Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0018133 (graft-versus-host disease)
 18,032 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In vitro cultures of mouse bone marrow cells, maintained for periods up to 7 wk, were shown to contain cells able to repopulate irradiated hosts with T and B lymphocytes. The lymphocytes were fully functional and there did not appear to be any gross restriction of their receptor repertoire. The cultured cells reconstituted irradiated semiallogenic hosts without evidence of graft-versus-host disease, suggesting that culture of donor marrow might be a useful preliminary to transplantation when tissue matching is incomplete.
 ...
PMID:In vitro studies on lymphocyte differentiation. I. Long term in vitro culture of cells giving rise to functional lymphocytes in irradiated mice. 2 38

Graft-versus-host disease, a complication of allogeneic bone-marrow transplantation, involves primarily the skin, liver and intestines, but may also be associated with pneumonia. To determine the relation of graft-versus-host disease with pneumonia, we evaluated the autopsies of 59 allogeneic and two autologous recipients and 74 control patients with various pulmonary diseases, who had not received a bone-marrow transplant. Lymphocytic bronchitis, characterized by lymphocyte-associated necrosis of the bronchial mucosa and often the submucosal glands, was present in 12 of 20 patients with Grade 2 or greater graft-versus-host disease but in only three of 39 with Grade 0 to 1 disease (P less than 0.0005). Onset of respiratory disease correlated with the time of onset of graft-versus-host disease. Patients with lymphocytic bronchitis had a higher incidence of bronchopneumonia and acute bronchitis of the lower respiratory tract. Lymphocytic bronchitis did not occur in the controls and appears to be a component of graft-versus-host disease that leads to bronchopneumonia, probably through destruction of the mucociliary apparatus.
 ...
PMID:Lymphocytic bronchitis associated with graft-versus-host disease in recipients of bone-marrow transplants. 3 44

Seventeen patients with aplastic anemia or acute leukemia received transplants from donors who had major ABO incompatibilities. Antibody titers were decreased by plasma and whole blood exchanges prior to marrow infusion. All 17 patients were successfully engrafted, and there was one possible rejection in the patient with the highest pretransplant anti-A IgG titer. Nine of 17 patients are currently alive. A review was carried out of transplants performed in Seattle between HLA-matched siblings with aplastic anemia and leukemia. Two hundred forty-six evaluable patients with ABO-compatible donors were compared with 46 with minor ABO-incompatible donors. There was no effect of minor ABO incompatibility on graft rejection, incidence and severity of graft-versus-host disease, or survival.
 ...
PMID:ABO-incompatible marrow transplants. 3 Jan 94

Methotrexate (MTX) followed by citrovorum factor (CVF) rescue was evaluated for its effectiveness in reducing graft-versus-host disease (GVHD) in lethally irradiated dogs transplanted with bone marrow from unrelated histoincompatible donors. Animals were given no immunosuppressive therapy (group A) or a combined regimen of MTX and CVF (group AMC). These two groups were compared with a group of animals transplanted earlier given MTX alone (group AM). Ainmals in the AMC group lived significantly longer than the A group (p less than 0.05). Engraftment rate, hematopoietic recovery and incidence of GVHD were similar in all three groups. Incidence of early deaths was significant in the AM group (p less than 0.05). It is concluded that MTX combined with CVF increases survival and is an effective posttransplantation immunosuppressive regimen with minimal toxicity.
 ...
PMID:Methotrexate and citrovorum factor after histoincompatible allogeneic bone marrow transplants in dogs. 3 Feb 47

Forty-one patients, suffering from severe aplastic anaemia were treated either with ALG alone (27 patients) or ALG followed by infusion of allogeneic bone marrow (14 patients). Eighteen patients (67 per cent) are presently alive after ALG alone at over 100 to over 550 days. Fourteen (52 per cent) showed sustained improvement of haematopoiesis, two are alive without change, one recovered autologous haematopoiesis after cyclophosphamide conditioning and transfusion of HLA identical marrow and one is lost to follow-up. Eight patients (57 per cent) are currently alive after ALG and transfusion of haplotype identical marrow with self-sustaining autologous haematopoiesis at over 200 days to over four and a half years. No lethal complications occurred and none of the bone marrow infusions led to permanent engraftment or graft-versus-host disease. The mechanism of action is not known, but our results support the hypothesis that unspecified autoimmune reactions block the normal outgrowth of haematopoietic precursor cells in a substantial number of patients with aplastic anaemia. This therapeutic approach seems to offer good chances of survival, especially for those patients who do not have an HLA identical sibling. Its value should be further investigated.
 ...
PMID:Treatment of aplastic anaemia by antilymphocyte globulin with or without marrow infusion. 3 Dec 56

