Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0017536 (giardiasis)
 1,714 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Giardia lamblia antigens which react with sera from children with G. lamblia infection were investigated by sodium-dodecyl polyacrylamide gel electrophoresis and immunoblotting. Serum IgG, IgM and IgA response to the antigens were immunochemically characterized. Serum antibodies from all giardiasis patients, but none of the controls, was found to react with a 57-kilodalton antigen. The 57 kDa antigen elicited IgG and IgA but not IgM antibodies. The protein nature of the 57 kDa antigen was demonstrated by loss of antibody recognition after trypsin treatment of G. lamblia trophozoites. Subcellular fractionation of G. lamblia trophozoites followed by SDS-PAGE and immunoblotting showed that the 57 kDa antigen was probably not a component of the cytoskeleton.
 ...
PMID:Serum antibody response in children with Giardia lamblia infection and identification of an immunodominant 57-kilodalton antigen. 185 78

Entamoeba histolytica--specific serum IgG, IgA, IgM and IgE were assayed in cases of amoebiasis in an endemic area. Patient groups consisted of amoebic liver abscess (n = 18), pre abscess hepatic amoebiasis (n = 22) and amoebic colitis (n = 30). Control subjects comprised 26 asymptomatic cyst passers, 13 giardiasis cases, 20 typhoid patients and 24 non amoebic individuals. Serum IgG was assayed by ELISA: using a monoclonal anti IgG beta-galactosidase (IgG beta-gal) conjugate, a polyclonal avidin biotin horse radish peroxidase (AB-HRP) and a polyclonal anti IgG horse radish peroxidase (IgG HRP) conjugate. IgA and IgM were assayed by the beta-gal ELISA and IgE by AB-HRP. Diagnostically significant IgG and IgA while lower IgM and IgE levels were seen in extra intestinal cases. About 40% of suspected pre abscess hepatic amoebiasis cases were confirmed by antibody estimation. All isotype levels in most dysentery cases were in the range of the controls. Inter assay coefficient of variation and assay specificity/sensitivity are also discussed.
 ...
PMID:Detection of IgG, IgA, IgM and IgE in antibodies in invasive amoebiasis in endemic areas. 213 1

Local humoral response of the intestinal mucosa was determined with secretory IgA levels and secretory component activity in enterocytes and duodenal content of 15 children infected with G. intestinalis. The obtained results were compared to those in 5 children with coeliac disease and 12 children with diarrhoea without lambliasis. Secretory IgA was increased in about 50% of children with lambliasis (in the remaining groups in 25% of children) to the values higher than that in the comparative groups. Secretory component activity was relatively high in the intestinal epithelium. Secretory component activity in the duodenal content was high in about 40% of children independently of the examined group. No correlation between the said variables was noted except positive correlation of secretory IgA levels and secretory component activity in the bile.
 ...
PMID:[Humoral response of the immunologic secretory system of the small intestine in children infected with Giardia intestinalis]. 221 59

Whether use of oral contraceptives depresses the immune system in Indian women, and whether they affect the prevalence of giardiasis in users, was explored by testing immune function and stools for Giardia lamblia in 14 pill users and 10 controls. The subjects were aged 18-40 and had taken a low dose oral contraceptive for at least 6 months. The immune functions examined were quantitative serum IgG, IgA and IgM levels by radial immunodiffusion, cell-mediated response to phytohemagglutinin, and peripheral T-cell counts. 1 pill user had signs of giardia infection. Immunoglobin levels in the other were not significantly different from controls, except for IgM, which was 415.34 mg/dl in pill users, vs. 251.69 in controls. T-cells were normal in 1 pill user tested. The mean blastogenic index, or lymphocyte transformation index, was significantly depressed at 6.6 in pill users as well as the woman with giardia, compared to 17.6 in controls. The reported incidence of giardiasis in an adult Indian population was 4.4%, thus the incidence in these pill users was not increased. The significance of the immunologic findings was unknown.
 ...
PMID:Immunological status of women with prolonged oral contraceptives and occurrence of giardiasis. 228 71

Sixty children with chronic diarrhoea, age ranging from 9 months to 3 years and 15 normal healthy children of same age group, all belonging to the low socio-economic families formed the basis of this study. Fifty-six out of these 60 children were undernourished and were marasmic. Stool examination showed enteropathogenic E. coli in 24 (40 per cent), Ascaria lumbricoides in 12 (20 per cent) and Giardia lamblia in 6 (10 per cent). Coeliac disease was detected in 2 (3 per cent) and combined IgA-IgG deficiencies were found in one case (2 per cent). No cause could be found in 15 (25 per cent) cases. Multiple aetiological factors were found in 7 (12 per cent) cases. Stool IgA levels were significantly elevated in the patients than in the controls and more so in the patients with giardiasis and also in patients with coeliac disease. Serum IgA levels were remarkably raised in the patients with diarrhoea due to enteropathogenic E. coli, indicating probable spilling of gut-associated IgA into the circulation. No IgA was detected in the stool of a dysgammaglobulimic patient, who had both serum IgA and IgG deficiencies.
 ...
PMID:Raised stool and serum IgA levels in undernourished infants with chronic diarrhoea and associated parasitic infestations. 235 7

