Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0019204 (hepatocellular carcinoma)
 71,386 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Four Aroclor reference materials, cleaned-up extracts of 2 yusho rice oil samples, and cleaned-up extracts of 3 fish samples containing polychlorinated biphenyl (PCB) residues were tested for their ability to induce aryl hydrocarbon hydroxylase (AHH) activity in a rat hepatoma cell line. Before the AHH bioassay, the samples were fractionated by a Florisil column chromatographic method. All samples contained about 1000 micrograms PCBs before Florisil column chromatography. The first Florisil eluate contains about 95% of the PCBs in a typical Aroclor, and the second contains the more polar or adsorbent PCB congeners. In this study, the first eluate for all samples produced no quantifiable AHH activity. The second Florisil eluates of both Aroclors 1242 and 1248 induced AHH activity, whereas these eluates of both Aroclors 1254 and 1260 did not. This difference may be due to the presence in Aroclors 1242 and 1248 of 3,3',4,4'-tetrachlorobiphenyl, which has not been detected in Aroclors 1254 and 1260. The second Florisil eluates of the fish samples induced somewhat less AHH activity than did Aroclor 1242 or 1248. The second Florisil eluates of the PCB residues from yusho rice oil samples induced significantly greater AHH activity than these eluates of either Aroclor 1242 or 1248, perhaps because yusho rice oil contains a greater amount of polychlorinated dibenzofurans than PCB commercial mixtures on a PCB equivalent basis.
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PMID:Induction of aryl hydrocarbon hydroxylase activity in cell cultures by aroclors, residues from yusho oil samples, and polychlorinated biphenyl residues from fish samples. 681 43

Blue mussels (Mytilus edulis) collected from 34 locations along the south and east coast of Korea were analyzed for polychlorinated biphenyls (PCBs) and organochlorine (OC) pesticides. Maximum concentrations of PCBs and total OC pesticides were 98.5 and 20.5 ng/g, wet weight, respectively. Extracts were fractionated by Florisil chromatography and each fraction was screened for dioxin-like activity in vitro, using recombinant rat hepatoma cells (H4IIE-luc). Fraction 2 (F-2), which contained hexachlorocyclohexanes, chlordanes, p,p'-DDD, and p,p'-DDT, generally elicited significant dioxin-like activity compared to control, whereas Fraction 1 (F-1), which contained PCBs, p,p'-DDE, and hexachlorobenzene, did not. The greatest magnitude of dioxin-like response observed was 44% of the maximum response elicited by a 2,000 pM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) standard. The relatively low magnitudes of dioxin-like response observed for F-1 samples were consistent with the relatively low PCB concentrations. At concentrations equal to the maximum observed in the mussel samples, neither individual OC pesticides nor a mixture of OC pesticides yielded a significant dioxin-like response in the H4IIE-luc assay. Thus, the concentrations of OC pesticides in F-2 did not appear to have accounted for the dioxin-like activity observed. This suggests the presence of unidentified and/or unknown, acid-stable, dioxin-like compounds in F-2. This study suggests that in vitro bioassays are useful in assessing the contamination of mussels collected from coastal marine locations.
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PMID:Instrumental and bioanalytical measures of persistent organochlorines in blue mussel (Mytilus edulis) from Korean coastal waters. 1094 87