Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0019204 (hepatocellular carcinoma)
 71,386 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Substrate-attached critical-point-dried cells cleave along the level of the substrate-adherent membrane if removed by means of adhesive tape. The remaining membrane fragments on grids can be visualized three-dimensionally by means of stereo transmission electron microscopy. Attachment of cells may be achieved by active spreading of the cell, or artificially by poly-L-lysine adherence of prefixed cells. In 11 different cell types a filamentous network appears to remain associated with the cytoplasmic face of the membrane. In one hepatoma cell type virtually no filamentous network could be detected. Two general network morphologies are described: the hepatocytic network and the lymphoid network. Since no correspondence could be found between cytoplasmic structure and the structure of the membrane-associated network, and since cells generally cleave along the level of this network, excluding cell organelles, we conclude that it comprises a distinct structural system, analogous to the membrane skeleton of the red cell membrane.
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PMID:Plasma membrane-associate filament systems in cultured cells visualized by dry-cleaving. 636 31

In cytotoxicity and indirect immunofluorescence tests an antiserum to ACA-1 (activated cell antigen) reacted with 58-100% of actively proliferating cells from tumors of lymphoid (EL-4 T lymphoma, MOPC 104E plasmacytoma) and nonlymphoid origin (AH-22 hepatoma, Sa-1 and MCh-11 sarcomas, F2 mammary cancer). Absorption of anti-ACA-1 serum with tumor cells sharply reduced its activity both against the cells of all these neoplasms and against normal activated T and B lymphocytes. Absorption with proliferating murine cells from the brain of embryos and the retina of neonates or with similar (nonproliferating) cells from adult mice did not affect the activity of the antiserum. It is concluded that ACA-1 is expressed on actively proliferating cells of the tumors studied.
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PMID:Expression of the differentiation antigen of activated T and B lymphocytes (ACA-1) on cells of lymphoid and nonlymphoid tumors. 642 51

Human alpha 1-antitrypsin ( alpha-1-AT;Pi) production was analyzed in 11 primary mouse hepatoma-human lymphoid cell hybrids and in 14 secondary rat hepatoma-human fetal liver fibroblast hybrids. The presence of human alpha-1-AT was determined by Laurell immunoelectrophoresis of concentrated and isotopically labeled supernatant medium. Human alpha-1-AT production segregated in the mouse-human hybrids concordantly with human purine nucleoside phosphorylase and with chromosome 14. All rat-human hybrids that were alpha-1-AT positive were also positive for human purine nucleoside phosphorylase and chromosome 14. Our study demonstrated the usefulness of rodent hepatoma cell hybrids for mapping human liver-specific genes because differentiated functions are expressed despite the fact that the human parental cells did not express these functions. Our study also showed that human alpha-1-AT gene product can be processed for secretion in the rodent hepatoma cellular environment. The mouse-human hybrids showed that no other human chromosome carries genes necessary for processing or secretion of human alpha-1-AT in the hybrid cell milieu.
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PMID:Assignment of human alpha 1-antitrypsin to chromosome 14 by somatic cell hybrid analysis. 680 64

The ability of indium-111 (111In)-oxine-labeled syngeneic lymphocytes to migrate normally and their suitability for imaging both normal lymphoid structures and those with metastatic disease were assessed. Sixty-two ACI rats were studied, 34 of which received injections in the left foot pad with 1 X 10(6) syngeneic H-4-II-E hepatoma cells nine to 44 days before imaging. Most animals were bearing palpable tumors when imaged. In 53 experiments, 1-6 X 10(8) 111In-oxine-labeled lymphocytes with a labeling concentration of 5-80 microCi/10(8) cells were injected intravenously. Gamma camera images were obtained 22 hours later. After the last image, the animals were killed. The lymph nodes, liver, spleen, lungs, and left femur were dissected, and the recovered radioactivity was determined in a gamma well counter. Lymph nodes could be partially or completely visualized in 70% of the animals (15 tumor-bearing and 16 normal out of 44 technically satisfactory experiments). Large metastatic nodes were seen clearly. Lymphocytes labeled with 111In-oxine exhibited a normal migration from blood to lymph nodes.
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PMID:Imaging of lymphoid structures with indium-111-labeled lymphocytes. 683 36

Amongst 1,200 leukemie children treated between 1958 and 1971, 60 are in complete remission for more than 10 years and 100 for more than 7 years. There were 96 acute lymphoid and 4 acute myeloid leukemias. Ten patients who have relapsed in the past have not done so lately. The F/M sex ratio is 1.5. Poor prognostic features were initially absent in 2/3 of cases. In 1/3 there was associated hyperleucocytosis and/or tumours. 93 children are in remission, their treatment having been stopped for 1 to 12 years. Five children relapsed and 4 are in a second remission for more than 2 years. Two children died in remission: one from a hepatocarcinoma and one from cardiac failure. These patients have been shown to have the following: 1) normal growth; 2) normal puberty: 8 patients have been able to reproduce, giving 10 children, one with multiple malformations; 3) school achievement and later socioprofessional behaviour has been normal. The patients have often sought a medical or paramedical career. Sequelae are minimal, psychological problems being minimal in the child. With the protocols used, mean remission curve shows a plateau after 9 years and complete definitive care is achieved in 92 per cent of patients surviving at 7 years. The very distant future outlook is not known. No other malignant haematological disease has occurred but one child died from a carcinoma.
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PMID:[Acute leukemia in childhood: present status of 100 cases after 7 years of complete remission (author's transl)]. 693 27