120 patients with Giardia lamblia infection diagnosed by biliary drainage or jejunal mucosa smear are studied. 80 patients showed frequent reinfections by the parasites (recurrent giardiasis). A study is made of T and B lymphoid populations in peripheral blood, which were reduced in all patients with giardiasis in relation with the control group (p less than 0.01). Changes were more pronounced in relapsing than in non-relapsing patients. Retarded in vivo hypersensitivity tests with memory and recognizing antigens did not show significant differences between the experimental and control group. Serum levels of IgG, IgM, and IgA differed (p less than 0.01) between patients and controls at the expense of an IgA increase and IgG and IgM decrease, although these variations were not significant between relapsing and non-relapsing patients. It is concluded that the immunity status of the host is of interest in the clinical picture of patients with giardiasis and therefore must be a factor to be considered in the study of Giardia lamblia infected patients.
 ...
PMID:[Giardiasis. Aspects of humoral and cellular immunity]. 248 21

Parasitosis opportunist are becoming clearer thanks to a better knowledge of immunological mechanisms, especially in AIDS. Child immunological immaturity and corticotherapy are the two other main immunodeficiencies among opportunist parasitosis. For the protozoosis, coccidiosis (especially toxoplasmosis), cryptosporidiosis, but isosporosis too and microsporidiosis represent a privileged group among opportunistic infections. Among adult, leishmaniasis caused by L. infantum is an opportunist parasistosis, favoured by corticotherapy or AIDS, but among children, it is the child's immunological immaturity which is involved in the immunodeficiency. Babesia occurs among splenectomized people. Giardiasis is more frequent and more severe among IgA immunodeficiencies especially secretories IgA. Among helminthiasis, generalised strongyloidiasis is very severe among patients under corticotherapy, but AIDS is not involved.
 ...
PMID:[Opportunistic aspects of parasitosis]. 268 97

During an outbreak of diarrheal illness among residents of a trailer park in rural Vermont, 37 (30%) of 122 residents met the case definition of outbreak-related giardiasis. Convalescent-phase sera from 24 residents and 20 nonresident control subjects were tested by enzyme-linked immunosorbent assay for immunoglobulin G (IgG), IgM, and IgA antibodies to Giardia lamblia. Residents showed higher levels of parasite-specific antibody than did nonresident controls for IgG and IgA but not IgM. Nine residents with giardiasis had a higher median level of G. lamblia-specific IgA but not IgG or IgM than 15 healthy residents (0.61 versus 0.16 optical density units; P = 0.004). Moreover, parasite-specific IgA levels were higher in those consuming tap water than in those who did not (0.31 versus 0.08 optical density units; P = 0.03) and increased with increasing water consumption. Levels of serum antibody to G. lamblia, particularly IgA, may be useful in determining exposure to G. lamblia-contaminated water and illness from G. lamblia during waterborne outbreaks of diarrheal illness.
 ...
PMID:Elevated levels of immunoglobulin A to Giardia lamblia during a waterborne outbreak of gastroenteritis. 276 60

Following Giardia lamblia infection in immune intact NMRI mice, increased numbers of IgM-containing cells and decreased numbers of IgA containing cells were noticed in the lamina propria during the establishment (3-5 days) and acute (9-11 days) phases of infection. The decline in IgM-containing cells during the clearance phase of infection (17-21 days post-infection) was accompanied by an increase in IgA and IgG-containing cells. Our data suggest that the locally synthesized antibodies, especially of the IgA class, play a significant immunodulatory role in the clearance of G. lamblia infection from the gut. Mice immunosuppressed using rabbit anti-mouse lymphocyte serum or dexamethasone had significantly reduced numbers of IgA and IgG-containing cells during all phases of infection and higher parasite loads in their jejunum. It appears that one of the reasons for increased severity and chronicity of G. lamblia infection is the decrease in immunoglobulin-containing cells in the gut.
 ...
PMID:Sequential changes of lamina propria immunoglobulin-containing cells in immune intact and immunosuppressed mice infected with Giardia lamblia. 279 5

Cryptosporidiosis, giardiasis, trichomoniasis, and distemper were diagnosed in a 6-month-old female Siberian Husky pup. Poor growth rate, mucopurulent ocular and nasal discharges, and diarrhea were observed. Results of immunologic studies revealed decreased serum IgG concentration and undetectable serum IgA concentration. Cultured lymphocytes yielded a less-than-adequate response to mitogen stimulation. The serum also contained a factor that suppressed mitogen stimulation in control cultured lymphocytes.
 ...
PMID:Cryptosporidiosis associated with immunosuppression attributable to distemper in a pup. 325 52


<< Previous 1 2 3 4 5 6 7 8 Next >>