12- to 36-hours-old newborn CBA mice were intraperitoneally injected each with 10 X 10(6) allogeneic spleen cells of adult C57Bl mice. Control mice received syngeneic spleen cells of adult CBA mice either in the same way or remained untreated. The animals were killed 1, 3, 7, 14 or 21 days after the spleen cell injection. The pancreas was studied histologically and electron microscopically by common methods. Together with an interstitial lymphohistiocytic infiltration of the pancreas different acinar cell alterations were observed in the mice treated with allogeneic spleen cells: 1. Acute lethal pancreatic cell damages on the day after the intraperitoneal injection of allogeneic spleen cells. 2. Membrane-lined inclusion vacuoles between the 7th and 21st experimental day. 3. Atrophy of pancreatic acinar cells in the terminal stage of a severe graft-versus-host disease. The pathogenesis of the observed pancreatic changes seems to be due to nonimmunological and immunological processes. The membrane-lined inclusion vacuoles of the acinar cells could be the consequence of a cell-mediated immune process in the graft-versus-host reaction.
 ...
PMID:Ultrastructural findings in the mouse exocrine pancreas during graft-versus host reaction. 3 Dec 94

Transplantation of allogeneic bone marrow both from normal and thymus-deprived donors (thymectomized lethally irradiated and restored with autologous bone marrow) was followed 6--7 days later by the appearance of large numbers of GVH cells in bone marrow and peripheral blood which were one of the characteristic features of acute GVHR. GVH cells isolated from peripheral blood 7 days after transplantation showed a high labelling index (about 30%) after 40 min incubation with 3H-thymidine. The prolongation of incubation failed to increase the proportion of labelled cells due to the low viability of GVH cells. The results obtained with T and B cell tests suggested that GVH cells had the properties of T lymphocytes: about 60% of the cells could form rosettes with non-sensitized sheep red blood cells; there were virtually neither Ig-bearing cells nor cells with receptors for C3 and Fc portion of Ig.
 ...
PMID:Transplantation of allogeneic bone marrow from thymus-deprived monkeys and certain characteristics of "graft-versus-host" cells. 3 79

Mortality in nude mice fostered by allogeneic mothers was studied. The data failed to support the hypothesis that mortality was due to graft versus host disease resulting from the passage of immunocomponent cells to the young in the mothers' milk. It was apparent that there were significant differences in the ability of mothers of different strains to rear nude mice.
 ...
PMID:The effect of fostering nude mice with allogeneic mothers on subsequent mortality. 3 14

A patient with aplastic anemia who was found to be homozygous for an HLA-D determinant shared by her unrelated parents achieved sustained engraftment and full restoration of hematopoietic and lymphoid function following a transplant from an HLA-A and -B nonidentical, ABO incompatible sibling who was heterozygous for the shared HLA-D specificity. Transplantation was complicated by transient graft-versus-host disease of moderate severity, which resolved completely following treatment with antithymocyte globulin and prednisone. The case indicates that patients found to be HLA-D-homozygous may be successfully transplanted from HLA-D-heterozygous sibling donors despite HLA-A and HLA-B incompatibilities, and thus further demonstrates the importance of the HLA-D region as a marker of donor-host histocompatibility.
 ...
PMID:Successful transplantation of marrow from an HLA-A, -B, -D mismatched heterozygous sibling donor into an HLA-D-homozygous patient with aplastic anemia. 3 52

Cyclophosphamide (CY) was activated in vitro with washed rat liver microsomes and cofactors. Pretreatment of mouse spleen cells in vitro with the activated drug abolished their capacity to give a primary antibody response to SRBC and levan on transfer to irradiated syngeneic recipients. However, responsiveness returned if challenge was delayed for 7 or more days after transfer. Part of this was shown to be of donor origin by an allotype marker. The treatment of normal spleen cells with activated CY in vitro also prevented B cells from regenerating their immunoglobulin receptors after capping with anti-immunoglobulin serum. The induction of suppression required contact between lymphocytes and activated CY for at least 30 min at 37 degrees and did not appear following incubation for 1 h at 0 degrees. Since the antibody response of drug-treated spleen cells to SRBC could not be restored with purified normal B or T cells, it is probable that B and T lymphocytes are both susceptible to suppression by activated CY in vitro. Similar pretreatment abrogated the graft-versus-host (GVH) reactivity of spleen cells as measured by survival and in a popliteal lymph node assay. B cell chimerism in F1 recipients of drug-treated parental spleen cells was demonstrated by the presence of congenic allotype markers. This suggests a possible approach for the attenuation of GVH disease which is associated with bone marrow transplantation in man.
 ...
PMID:The capacity of microsomally-activated cyclophosphamide to induce immunosuppression in vitro. 3 18


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>