Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0019204 (hepatocellular carcinoma)
 71,386 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Long-dan-tan (Chinese name) is one of the most common herbal medicines used by Chinese people with chronic liver disease. Accumulated anecdotal evidence suggests that Long-dan-tan may show a beneficial effect in patients with hepatocellular carcinoma. Long-dan-tan is made from five plants: Gentiana root, Scutellaria root, Gardenia fruit, Alisma rhizome, and Bupleurum root. In this study, we have examined the cytotoxic effects of the five major ingredients isolated from the above plants, i.e. gentiopicroside, baicalein, geniposide, alisol B acetate and saikosaponin-d, respectively, on human hepatoma Hep3B cells. Annexin V immunofluorescence detection, DNA fragmentation assays and FACScan analysis of propidium iodide-staining cells showed that gentiopicroside, baicalein, and geniposide had little effect, whereas alisol B acetate and saikosaponin-d profoundly induced apoptosis in Hep3B cells. Alisol B acetate, but not saikosaponin-d, induced G2/M arrest of the cell cycle as well as a significant increase in caspase-3 activity. Interestingly, baicalein by itself induced an increase in H(2)O(2) generation and the subsequent NF-kappaB activation; furthermore, it effectively inhibited the transforming growth factor-beta(1) (TGF-beta(1))-induced caspase-3 activation and cell apoptosis. We suggest that alisol B acetate and saikosaponin-d induced cell apoptosis through the caspase-3-dependent and -independent pathways, respectively. Instead of inducing apoptosis, baicalein inhibits TGF-beta(1)-induced apoptosis via increase in cellular H(2)O(2) formation and NF-kappaB activation in human hepatoma Hep3B cells.
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PMID:Pharmacological evaluation of several major ingredients of Chinese herbal medicines in human hepatoma Hep3B cells. 1290 91

The cytotoxic effect of a herbal medicine Shosaiko-to (TJ-9) was examined by the MTT assay on 7 human lung cancer cell lines (4 non-small cell carcinomas, 3 small cell carcinomas) and on 5 hepatocellular carcinoma cell lines. TJ-9 showed a dose-dependent cytotoxicity in all cell lines except one (SBC-5). Of the seven herbs in TJ-9, Scutellaria root showed the strongest cytotoxicity followed by the Glycyrrhiza root. Among baicalin, baicalein and wogonin from the Scutellaria root, cytotoxicity was observed only with baicalin. The SBC-5 cell line which was resistant to TJ-9 showed a lesser sensitivity to both Scutellaria root and baicalin. TJ-9 showed almost equal cytotoxicity in cisplatin (CDDP)-sensitive PC-10 and CDDP-resistant SBC-4 cell lines, and in H69 and H69/CDDP cell lines. TJ-9, Scutellaria root and baicalin were all less cytotoxic for human lymphocytes and bone marrow cells than for a lung cancer cell line of SBC-4. These results suggest that TJ-9 and its components may be useful anticancer agents for the treatment of lung cancer.
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PMID:Cytotoxic effect of herbal medicine sho-saiko-to on human lung-cancer cell-lines in-vitro. 2159 96