Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0019158 (hepatitis)
 30,205 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The presence of liver IgG Fc receptor sites was demonstrated in the liver tissue from 23 patients with liver diseases and 2 patients without liver lesions by the localization of soluble immune complexes of peroxidase-antiperoxidase (PAP). Cryostat sections of liver tissues were incubated with the complexes and the peroxidase activity was revealed histochemically. In the normal liver tissue, PAP were localized on the liver cell membrane, the Kupffer cells, and some of the sinusoidal walls. In acute hepatitis, a strongly positive reaction on swollen Kupffer cells was remarkable but positive reaction on the liver cell membrane was very weak. In chronic aggressive hepatitis, PAP were strongly positive on multiplied Kupffer cells and many PAP-positive infiltrated cells were observed at the area of piecemeal necrosis. However, the positive reaction on the liver cell membrane in patients with chronic aggressive hepatitis was generally fainter than in the normal cases without liver diseases. These results correlated well with the severity of liver cell necrosis. In chronic persistent hepatitis, the number of PAP-positive infiltrated cells in the portal area and positive Kupffer cells were fewer than in chronic aggressive hepatitis. Similar results were obtained with liver cirrhosis, and in particular, the liver cell membrane with regenerative nodules gave a positive reaction. A negative result was obtained by incubation with PAP-F(ab')2 alone. PAP reaction was significantly inhibited by pretreatment with aggregated human IgG, trypsin, and pronase but not with neuraminidase.
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PMID:Detection of liver IgG Fc receptors using soluble immune complexes of peroxidase-antiperoxidase. I. Detection in liver tissue from patients with liver diseases. 701 47

The presence of liver IgG Fc receptor sites was demonstrated in the liver tissue from C-57 black mice with D-Galactosamine (GalN)-induced hepatitis by the localization of soluble immune complexes of peroxidase-antiperoxidase (PAP). Cryostat sections of liver tissues were incubated with the complexes and the peroxidase activity was revealed histochemically. In the normal liver tissue, PAP were localized on the Kupffer cells, some of the sinusoidal walls, and the liver cell membrane mainly at the side of the sinusoid. Twenty-four hours after single administration of GalN (1,500 mg/kg body weight), positive PAP reaction was mainly observed on the membrane surface of infiltrating cells in the area of hepatocellular focal necrosis, and faintly observed on the degenerative hepatic cell membrane and the sinusoidal walls. After 48 hours, these features became more remarkable, especially at the sites with severe necrotic lesions. After 72 hours, the positive reaction on the regenerative hepatic cell membranes again became distinct. At the chronic stage 13 weeks after repeated administrations of GalN and Freund's complete adjuvant, a strong positive reaction was found on swollen and multiplied Kupffer cells, and a faint one found on the liver cell membrane, especially at the periphery of the lobules; moreover, many PAP-positive infiltrated cells were found in the area of piecemeal necrosis. The relationship between liver IgG Fc receptors and the severity of liver cell necrosis is discussed.
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PMID:Detection of liver IgG Fc receptors using soluble immune complexes of peroxidase-antiperoxidase. II. Detection in liver tissue from mice with D-galactosamine-induced hepatitis. 701 48