Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0017536 (giardiasis)
 1,714 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The mechanism of mucosal injury in Giardia lamblia-infected animals and humans is not well understood, although the role of gut macrophages in killing the trophozoites is well known. It is speculated, however, that macrophage products have a role in tissue injury and inflammatory response during infection, as in other inflammatory diseases. Therefore, in the present study an attempt was made to examine the mechanism involved in enterocyte damage during giardiasis. This was achieved using co-culture of enterocytes and gut macrophages obtained from infected BALB/c mice. The extent of tissue damage was assessed by measuring the marker enzyme of enterocyte damage, lactate dehydrogenase. To investigate the role of the various proteases and free oxygen radicals released by activated macrophages on enterocyte damage, inhibitors of various proteases and free oxygen radicals were used. Superoxide radical and certain proteases were found to have important roles in bringing about enterocyte damage during this infection in mice. Parasite load, lactate dehydrogenase release, and extent of lipid peroxidation were more pronounced in mice infected with symptomatic strains than in asymptomatic ones. The theory of inflammatory cell-mediated enterocyte damage in Giardia lamblia infection is proposed.
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PMID:Macrophage-mediated enterocyte damage in BALB/c mice infected with different strains of Giardia lamblia. 823 42

The effect of antioxidant (Antox) on Giardia lamblia and Microsporidium sp. in rats and mice respectively was studied. Parasitologic effect was assessed by the mean parasitic count in infected animals' stool treated and non-treated, and infection intensity in stained section. Biochemical by measuring activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO) levels and cytokine induced neutrophil chemoattractant-1 (CINC-1) in intestinal homogenates in these animals as shown by cell injury, lipid peroxidation and neutrophil infiltrations. The present results showed that Antox significantly exacerbated G. lamblia and Microsporidium sp. This was manifested by a significant increase in number of G. lamblia cysts and trophozoites in stool and intestinal sections of treated infected rats. Also, microsporidian spores were significantly higher in stool of treated infected mice and infection intensity increased in the intestinal sections. The biochemical study showed a significantly higher degree of cell injury, lipid peroxidation and intestinal neutrophils accumulation in non-treated infected animals whether with G. lamblia or microsporidia. The changes reduced after treatment in giardiasis but none in microsporidiosis. The results were tabulated photographed, and critically discussed.
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PMID:Influence of the antioxidant drug (Antox) on experimental giardiasis and microsporidiosis. 1758 May 77