Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0017638 (
glioma
)
30,880
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Background:
Glioma
is the most common brain tumor with poor prognosis all over the world. Anesthetics have been demonstrated to have important impacts on cell migration and invasion in different cancers. However, the underlying mechanism that allows anesthetics-mediated progression of
glioma
cells remains elusive.
Methods:
Sevoflurane (Sev), a class of common anesthetics, was used to expose to U87-MG and U251 cells. The expressions of microRNA-146b-5p (miR-146b-5p) and
matrix metallopeptidase 16
(
MMP16
)were measured by quantitative real-time polymerase chain reaction or western blot. Transfection was performed in
glioma
cells with miR-146b-5p inhibitor, inhibitor negative control,
MMP16
overexpression vector, empty vector, small interfering RNA against
MMP16
or scramble. Cell migration and invasion were analyzed by the trans-well assay. The interaction between miR-146b-5p and
MMP16
was explored by luciferase activity and RNA immunoprecipitation assays.
Results:
Sev treatment inhibited migration and invasion of
glioma
cells. The expression of miR-146b-5p was enhanced and
MMP16
protein was decreased in
glioma
cells after exposure of Sev. Knockdown of miR-146b-5p or overexpression of
MMP16
reversed Sev-induced inhibition of migration and invasion of
glioma
cells. Moreover,
MMP16
was indicated as a target of miR-146b-5p and its silencing attenuated the regulatory role of miR-146b-5p abrogationin Sev-treated
glioma
cells.
Conclusion:
Sev impeded cell migration and invasion through regulating miR-146b-5p and
MMP16
in
glioma
, indicating a novel theories foundation for the application of anesthetics like Sev in
glioma
.
...
PMID:Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16. 3138 37
