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Query: UMLS:C0017536 (giardiasis)
 1,714 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Giardia has been frequently implicated as a causative agent for acute as well as chronic diarrheal diseases in children. The present study was aimed at exploring the determinants of manifestations of Giardiasis in childhood, in relation to various host and parasite related factors. A total of 200 children with acute (100), chronic (50) or without (50) diarrhea in last 15 days were recruited for the study and evaluated with regards to nutritional status, serum immunoglobulins, secretory IgA levels, presence of Giardia in stool/duodenal aspirate/duodenal biopsy specimen and for associated infections. Lysates from acute giardiasis cases were further studied for zymodeme (banding) pattern. After correlation of all investigations, humoral immune defect in the host was found to be the major determinant of whether the Giardial infestation would be symptomatic or not, while associated bacterial infections and zymodeme patterns were not found to be important in determining the pathogenicity or presentation of giardiasis.
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PMID:Determinants of symptomatic giardiasis in childhood. 878 80

Giardiasis and cryptosporidiosis are diseases caused by the protozoan parasites Giardia lamblia and Cryptosporidium parvum. Waterborne transmission of these organisms has become more prevalent in recent years, and regulatory agencies are urging that source and finished water be screened for these organisms. A major problem associated with testing for these organisms is the lack of reliable methodologies and baseline information on the prevalence of these parasites in various water sources. Our study addressed both of these issues. We evaluated the presence and reduction of Giardia cysts and Cryptosporidium oocysts in sewage effluent by a combination of indirect fluorescent antibody (IFA) staining and PCR. Our results indicated a 3-log reduction of Giardia cysts and a 2-log reduction of Cryptosporidium oocysts through the sewage treatment process as determined by IFA. We developed a nested PCR to detect Cryptosporidium oocysts and used a double PCR to detect Giardia cysts. A 100% correlation was noted between IFA and PCR detection of Giardia cysts while correlation for Cryptosporidium oocysts was slightly less. On the basis of these results, PCR may be a useful tool in the environmental analysis of water samples for Giardia and Cryptosporidium organisms.
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PMID:Evaluation of PCR, nested PCR, and fluorescent antibodies for detection of Giardia and Cryptosporidium species in wastewater. 878 6

Five studies were carried out in children younger than 4 years old attending regularly day care centres in order to determine the intestinal parasitization by Cryptosporidium and Giardia. The percent of children parasitized by Cryptosporidium was 10% (17 children out of 170 studied). Cryptosporidiosis was more frequent in winter than in other seasons (difference statistically significant). Giardia intestinalis, which was the most frequent parasite, was identified in 25.3% (43 children out of 170 studied). Giardiasis was more frequent in autumn and in the low SEL (difference statistically significant in both cases). The frequency of giardiasis increased from 8% in children studied during 1-2 seasons, to 34% in children studied for 3-5 seasons (difference statistically significant), whereas the frequency of cryptosporidiosis did not show variation with increased number of specimens studied per child. The 35% of children parasitized by Cryptosporidium sp. showed gastrointestinal symptoms but only 14% of children with Giardia intestinalis.
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PMID:Seasonal prevalences of Cryptosporidium and Giardia infections in children attending day care centres in Salamanca (Spain) studied for a period of 15 months. 888 97

Giardiasis is a cosmopolitan parasitic disease. About 10% of adults and 20% of children in Poland is infected with Giardia lamblia but only a few per cent of cases is symptomatic. Incidence and symptomatology depend upon a number of factors including individual susceptibility. The disease is usually chronic and recurrent and acute form is found in rare cases only. Giardiasis should be suspected in patients with chronic atypical symptoms related to the alimentary tract. Nitroimidasolic compounds are used in the treatment of giardiasis.
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PMID:[Giardiasis]. 897 96

An experiment was undertaken to determine the effect of salinomycin on Giardia in vitro and on Giardia and coccidia in growing lambs. Concentrations of salinomycin above (3.9 micrograms/mL) reduced the adherence (index of viability) of Giardia S2 trophozoites by more than 50%. This strain did not develop resistance after repeated exposure to sublethal concentrations of salinomycin. Five of 40 lambs escaped natural infection by Giardia, and these were inoculated with > or = 500,000 cysts. Giardiasis (presence of cysts in feces) was confirmed in all lambs before commencement of the experiment. Coccidiosis (presence of oocysts) developed by natural exposure. Lambs were assigned randomly to diets containing 0, 4, 10, or 16 ppm of salinomycin. Giardia cyst and coccidia oocyst excretions were determined on 6 d during the first week and weekly thereafter. Giardia cysts were detected at each sampling date in all treatments (highest release, 2.3 x 10(6) cysts/g feces). The number of Giardia cysts shed in feces was not affected (P > .05) by salinomycin but did decline (P < .05) with time. Average infection rates remained above 50% until d 41 of the experiment and declined linearly (P < .05) with salinomycin concentration and time. The number of coccidia oocysts in feces was low (highest release, 6.8 x 10(4) oocysts/g feces), but shedding occurred in 38 of the 40 lambs. Treatment with salinomycin had a cubic effect (P < .05) on coccidia oocyst excretion, and no oocysts were detected beyond d 28. Treatment effect on average daily gain (P < .002), dry matter intake (P < .02), and final live weight (P < .07) was cubic, whereas carcass weight (P < .003) and dressing percentage (P < .08) were linearly affected by salinomycin concentration. Although a beneficial effect of 10 ppm salinomycin on lamb performance was apparent, the development of natural resistance makes it difficult to attribute this response to the control of coccidiosis or giardiasis.
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PMID:Effect of salinomycin on giardiasis and coccidiosis in growing lambs. 899 2

The prevalence of the Giardia duodenalis and Cryptosporidium parvum infections were studied in children 2-60 months old (n = 310) grouped to sex and age, enrolled in 8 day-care centers. Giardiasis was found in 42 (13.5%) of the children and, was most frequent in the age group corresponding to 19-24 months old although children at the first six months of life also presented positive faecal samples. Statistical analysis indicated no association between sex (p > 0.05) and prevalence for Giardia infection. C. parvum was detected in 20 (6.4%) of the children. Children seven to twelve months old showed the greatest prevalence of this parasite. There were not oocysts in the stools from the children 25-30 months and from those older than 36 months. Oocytes were detected in 4 children from the 0-6 months old group. There was no significative difference on sex (p = 0.09) and day-care-facility (p = 0.068) for C. parvum infection while age was associated with infection (p = 0.004). Epidemiological aspects were studied by familiar inquiry, by parasitological examinations of stools from the contact-members of each case and from animals present at the residence. