Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0019163 (hepatitis B)
 38,309 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Pleural effusion presentation of posttransplant lymphoproliferative disorder (PTLD) is relatively uncommon. Most examples of effusion-based PTLD have been secondary to widespread solid organ involvement, and are associated with an aggressive clinical course. We report on a case of primary effusion PTLD in a 70-yr-old male liver transplant recipient with a history of hepatitis B infection. Cytomorphologically, the pleural fluid specimen showed a monomorphous population of intermediate to large-sized transformed lymphoid cells, with irregular multilobated nuclear contours and readily identifiable mitotic figures. Flow cytometric immunophenotypic studies revealed a CD5-negative, CD10-negative, lambda immunoglobulin light chain-positive, monoclonal B-lymphocyte (CD19-positive/CD20-positive) population. The immunocytochemical stain for CD30 antigen was negative. In situ hybridization study for Epstein-Barr virus (EBV) early RNA (EBER) and Southern blot analysis for EBV terminal repeat sequences were both positive. Southern blot analysis for human herpes virus-8 (HHV-8) was negative. No solid-organ PTLD was identified, and the cytologic results supported the diagnosis of primary effusion PTLD. Immunosuppression was decreased, and 8 mo following the diagnosis of pleural fluid PTLD, the patient was stable and his pleural effusion had markedly diminished. Recognition of primary effusion PTLD and its distinction from PTLD secondarily involving the body fluids and from other lymphomas is important, since the behavior and prognosis appear different.
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PMID:Primary pleural effusion posttransplant lymphoproliferative disorder: Distinction from secondary involvement and effusion lymphoma. 1146 13

In acetate buffer solution, the reaction of H2O2 with KI was catalyzed by horseradish peroxidase (HRP) to form I3-. The I3- combined respectively with rhodamine S(RhS), rhodamine 6G(Rh6G), rhodamine B(RhB) and butyl-rhodamine B(b-RhB) to form RhS-I3, Rh6G-I3, RhB-I3 and b-RhB-I3 association particles, resulting in the fluorescence quenching at 580, 580, 554 and 554 nm, respectively. The effect of pH value, rhodamine dye concentration, KI concentration, H2O2 concentration, reaction temperature and time on the fluorescence quenching intensity (deltaF) of the four catalytic systems was considered respectively. For the RhS, Rh6G, RhB and b-RhB catalytic systems, pH 4.6-3.2 x 10(-5) mol x L(-1) RhS-4 x 10(-3) mol x L(-1) KI-1.30 x 10(-5) mol x L(-1) H2O2-25 degrees C-20 min, 4.8-2.4 x 10(-5) mol x L(-1) Rh6G-4 x 10(-3) mol x L(-1) KI-2.59 x 10(-5) mol x L(-1) H2O2-25 degrees C-20 min, 4.6-1.6 x 10(-5) mol x L(-1) RhB-4 x 10(-3) mol x L(-1) KI-2.16 x 10(-5) mol x L(-1) H2O2-25 degrees C-20 min, and 4.6-1.6 x 10(-5) mol x L(-1) b-RhB-4 X 10(-3) mol x L(-1) KI-3.02 x 10(-5) mol x L(-1) H2O2-25 degrees C-20 min were chosen for use respectively. Under the optimal conditions, the HRP linear range was 8-6 400 pg x mL(-1) for the RhS catalytic system, 40-4 000 pg x mL(-1) for the Rh6G catalytic system, 32-3 200 pg x mL(-1) for the RhB catalytic system and 40-6 400 pg x mL(-1) for the b-RhB catalytic system, with a detection limit of 3.2, 3.0, 2.4 and 3.7 pg x mL(-1) HRP, respectively. The regress equation of the four catalytic systems was deltaF = 0.061 1c+39.6, deltaF = 0.047 2c+50.4, deltaF = 0.138 6c+34.2 and deltaF = 0.026 25c+36.72, with a correlation coefficient of 0.997 9, 0.999 0, 0.997 3 and 0.996 9, respectively. The RhS catalytic system was most sensitive, and was chosen for the determination of HRP. The influence of foreign substance on the RhS assay of 3.5 ng x mL(-1) HRP was examined, with a relative error of +/- 10%. A 3000-times L-glutamic acid, L-lysine, Ca2+, Mg2+, Cu2+, Fe3+, Zn2+ and vitamin B6, 1000-times HAS etc did not interfere with the assay. This showed that the assay has good selectivity. The RhS fluorescence quenching assay was applied to the determination of HRP in the solution of hepatitis B surface antibody labeling HRP, with satisfactory results.
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PMID:[Fluorescence quenching assay of ultratrace horseradish peroxidase using rhodamine dye]. 1945 17