Adoptive immunity against a syngeneic hepatoma (line-10) of Sewall-Wright inbred strain 2 guinea pigs was analyzed by a two-dimensional titration of iv transferred immune lymphoid cells versus intradermal tumor challenges. Tumor resistance increased exponentially as a function of the number of immune lymphoid cells transferred. Within the tumor challenge doses analyzed, suppression of tumor growth mediated by the transferred immune lymphoid cells appeared to be independent of the primary immune response in the recipient. Quantitatively, rejection of a given number of tumor cells reflected the number of transferred immune cells and was independent of the presence of the same tumor at other skin sites. There was no evidence indicating that transferred immune cells were attracted specifically to the tumor inoculation site. The number of tumor cells that could be rejected at a skin site by adoptive immunity was greater than the estimated number of immune lymphoid cells present at the challenge site.
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PMID:Mechanisms of immunologic eradication of a syngeneic guinea pig tumor. I. Quantitative analysis of adoptive immunity. 695 Oct 81

The growth of syngeneic hepatoma H-2-73 in adult (CBA x C57BL/6j) F1 mice leads to the development of leukemoid response that is manifested by leukocytosis, splenomegaly and a considerable increase in the content of lymphoid cells in the spleen. Meanwhile the newborn recipients of the tumor do not develop leukemoid response but manifest physiological changes in the hemopoietic tissue normally occurring within the first weeks after birth. The sensitized cells of the spleen of the adult tumor-bearing mice enhance tumor growth in the newborn recipients and inhibit it in the adult mice. Spleen cells of normal adult donors do not affect tumor growth in the newborn recipients. The data obtained suggest that the opposite effect of the transferred sensitized spleen cells from the adult animals on tumor growth in the newborn and adult mice is related to the age-associated features of the immune system of recipient mice.
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PMID:[Characteristics of hematopoietic system reactions of newborn and adult mice to the growth of syngeneic hepatoma H-2-73]. 711 45

The cross-reactivity of progestins for glucocorticoid receptors was exploited to photoaffinity label glucocorticoid receptors from cultured rat hepatoma (HTC) and mouse lymphoma (S49) cell cytosol. The synthetic progestin, 17 alpha, 21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione (R5020), rapidly forms covalent bonds with protein upon irradiation of either cytosol with 350 nm light. Polyacrylamide gel electrophoresis under denaturing conditions reveals a single band photolabeled by R5020 that is not observed when excess nonradioactive dexamethasone is included in the incubation. This protein band corresponds to a molecular weight of about 87,000 in both HTC and S49 cell cytosol; it is entirely absent in cytosol from glucocorticoid-resistant S49(r-) cells which lack receptor-binding activity. Another steroid-resistant mutant, S49 (nti), which exhibits normal levels of steroid-binding activity but increased binding of receptor-steroid complexes by the nucleus, yields a receptor which, when photolabeled, has an apparent molecular weight of only 39,000. These results demonstrate that glucocorticoid receptors can be photoaffinity-labeled; the data are consistent with the notion that the binding form of the receptor consists of a single polypeptide chain, Mr = 87,000, in two different species, rat and mouse, and in cells of either hepatic or lymphoid origin. The data also suggest that the lesion in the steroid-resistant S49 (nti) lymphoma cell line is a mutation of the structural gene for the glucocorticoid receptor which results in the synthesis of a truncated protein.
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PMID:Photoaffinity labeling of glucocorticoid receptors. 719 70

Pigmented subtetraploid subhexaploid mouse melanoma cells were fused with a range of different cell types. Expression of pigment formation appeared to be dependent on the phenotype of the non-melanoma parent cell, so that hybrids with lymphoid cells or chick embryo erythrocytes produced pigment, but hybrids between fibroblasts or epithelial rat hepatoma cells did not. The results were independent of gene dosage of either parent cell. gamma-irradiation of suppressing partner cells prior to fusion caused progressive increase in pigmentation with increasing dose of radiation. Cybrids between cytoplasts of suppressing fibroblasts and melanoma cells were pigmented.
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PMID:Persistence, suppression and re-expression of pigment formation in somatic cell hybrids between mouse melanoma cells and non-melanoma cells. 726 79

A morphological investigation was carried out to study the pathological features of liver cirrhosis caused by hepatitis C virus (HCV) infection. The materials consisted of liver specimens taken from 47 cases of anti-HCV antibody-positive liver cirrhosis (37 by surgery for hepatocellular carcinoma and 10 by autopsy), and from 21 cases of hepatitis B surface antigen-positive liver cirrhosis as the control. Liver specimens containing more than 10 regenerative nodules were examined. In addition, a histometric study was conducted to determine the degree of fibrosis and the size of regenerative nodule using a computer image-analysis system. The results showed that the histological characteristics of HCV antibody-positive liver cirrhosis are: (i) broadly expanded fibrous septa and small regenerative nodules; (ii) relatively strong inflammatory reaction and prominent lymphoid aggretation in the fibrous septum; and (iii) mild regenerative activity of the liver parenchyma, and infrequent liver cell dysplasia. These findings may facilitate better understanding of the pathology of HCV antibody-positive liver cirrhosis and more accurate pathological diagnosis by needle biopsy.
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PMID:Pathomorphological study of HCV antibody-positive liver cirrhosis. 753 51


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