Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0018133 (graft-versus-host disease)
 18,032 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Growth of transplantable KMT-17 tumour in syngeneic WKA/MK rats was inhibited by i.v. preimmunization with whole blood from normal rats of allogeneic strains. The inhibitory effect was also observed in rats immunized with allogeneic white blood cells alone. The strength of the inhibitory effect mainly depended on the the strain of donor rat used; blood from Donryu and Kyoto strain rats produced the strongest inhibition, blood from Tokyo and Fischer strain rats produced moderate inhibition to the syngeneic tumour growth. Blood from ACI/N strain rats did not produce the inhibition. The mechanism of blood transfusion in inhibiting tumour growth is not yet clear. However, it seems that GVH reaction does not play an important role in the mechanism of the inhibition effect, because the effect was obtained by immunization with mitomycin C-treated allogeneic white blood cells and also by the immunization effect may be due to nonspecific active immunization. Significance of blood transfusion with special reference to clinical immunogtherapy of cancer is discussed.
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PMID:Anti-tumour immunity by normal allogeneic blood transfusion in rat. 1 11

The effect of the tritiated thymidine (3H-TdR) suicide technique on the ability of donor cells to induce fatal graft-versus-host disease (GVHD) was studied. C57BL/6 (H-2b) spleen cells were stimulated in vitro with irradiated BALB/c (H-2d) Moloney lymphoma cells in mixed culture and 3H-TdR of high-specific activity added to eliminate proliferating cells. The ability of such cells to induce fatal GVHD was assayed by injecting them i.v. into adult BALB/c mice immunosuppressed with cyclophosphamide (180 mg/kg). These cells induced fatal GVHD in fewer mice (52 per cent) than did C57BL/6 cells cultures with BALB/C lymphoma cells but without 3H-TdR (87%) and C57BL/L cells cultured with irradiated C57BL/6 cells with (95 per cent) or without 3H-TdR (86 per cent). Thus, the 3H-TdR suicide technique greatly diminished the ability of cells to induce lethal GVHD.
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PMID:Reduction of fatal graft-versus-host disease by 3H-thymidine suicide of donor cells cultured with host cells. 1 80

Improvements in the results of bone marrow transplantation for the treatment of SCID may be expected by employing purified stem-cell concentrates for patients who do not have a compatible sibling available. Refinements in the purification technique and its monitoring are required, however. For the same category of patients it seems worthwhile to continue attempts at restoration with liver cells from fetuses less than 12 weeks of age. In addition, full protection against infections should be provided for patients expected to develop GVHD, and, therefore, such patients should only be treated in centers where reverse isolation and bacteriologic decontamination can be performed. In view of the rarity of the disease, transplanters should agree on a limited number of graft protocols. For the treatment of bone marrow aplasia, attempts to identify the factors that can serve to predict the occurrence of GVHD in compatible host-donor sibling pairs should be continued. Only when the patients who will develop GVHD can be recognized in advance will it be feasible to fully exploit available GVHD reductive measures. In particular the role of the intestinal microflora should be investigated in this respect. Experimental evidence is presented, suggesting an aggravating influence of microflora on GVHD lesions, which are primarily induced by histocompatibility reactions. For such studies with incompatible siblings, the dog is the best available animal model. For the selective isolation of hemopoietic stem cells for transplantation purposes (as one means of reducing GVHD), methods for rapid identification of stem cells and immune competent cells, respectively, have to be developed. In leukemia, more research is necessary on the factors that play a role in the late complications of bone marrow transplantation. The toxicity of aggressive regimens employed in the eradication of the leukemia should be further analyzed. The collection of autologous normal hemopoietic stem cells from leukemic patients as introduced by Dicke et al. warrants further exploration to see whether these cells may replace the allogeneic transplantation procedure, thus avoiding all the complications generally encountered in GVHD. For all three diseases, it is extremely important to develop a method for the selection of compatible donors among unrelated individuals, because this will at least double the number of candidates for therapeutic bone marrow transplantation. Current progress in histocompatibility typing in the rhesus monkey and the dog makes these species excellent models for such investigations.
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PMID:Bone marrow transplantation. 1 88

The experimental data show that absorption of ATG with liver-kidney homogenate and CLL and LCL cells stepwise removed the hemopoietic toxicity, whereas the specific activity against T lymphocytes remained. Although the mode of action of absorbed ATG could not be tested in the first clinical case, the successful experiments in rodents together with the fact that the incubation treatment was tolerated by the patient may provide a new way of preventing fatal GVH reactions in man.
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PMID:Specific absorbed antithymocyte globulin for incubation treatment in human marrow transplantation. 1 92

By administering anti-mouse ATS five times to random-bred Swiss albino mice weighing 16-18 and 10-11 g respectively, the authors have tried to produce immunological depression of such a degree which would allow living human leukocytes to cause GVH reaction. On the basis of the results it may be stated that with the serum dosage and manner of treatment applied, the human graft was not able to act against the recipient organism and to produce differences in body weight.
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PMID:Experiments for the production of graft-versus-host reaction in adult and infantile mice by means of human immunocompetent cells. 1 47

It was not possible to produce the wasting disease in newborn mice by administering human leukocytes in Hanks' solution, or suspended in m-ATS or h-ATS. The attempt of the authors to produce immunosuppression by the administration of m-ATS started at newborn age-as a result of which the human immunocompetent cells would have produced serious GVH reaction-resulted only in a trend of GVH. The authors used only body weight measuring for detecting the GVH reaction. More sensitive parameters will be employed in their experiments in progress.
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PMID:Exeriments for the production of graft-versus-host reaction in newborn mice by means of human immunocompetent cells. 1 48

F1 hybrid mice (C57BL X C3H) suffering from graft-versus-host disease, were treated with dianhydrodulcit (DAD) a lymphotrop cytostatic agent. 15 mg/kg of the compound was injected intraperitoneally, 8 days following the administration of the parental spleen cell suspension. The interaction between the two interventions is discussed on the basis of the rate and time course of deaths observed is the individual groups, as well as on the state of the lymphoid organs of the succumbed and sacrificed animals.
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PMID:Study on the interaction of lymphotropic cytostatic agent treatment and graft-versus-host (GVH) reaction in mice. 1 49

Baboons were given 1200 R total body irradiation from two opposing 60Co sources. Three animals were given supportive therapy only and died, as expected, within 8 days of irradiation with profound marrow hypoplasia. Five baboons were cross-circulated with unirradiated partners and died within 14 days with evidence suggestive of graft-versus-host disease. Their marrows were repopulated with hemopoietic precursor cells, and three of the five had rises in peripheral white blood cell counts to more than 1500/cu mm before death. These results are compatible with the presence of hemopoietic stem cells in the peripheral blood of a nonhuman primate, the baboon.
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PMID:Demonstration of hemopoietic stem cells in the peripheral blood of baboons by cross circulation. 1 39

Profound lymphocyte depletion occurs in thymus, lymph nodes and spleens of normal and thymectomized mice early after a single high sublethal dose of cyclophosphamide. Among individual lymphocytes characterized by the different types of nucleolus, the "non-activable" lymphocytes with micronucleoli are affected most markedly by CY, whereas the proportion of the "active" lymphocytes is increased (up to 70% in the spleens). During the period of regeneration lymphocytes with micronucleoli increase rapidly, the increment thereof is very high on day 14 in the spleens of all mice, and still higher in the blood and lymph nodes of thymectomized animals. During the period of lymphocytopenia, in spite of the increased proportion of the "active" lymphocytes, the ability of residual cells of elicit GVH reactions and to incorporate 14C-uridine decreases. In contrast to changes in the early period, the recovery of the "active" lymphocytes and GVH reactivity, which is still incomplete on day 14, depends on the presence of an intact thymus.
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PMID:Nucleolar and functional characterization of lymphocytes following cyclophosphamide treatment. 1 71

Pretransplant and posttransplant data for 69 patients with severe combined immunodeficiency disease are presented. Both B and T lymphocyte functions were absent in approximately 80% of the children and markedly depressed in the remainder. Transplantation of marrow from HLA genotypically identical donors provided the highest six-month survival rate (63%); six-month survival rates for patients who received fetal tissue transplants (43%) or marrow from mixed leukocyte culture (MLC) negative donors (38%) were significantly higher (P less than .05) than for patients treated with marrow from MLC positive donors (5%). Additional factors appeared to influence survival and the severity of graft-vs-host (GVH) disease. Patients more than 6 months of age had more intense GVH disease than younger patients. Survival rates were lower and GVH disease more intense when boys received transplants from girl donors than the reverse.
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PMID:Severe combined immunodeficiency disease. Characterization of the disease and results of transplantation. 1 18


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