml or more of insulin to the incubation medium of HTC cells produced a significant decrease on the conversion of eicosatrienoic acid to arachidonic acid. The effect of insulin on fatty acid desaturation activity of liver microsomes of normal rats differs from that of diabetic rats. The role of this hormone in relation to other hormones, carbohydrate metabolism and lipid biosynthesis on the activity of the desaturases was discussed.
 ...
PMID:Effect of insulin on the oxidative desaturation of fatty acids in non-diabetic rats and in isolated liver cells. 287 Jun 4

The effect of dexamethasone on the incorporation and conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid in isolated hepatocytes and in hepatoma tissue culture (HTC) cells was studied. In both kinds of cells, no changes in the exogenous acid incorporation were found when the hormone was added to the incubation media at 0.1 or 0.2 mM concentration, while the biosynthesis of arachidonic acid was significantly depressed. The effect on the biosynthesis was faster in isolated normal liver cells (60 min) than in tumoral cells (120 min) and reached an inhibition of ca. 50% after 3 hr of treatment. The addition of cycloheximide (10(-6) M) also caused a marked decrease in the biosynthesis of this polyunsaturated fatty acid, but when dexamethasone was added to the media simultaneously with cycloheximide, a synergistic action was not observed. The results obtained show that protein synthesis would be involved in the modulation of the biosynthesis of arachidonic acid by glucocorticoids. The changes in the delta 5 desaturation of labeled 20:3 omega 6 to arachidonic acid correlated with changes in the fatty acid composition in isolated cells.
 ...
PMID:Dexamethasone blocks arachidonate biosynthesis in isolated hepatocytes and cultured hepatoma cells. 308

The incorporation and conversion of eicosa-8,11,14-trienoic acid to arachidonic acid were studied in HTC cells (7288 c hepatoma cells) and isolated hepatocytes of rat. The cells were incubated at different concentrations of the acid during 0 to 6 hours. Both kinds of cells were able to take up the acid. However, the HTC cells have a greater avidity for the eicosatrienoic acid than normal liver cells. The incorporation of the acid modified the fatty acid composition of the cells and caused a decrease in the amount of saturated and monoenoic acid. In both cells eicosatrienoic acid was converted to arachidonic acid. However, in HTC cells arachidonic acid level was low and not correlated with the amount of the eicosatrienoic acid incorporated. This fact is apparently not due to an impairment in delta 5 desaturation activity since this enzyme is active in both cells. It would be possible that arachidonic acid production in malignant cells would be also interrupted in another metabolic pathway after delta 6 desaturation step. The strikingly low amount of arachidonic acid biosynthesized in HTC cells compared to normal hepatocytes could be interpreted as a consequence of a lower availability of eicosatrienoic acid for the microsomal desaturation system in malignant cells, in addition to the low delta 6 desaturase activity.
 ...
PMID:Uptake and metabolism of eicosa-8, 11, 14-trienoic acid in normal hepatocytes and HTC cells. 392 May 2

The effect of different carbon sources on delta 5 desaturation activity in Minimal Deviation Hepatoma 7288 c (HTC) cells was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in S-77 medium (fed cells). This activity was not modified if fasted cells were refed with glucose. However, the activity decreased significantly by the addition of lactalbumin hydrolysate to fasted cells. This effect can not be attributed to individual aminoacids since the addition of them to an aminoacid free medium produced no changes on delta 5 desaturation activity. The incubation of HTC cells with S-77 medium supplemented with glucose, pyruvate or glucose plus pyruvate, showed similar delta 5 desaturation activity to those cells incubated in S-77 medium alone.
 ...
PMID:Effect of different carbon sources on delta 5 desaturation activity of HTC cells. 681 76

The effect of fasting on delta 5 desaturation activity in Minimal Deviation Hepatoma 7288c (HTC) cells and in rat liver microsomes was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C] eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in complete medium (fed cells). This effect was also shown in rat liver microsomes of rats fasted for 12, 24 and 48 hr. Fasting produced a decrease of the amount of ethanolamine fraction in HTC cells together with a significant increase in the specific activity of the same fraction. Fasting also modified the fatty acid composition of HTC cells and rat liver microsomes. There was observed a relative increase of 18:2 omega 6 acid. The importance of the difference in fatty acid and lipid composition among fasted and fed HTC cells and rat liver microsomes on delta 5 desaturation activity was discussed.
 ...
PMID:Effect of fasting on delta 5 desaturation activity in rat liver microsomes and HTC cells. 746 29