Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0017636 (
glioblastoma
)
18,345
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Through a strategy of direct cloning of TP53-binding DNA sequences from human genome DNA, we have identified a novel
TP53-target gene
, termed
TP53TG5
(
TP53-target gene
5). This gene, localized to chromosome band 20q13.1 by fluorescence in situ hybridization, encodes a 290-amino-acid peptide with no significant homology with any known proteins in the public database. A colony-formation assay using human
glioblastoma
cell line T98G, which lacks wild-type TP53 and expresses no endogenous
TP53TG5
, revealed a growth-suppressive effect of the
TP53TG5
gene product. Furthermore, immunohistochemical studies, following transfection of T98G with plasmid designed to express green fluorescent protein-fused
TP53TG5
, revealed cell cycle-dependent intracellular localization of this protein. Our results suggest that functional studies of
TP53TG5
may provide new insights into the complex physiological activities of TP53.
...
PMID:Isolation and characterization of a novel TP53-inducible gene, TP53TG5, which suppresses growth and shows cell cycle-dependent transition of expression. 1071 63
