Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0019163 (hepatitis B)
 38,309 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Sensitive immunoassays are essential for establishing the efficacy of recombinant vaccines to hepatitis B virus (HBV). These experimental vaccines include the PreS2 and S domains of the HBV envelope protein. To facilitate measurement of antibody against HBV PreS2, we employed the immuno-ligand assay with silicon sensor-based detection. Labeling of immune reagents with the haptens biotin and fluorescein allows adaptation to the immunofiltration light addressable potentiometric sensor (LAPS) system. A biotinylated monoclonal anti-PreS2 antibody and anti-PreS2 in clinical serum samples competitively bind in liquid phase to a fluorescein labeled PreS2 + S antigen. Streptavidin mediates the immobilization on biotinylated nitrocellulose membranes. Fluorescein mediates binding of an anti-fluorescein urease conjugate to the immune complex. Urease serves as the signal-generating component which subsequently is measured in the LAPS reader. In comparison to a competitive RIA, the immuno-ligand assay demonstrated a four-fold improved sensitivity using a smaller sample volume. The higher sensitivity resulted in earlier detection of seroconversion during a clinical vaccine study.
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PMID:Detection of antibody to the PreS2 sequence of the hepatitis B virus envelope protein using an immuno-ligand assay with a silicon sensor detection system. 176 51

Starting from the quantification of the specific lesions for chronic hepatitis B and C, our study focused on (i) the correspondence between the necroinflammatory activity and the fibrosis stage ascertained through the Ishak scoring system, (ii) the classification overlaps and differences of Ishak vs. METAVIR score. The study group consisted of 202 cases with chronic hepatitis B and 751 cases with chronic hepatitis C, diagnosed based on liver biopsies. The fragments of hepatic tissue were routinely processed and stained with Hematoxylin-Eosin, trichrome Szekely, Gordon-Sweet silver impregnation, and Periodic Acid-Schiff. A semiquantitative evaluation was performed using the Ishak (for hepatitis B and C) and the METAVIR (for hepatitis C) scoring systems. Our results revealed that the comparison between hepatitis B and C, based on the necroinflammatory activity and fibrosis, is able to offer through the numeric values of the Ishak scoring system accurate proofs, which support the aggressivity of hepatitis C, because it develops fibrosis more quickly, even on the background of mild necroinflammatory activity. Also, our data showed that the necroinflammatory activity and the fibrosis are not processes which progress in a consistent pattern. The application of the METAVIR scoring system for the cases with chronic hepatitis C confirmed that there is not a direct correlation between necroinflammation and fibrosis. The Ishak scoring system provides through the wide range of numeric values attributed for the evaluation of necroinflammatory activity and fibrosis far more precise criteria for the appraisal of the degree of damage to the hepatic parenchyma at the time of the diagnosis. Supplementary, the METAVIR scoring system allows for the hepatitis C an assessment of the entire histologic activity, including the interface hepatitis and the associated lobular necrosis components. The scoring systems have unavoidably strengths and weaknesses, but the choice of a specific one must reflect the consensus between the pathologists and the clinicians, relying on their experience.
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PMID:Operational scores in the diagnosis of chronic hepatitis. A semi-quantitative assessment. 2239 4

Hepatitis B virus (HBV) is a major cause of chronic liver disease and an important public health issue. This study was conducted with the objective of evaluating the histological features in patients infected with precore and wild-type HBV infection; compare the histological activity and fibrosis stage and early treatment of HBeAg negative hepatitis. Total thirty six (36) patients were selected. Eighteen (18) were HBe(+ve) and another 18 were HBe(-ve). All had persistent or intermittent elevation of SGPT. Histological examination of liver biopsy specimen was done by Haematoxyn and Eosin (H & E) Stain. Histological activity index (Necroinflaminatory score) was calculated in both groups and compared. Among the patients with wild type of HBV the average HAI was 4.5. While patients with pre-core mutant chronic HBV infection the average HAI was 6.3. The difference between the two groups was not statistically significant. Average periportal necrosis among the patients with wild type was 1.88. While patients with precore mutant chronic HBV infection the average was 2.72. The difference between the two groups was statistically significant (P value<0.01). Average intralobular degeneration and local necrosis of the study group among the patients with wild type was 0.88, while patients with precore mutant chronic infection, the average were 7.7. The difference between the two groups was statistically significant (P value<0.5). Average portal inflammation among the patient with wild type was 1.66. While patients with precore mutant HBV infection the average was 2.11. The difference between the two groups was not statistically significant (P value>0.05). We also found that the progression of disease in terms of liver fibrosis rather than disease activity was more significant in PCM infection than in wild-type HBV infection.
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PMID:Histological Spectrum of Chronic Hepatitis in HBeAg Positive and HBeAg Negative Hepatitis B Virus Infection. 2662 3