Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0018801 (
heart failure
)
72,216
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Real-time quantitative reverse transcriptase-PCR (qRT-PCR) is a feasible tool for determining gene expression profiles, but the accuracy and reliability of the results depends on the stable expression of selected housekeeping genes in different samples. By far, researches on stable housekeeping genes in human
heart failure
samples are rare. Moreover the effect of
heart failure
on the expression of housekeeping genes in right and left ventricles is yet to be studied. Therefore we aim to provide stable housekeeping genes for both ventricles in
heart failure
and normal heart samples. In this study, we selected seven commonly used housekeeping genes as candidates. By using the qRT-PCR, the expression levels of ACTB, RAB7A, GAPDH,
REEP5
, RPL5, PSMB4 and VCP in eight
heart failure
and four normal heart samples were assessed. The stability of candidate housekeeping genes was evaluated by geNorm and Normfinder softwares. GAPDH showed the least variation in all heart samples. Results also indicated the difference of gene expression existed in
heart failure
left and right ventricles. GAPDH had the highest expression stability in both
heart failure
and normal heart samples. We also propose using different sets of housekeeping genes for left and right ventricles respectively. The combination of RPL5, GAPDH and PSMB4 is suitable for the right ventricle and the combination of GAPDH,
REEP5
and RAB7A is suitable for the left ventricle.
...
PMID:Selection of reference genes for gene expression studies in heart failure for left and right ventricles. 2839 Sep 90
The sarco-endoplasmic reticulum (SR/ER) is the largest membrane-bound organelle in eukaryotic cells and plays important roles in essential cellular processes, and in development and progression of many cardiac diseases. However, many aspects of its structural organization remain largely unknown, particularly in cells with a highly differentiated SR/ER network. In a recently published study led by Lee
et al.
(Nat Commun 11(1):965), we reported a cardiac enriched SR/ER membrane protein
REEP5
that is centrally involved in regulating SR/ER organization and cellular stress responses in cardiac myocytes.
In vitro
REEP5
depletion in mouse cardiac myocytes resulted in SR/ER membrane destabilization and luminal vacuolization along with decreased myocyte contractility and disrupted Ca
2+
cycling. Further,
in vivo
CRISPR/Cas9-mediated
REEP5
loss-of-function zebrafish mutants showed sensitized cardiac dysfunction to
heart failure
induction upon short-term verapamil treatment. Additionally,
in vivo
adeno-associated viral (AAV9)-induced
REEP5
depletion in the mouse demonstrated cardiac dysfunction with dilated cardiac chambers, increased cardiac fibrosis, and reduced ejection fraction. These results demonstrate the critical role of
REEP5
in SR/ER organization and function.
...
PMID:Towards understanding the role of Receptor Expression Enhancing Protein 5 (REEP5) in cardiac muscle and beyond. 3207 61
