Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0017536 (giardiasis)
 1,714 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An outbreak of giardiasis was observed in a sheep farm in Central Italy. Infected lambs (30-90 days of age) showed a malabsorption syndrome, decreased weight gain and impairment in feed efficiency. The most relevant clinical sign was the excretion of malodorous and poorly formed faeces, whereas diarrhoea was rarely observed in the flock. Laboratory investigations revealed the presence of Giardia in affected animals, while no other significant viral, bacterial or parasitic pathogens were identified in faeces or tissue samples. A mild to severe infiltrative enteritis with eosinophils, lymphocytes and plasma cells was detected in histological sections of the gut. Giardia parasites collected from duodenal aspirates were typed as Giardia duodenalis Assemblage B, by PCR amplification and sequencing of the TPI gene. Treatment with fenbendazole at a dose of 10mg/kg for 3 consecutive days, successfully cleared the infection. These results show that G. duodenalis can cause significant economic losses in sheep farming.
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PMID:Severe weight loss in lambs infected with Giardia duodenalis assemblage B. 1689 Oct 57

Giardia duodenalis is a pathogen that has been found in a variety of mammals, including humans, and consists of host-specific (assemblages C, D, E, F, and G) and zoonotic (assemblages A, B) genetic groups. Ferrets are popular pets, and they, like humans, are hosts to this pathogen. The genetic characteristics of the Giardia population in ferrets are unclear because only one ferret isolate has been genotyped. To develop a more complete picture of the genetic characteristics of the Giardia population in domestic ferrets two additional Giardia isolates, recovered independently from two ferrets suffering from intestinal or hepatic giardiasis, were analyzed genetically. The sequences of both isolates at three loci, small subunit ribosomal RNA (SSUrDNA, 292 bp), beta-giardin (734 bp), and glutamate dehydrogenase (GDH, 713 bp) were identical to each other, but the sequences of triosephosphate isomerase locus (TPI, 512 bp) had five substitutions between isolates. Sequence homology and phylogenetic analyses at four loci identified both isolates as members of the assemblage A. Moreover, the sequences of SSUrDNA, beta-giardin, and GDH from both isolates were identical to those of the previous ferret isolate genotyped as assemblage A within the regions of overlap. The result obtained in the present study indicates that at least two genetically different types in assemblage A exist in domestic ferrets. In addition, there have been no reported human and animal isolates with the same sequence as those from ferret isolates at all four loci examined, suggesting that the present ferret isolates might be host-specific.
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PMID:Molecular characterization of Giardia duodenalis isolates from domestic ferrets. 2005 61

An infection of digestive system, Giardiasis, caused by tiny parasites called Giardia lamblia (also known Giardia intestinalis or Giardia duodenalis). Giardia sp. is the most common intestinal parasite of humans and other animals throughout the world. Isolates of G. lamblia are classified into eight assemblages based on isoenzyme and DNA analyses. Assemblages A and B infect humans and a broad range of other hosts. The purpose of this study was to genotype human isolates of G. lamblia by PCR-RFLP in Karaj city. 60 positive fecal samples of G. lamblia were collected. DNA extraction and amplification of TPI gene were successfully conducted by nested-PCR. Subsequently, all samples were positive. Sequencing on 5 samples was conducted to determine genetic differences. The presence of 2 genotypes of G. lamblia (A and B) was revealed by the alignment of the TPI sequences obtained with reference sequences. The results of RFLP technique show that 35 of 60 (58.3%) isolates belonged to assemblage A, and 17 of 60 (28.3%) belonged to assemblage B but 1(1.7%) sample was not determined. Whereas, 7 (11.6%) specimens were detected as mixed infections. The latter RFLP was carried out to identify subtypes.The final results were 100% (35/35) AII, 82.3% (14/17) BIII, and 17.7% (3/17) BIV. This study suggests that the modified RFLP method is favorably time saving and easily achievable and highly economical. Hence, the sub-assemblage AII might be dominant in Karaj city.
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PMID:A modified PCR-RFLP method to determine genetic diversity of Giardia lamblia human isolates based on triosephosphate isomerase (TPI) gene. 2990 69