Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0019158 (hepatitis)
 30,205 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The angiotensin II (AT II) levels in plasma were measured 68 times by using radio-immunoassay in 30 patients with viral hepatitis B (HB) and in 35 healthy persons as the control group. The results showed that the AT II levels of patients with HB were much higher than those of the control group (P less than 0.001). They were 219.25 +/- 91.31 ng/L and 60.70 +/- 10.73 ng/L, respectively. These indicated that the renin-angiotensin-aldosterone system (RAAS) of the patients was in exciting state. The levels of 8 cases of severe chronic active hepatitis (SCAH) (AT II = 270.40 +/- 106.55 ng/L, 6 cases of subacute fulminant hepatitis (SFH) (AT II = 332.80 +/- 140.12 ng/L), and 4 cases of hepatocirrhosis (HC) (AT II = 218.50 +/- 97.64 pg/ml) were all higher than those of 8 cases of chronic active hepatitis (CAH) (100.50 +/- 83.81 ng/L) and those of 4 cases of acute icteric hepatitis (A1H) (123.33 +/- 64.97 ng/L). These findings showed that the levels of AT II were directly related with the severity of the illness. The AT II levels of 8 cases of HRS (270.50 +/- 66.31 ng/L) were higher that those without HRS (174.50 +/- 78.48 ng/L). After treatment with captopril (CPT), the renal function of the patients returned to normal and the patients got better, while the AT II levels decreased greatly. The results suggested that high AT II levels in plasma may be one of the causes aggressing the HRS. The CPT may inhibit the produce of AT II and there are some therapeutic effects for the HRS.
Hua Xi Yi Ke Da Xue Xue Bao 1991 Sep
PMID:[The relationship between hepatitis B and renin-angiotensin-aldosterone system]. 174 16

delta virus cDNA of China Sichuan strain, 1.2 kb in length, was synthesized by extraction of RNA from liver tissue in a patient with subacute severe hepatitis, followed by reverse transcription and polymerase chain reaction (RT-PCR). The result was confirmed by Southern blot hybridization using 32P and biotin labelled delta virus cDNA (HN88, cloned in USA) probes respectively.
Hua Xi Yi Ke Da Xue Xue Bao 1991 Mar
PMID:[Synthesis of delta virus cDNA of China Sichuan strain]. 177 23

Gurdon's Xenopus laevis oocyte translation system has been extensively employed to investigate the molecular biology of gamma-aminobutyric acid (GABA) receptors in the brains of embryonic chick, rat, etc. As GABA and its receptor may play a role in the pathogenesis of hepatic encephalopathy and a rabbit model of fulminant hepatitis has been established, we reconstituted the rabbit model of fulminant hepatitis has been established, we reconstituted the rabbit brain GABA binding protein (receptor) on Xenopus laevis oocytes to offer a base for further study of the pathological effects of GABA receptor at molecular level. In this report, total RNA was extracted from rabbit brain by guanidine hydrochloride method. The total RNA was then subjected to oligo-(dT)-cellulose affinity chromatography to isolate mRNA. All the RNA samples achieved a high purity with an average OD260/OD280 ratio of about 2.10. The clear bands of 18 S and 28S rRNA in electrophoresis implied that the RNA was not degraded and was therefore available for expression. After two days of incubation of the oocytes injected with mRNA, radioreceptor assay indicated that saturable and specifically displaceable GABA binding sites were implanted onto the oocyte membrane. This result led us to the conclusion that rabbit brain GABA binding protein (receptor) can be reconstituted by injecting exogenous mRNA inclusive of those encoding the receptor into Xenopus oocytes. However, the functional activity of the reconstituted receptor as a GABA gated chloride ion channel needs to be further characterized by patch clamping.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Sep
PMID:[Reconstitution of rabbit brain GABA binding protein (receptor) on Xenopus oocytes]. 196 92

