Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0019163 (
hepatitis B
)
38,309
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Although the biological importance of
hepatitis B
virus X protein (HBX) in the life cycle of
hepatitis B
virus has been well established, the cellular and molecular basis of its function remains largely undefined. Despite the association of multiple activities with HBX, none of them appear to provide a unifying hypothesis regarding the true biological function of HBX. Identification and characterization of cellular targets of HBX remain an essential goal in the elucidation of the molecular mechanisms of HBX. Using the Saccharomyces cerevisiae two-hybrid system, we have identified and characterized a novel subunit of the proteasome complex (
XAPC7
) that interacts specifically with HBX. We also showed that HBX binds specifically to
XAPC7
in vitro. Mutagenesis studies have defined the domains of interaction to be critical for the function of HBX. Furthermore, overexpression of
XAPC7
appeared to activate transcription by itself and antisense expression of
XAPC7
was able to block transactivation by HBX. Therefore, the proteasome complex is possibly a functional target of HBX in cells.
...
PMID:Proteasome complex as a potential cellular target of hepatitis B virus X protein. 876 72
Although the biological importance of
hepatitis B
virus X protein (HBX) in the life cycle of
hepatitis B
virus has been well established, the cellular and molecular basis of its function remains largely undefined. Despite the association of multiple activities with HBX, none of them appear to provide a unifying hypothesis regarding the true biological function of HBX. Identification and characterization of cellular targets of HBX remain an essential goal in the elucidation of the molecular mechanisms of HBX. Using the Saccharomyces cerevisiae two-hybrid system, we have identified and characterized a novel subunit of the proteasome complex (
XAPC7
) that interacts specifically with HBX. We also showed that HBX binds specifically to
XAPC7
in vitro. Mutagenesis studies have defined the domains of interaction to be critical for the function of HBX. Furthermore, overexpression of
XAPC7
appeared to activate transcription by itself and antisense expression of
XAPC7
was able to block transactivation by HBX. Therefore, the proteasome complex is possibly a functional target of HBX in cells.
...
PMID:The hepatitis B virus X protein: the quest for a role in viral replication and pathogenesis. 902 71
