Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0019163 (hepatitis B)
 38,309 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Background/aims: GAPD has been exhaustively investigated as a key cytosolic enzyme in glycolysis. In recent years GAPD has also been implicated in many cellular activities unrelated to glycolysis. However, although various functions have been ascribed to GAPD from rabbit muscle, human blood and rat tissues, no information is available on human liver GAPD. We have recently demonstrated that, as a cellular kinase, GAPD might interfere in the life-cycle of hepatitis B virus. We therefore investigated the enzymatic activities and subcellular localization of GAPD in normal human liver. Methods: GAPD and hepatocyte membranes were isolated from human liver homogenates to study the subcellular localization and enzymatic activities of GADP (kinase and ADP-ribosyltransferase). Results: (i) GAPD was recovered from the plasma-membrane-enriched fraction, in internal membranes, and in the cytosol; (ii) GAPD could be phosphorylated, a phenomenon inhibited by both GAP and NADH; and (iii) GAPD exhibits ADP-ribosyltransferase activity, which is stimulated by nitric oxide in a concentration-dependent manner. Conclusions: Human liver GAPD may play significant biological roles in addition to glycolysis, especially in signal transduction and in intracellular processes possibly involved in HBV infection.
 ...
PMID:Protein kinase and NO-stimulated ADP-ribosyltransferase activities associated with glyceraldehyde-3-phosphate dehydrogenase isolated from human liver. 1180 36

Breast cancer is one of the most common malignant cancers among women and a major clinical obstacle. Although studies have reported the abnormal expression of SIRT7 in breast cancer, whether the function of SIRT7 regulates the expression of long noncoding RNAs (lncRNAs) in breast cancer remains unknown. We aimed to determine the differential expressions of mRNAs and lncRNAs associated with SIRT7 and understand the regulatory mechanism of SIRT7 in breast cancer. RNA sequencing was performed to explore the transcriptome in MDA-MB-231 cells after SIRT7 depletion, and a total of 50,634 different transcripts were identified. In comparison with the negative control, siSIRT7 groups showed 240 differentially expressed mRNAs and 26 differentially expressed lncRNAs. Gene ontology analysis revealed that the differentially expressed mRNAs mainly regulated DNA replication, CXCR chemokine receptor binding, and maturation of large subunit rRNA from tricistronic rRNA transcript, nucleoplasm, mitochondrion, and NAD+ ADP-ribosyltransferase activity. Kyoto Encyclopedia of Genes and Genomes analysis showed that the differentially expressed mRNAs were mainly involved in pathways associated with MAPK signaling pathway, tumor necrosis factor signaling pathway, hepatitis B, and cancer. Moreover, the target genes of the differentially expressed lncRNAs mainly regulated the carboxylic acid metabolic processes and were involved in glycolysis pathway. The mRNA-lncRNA coexpression network comprised 186 mRNAs and 23 lncRNAs. Our results provide essential data regarding differentially expressed lncRNAs and mRNAs after the depletion of SIRT7 in breast cancer cells, which may be useful to elucidate the role of SIRT7 in breast cancer development.
 ...
PMID:Long noncoding RNA and mRNA profiling in MDA-MB-231 cells following RNAi-mediated knockdown of SIRT7. 2912 10