To study the expression of Pre-S1 and Pre-S2 and gene-encoded proteins of the hepatitic B virus, we investigated serum from 11 patients with chronic type B hepatitis in various phases, Pre-S1 and Pre-S2 proteins were detected by the monoclonal antibody-based enzyme method, the markers of active viral replication were determined with the presence of HBV-DNA and HBeAg. It was found that Pre-S1 was present in the serum of 3 patients with viral replication as well as of 6 out of 8 cases with no evidence of viral replication, Pre-S2 in the serum of 2 out of 3 patients with active viral replication as well as 4 out of 8 cases with no evidence of viral replication. The similar results also appeared in patient's serum in various phases. The data suggest that there is no correlation between the presence of the Pre-S1 or Pre-S2 proteins and active viral replication during the course of chronic type B hepatitis.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Sep
PMID:[Expression of Pre-S1 and Pre-S2 in serum of patients with chronic type B hepatitis]. 209 34

The epidemiological features of various types of acute viral hepatitis in 5 regions of Sichuan Province (211,639) population) form Feb.16, 1987 to Feb 15, 1988 were studied. One-year surveillance was carried out and the results showed an incidence rate of 167.74 per 100,000 for acute viral hepatitis. The proportional distribution of HA,HB,non-A,non-B Hepatitis (HNANB), Hepatitis with EBV (HEB), Hepatitis with CMV (HCM) and Mixed infection in all the patients being 24.51%, 38.31%, 24.51%, 3.38%, 3.38% and 5.92%, respectively. There was a proportion of 14.93% for superinfection. The incidence differences in these regions ranged from 110.30 per 100,000 to 299.95 per 100,000. The incidence of hepatitis peaked in spring, and the sex ratio (male:female) for acute viral hepatitis was 1.75:1 (P less than 0.001). There was a peak of attack rate for HA in 0-19 age group. The incidence of HB in 20-39 age group was obviously higher than that in other age groups. For HNANB, a relatively high attack rate was noticed in 5-19 age group, but no significant age-differences were noted for HEB,HCM and Mixed. Of 355 patients with acute viral hepatitis, 43.94% had a history of contact, and 36.62% a history of injection.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Sep
PMID:[A population-based study on types of viral hepatitis]. 212 48

We detected the presence and distribution of HBcAg in the liver by immunohistochemistry (ABC method) and the presence of HBV-DNA in serum (spot hybridization) and anti-HBe in serum (ELISA) from 59 cases of hepatitis B hospitalized in our hospital, including 47 cases of CAH, 5 cases of CPH, and 7 cases of subacute fulminant hepatitis. 1. HBcAg in the liver was detected in 25 out of 47 cases (53%) of CAH, in 2 out of 5 cases of CPH and in 4 out of 7 cases of subacute fulminant hepatitis. The total percentage was 53% (31/59). 2. There was no positive correlation between HBV replication activity and liver disease activity (P greater than 0.05). Our results did not support the hypothesis that suggests a direct cytopathic effect of HBV. Oppositely, the fact was that the presence, the amount and the patterns of HBcAg in the liver, and the presence of HBV-DNA in serum were predominant in mild CAH compared with those in severe CAH, predominant in CAH without cirrhosis compared with those in CAH with cirrhosis. There was a tendency of inverse correlation between HBV replication activity and liver disease activity. The results above were in line with the concept that HBcAg expressed on the surface of infected hepatocytes may be relevant target for T lymphocyte cytotoxicity. The results have suggested that an immune response to HBV is present, leading to the destruction of most infected cells. 3. There was a positive correlation between HBV-DNA in serum and HBcAg in the liver (P less than 0.005), indicating that HBV-DNA in serum can represent HBV replication.(ABSTRACT TRUNCATED AT 250 WORDS)
Hua Xi Yi Ke Da Xue Xue Bao 1989 Jun
PMID:[Relationship between HBcAg in the liver and mechanisms of chronic type B hepatitis HBVM in serum]. 259 35

