Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0017636 (
glioblastoma
)
18,345
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This study probes the function and mechanism of
lymphocyte-specific protein 1
(
LSP1
) in
glioblastoma
pathogenesis. According to the data acquired from TCGA, Oncomine and GEO databases, the expression and prognostic value of
LSP1
and miR-920 in
glioblastoma
patients were analyzed. The expression levels of
LSP1
in U251 and A172 cell lines were analyzed by qRT-PCR and western blotting. CCK8, colony formation and transwell assays were utilized to test
glioblastoma
cell malignant abilities. Furthermore, the associations between
LSP1
and miR-920 were indentified by bioinformatics analysis and rescue assays. Moreover, the protein expression levels of p-JAK2, JAK2, p-STAT5 and STAT5, as the hallmark of JAK/STAT5 signaling, were detected by western blotting. The observations showed that
LSP1
was highly augmented in
glioblastoma
samples. Additionally, up-regulation of
LSP1
was associated with a unfavorable prognosis in
glioblastoma
patients. Biological experiments revealed that depletion of
LSP1
significantly suppressed the proliferation, invasion and migration of U251 and A172 cells. MiR-920, as an upstream regulator of
LSP1
, negatively modulated
LSP1
expression and promoted U251 cells malignant behaviors after miR-920 inhibitor treatment. However, together knockdown
LSP1
and miR-920 inhibited these effects. Moreover, the expression levels of p-JAK2 and p-STAT5 were increased or decreased in U251 cells after transfection of miR-920 inhibitor or si-LPS1. Taken together, miR-920 might blocked the malignant development of
glioblastoma
cells, which is possibly realized by targeting
LSP1
and modulation of JAK/STAT5 pathway. These findings implied that miR-920/
LSP1
was a potential therapeutic target for
glioblastoma
treatment.
...
PMID:MiR-920 and LSP1 co-regulate the growth and migration of glioblastoma cells by modulation of JAK2/STAT5 pathway. 3277 Feb 94
