Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0019158 (hepatitis)
 30,205 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To investigate the pathological differences between spontaneously developed and chemically induced liver cancers in LEC rats with hereditary hepatitis, eight-week-old LEC rats were fed 0.06% or 0.03% of 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) for 12 weeks, and then fed basal diet. Spontaneous liver cancers occurred in 60.4% of the LEC rats, while 3'-Me-DAB induced cancers in all LEC rats. The survival periods of chemically (0.06%) treated LEC rats were significantly shorter than non-treated rats. The chemically-induced cancers were strongly related to Edmondson classification. Metastasis and transplantability of the chemically-induced cancers were higher than spontaneous cancers. LEC rats are highly susceptible to the chemical carcinogen with the initiated status of hepatocarcinogenesis.
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PMID:Pathological comparison between spontaneously developed and chemically induced liver cancers in LEC rats with hereditary hepatitis. 2154 28

Metallothionein (MT) is known to be involved in various physiological roles and diseases. However, a standard method for MT measurement has not been established until recently. Therefore, we have developed an easy and specific enzyme-linked immunosorbent assays (ELISA) to determine MT-1 and MT-2. In order to evaluate the method we developed, MT-1/2 in liver, kidney and brain was determined in wild type (WT), MT-1/2 knockout (KO) and MT-3 KO mice, with and without Cd treatment. MT 1/2 in urine was determined in genetically disordered LEC rats (an animal model of Wilson disease). MT-1/2 concentrations in the liver, kidney and brain in MT-1/2 KO mice were significantly lower compared to those of WT and MT-3 KO mice. MT-1/2 concentrations in the livers of WT mice significantly increased with Cd administration, but not in MT-1/2 KO mice. Similar results were observed by immunohistochemical staining. To confirm the molecular weight (MW) of MT detected in organs by the ELISA, analysis with a Sephadex G-75 was performed. Two peaks of MT-1/2 (MW small and large) were detected in WT and MT-3 KO mice. The small MT peak was mostly depleted in MT 1/2-KO mice, while a large MT peak remained. A significant increase in MT-1/2 concentration was detected in the urine of LEC rats with age and especially at the hepatitis stage. In conclusion, MT-1/2 ELISA and immunohistochemical staining was highly correlated with MT-1/2 determination in experimental animal specimens and could be a robust analytical tool for physiological and toxicological studies.
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PMID:Metallothionein (MT) 1/2 expression in MT 1/2 and MT 3 knock-out mice and Long-Evans Cinnamon (LEC) rats. 2229 21


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