Molecular weight of human hepatocellular carcinoma (HCC)-associated antigen-HL2 antigen was detected to be about 50,000 dalton by SDS-PAGE and Western blot. One hundred and twenty-seven paraffin sections of cancers and pathological liver tissue were tests by ABC immunohistochemical staining. MAb HL2 reacted with 62.7% HCC and also with 8 of the 10 stomach carcinomas but only with a few other digestive system carcinomas (pancreas carcinoma, rectum carcinoma and duodenum carcinoma) and hepatitis. MAb HL2 was negative in hepatocirrhosis and other tumors (vesica carcinoma, skin carcinoma, breast carcinoma, neurogliocytoma and carcinoma of the lung). The results suggest that MAb HL2 has values in diagnosis of HCC and differential diagnosis between HCC and other tumours. HL2 antigen might be a new tumor-associated antigen (TAA). Further study of HL2 antigen will significantly show the actions of TAA in the occurrence and development of tumor.
Hua Xi Yi Ke Da Xue Xue Bao 1994 Mar
PMID:[Immunohistochemical observation of anti-human hepatocellular carcinoma monoclonal antibody HL2 in cancers]. 807 Jul 68

We determined the Guanine Deaminase (GD) activity of 200 patients with different diseases. It was found that GD activity of hepatic patients is higher than that of health adults, while the GD activity of other patients is in the normal range. There is a linear correlation between GD activity and ALT in patients with chronic hepatitis, billiary obstruction, and between GD activity and total bilirubin in patients with chronic active hepatitis, biliary obstruction and liver cirrhosis. Moreover, the GD activity of patients positive for anti-HCV is significantly increased. So GD activity in serum is a specific and sensitive index to estimating hepatic functions and can be used in the diagnosis of acute and chronic hepatitis, cirrhosis of liver, and C virus hepatitis.
Hua Xi Yi Ke Da Xue Xue Bao 1996 Jun
PMID:[The value of determining guanine deaminase in diagnosis of hepatic diseases]. 938 40

The levels of serum acidic isoferritin (SAIF) in 48 patients with hepatocellular carcinoma (HCC), 30 patients with hepatitis, 28 patients with liver cirrihosis, and 33 healthy subjects were measured by using enzyme-linked immunosorbent assay (ELISA--double-determinant). The result revealed that the SAIF values of HCC, HP and LC did not show normal distribution. The median values were 440 micrograms/L, 21 micrograms/L, 120 micrograms/L and, in normal subjects, 66 micrograms/L respectively. There were statistically significant differences between HCC and the control groups. The sensitivity of SAIF to HCC was 85.46%, with the cut-off point being 250 micrograms/L. SAIF was not correlated to AFP in HCC cases. The values of SAIF had no relationship with the volume of the tumors and the clinical stages. SAIF may be an available and useful serum marker and be beneficial to the diagnosis of early-staged HCC.
Hua Xi Yi Ke Da Xue Xue Bao 1997 Dec
PMID:[Observation of the serum acidic isoferritin levels in patients with hepatocellular carcinoma]. 1068 60

In order to explore the specificity of serum bile acid (SBA) chromatograph in the diagnoses of different kinds of hepatosis, we investigated by means of gas chromatography the changes of serum bile acids in workers who exposed to hexogen or chloroethylene and in patients who suffered from hepatosis such as acute jaundice hepatitis, chronic active hepatitis, cirrhosis and liver cancer. The results revealed different disturbances of SBA occurring in the liver injuries induced by the two kinds of hepatotoxicant. Serum lithocholic acid (LCA), deoxycholic acid (DCA) and chenodeoxycholic acid (CDCA) in workers exposed to hexogen and wre significantly different from those of the control group respectively (P < 0.01, P < 0.01, P < 0.05); on the other hand, only serum LCA and DCA went up in workers exposed to chloroethylene (P < 0.0005, P < 0.001). The main changes both concentrated on the secondary bile acids. In acute jaundice hepatitis, chronic active hepatitis, cirrhosis and liver cancer, serum LCA, DCA, CDCA and cholic acids (CA) all went up in different degrees compared with the control group respectively (P < 0.005, P < 0.025, P < 0.005, P < 0.005). But no difference was noted among the 4 kinds of bile acids (P > 0.5), except that between CA and CDCA. These provided the evidence of the diagnosis and identification of clinical hepato-biliary diseases and occupational liver injures.
Hua Xi Yi Ke Da Xue Xue Bao 1997 Mar
PMID:[Application of serum bile acid chromatography to the diagnoses of liver diseases]. 1068 67


1 2 Next >>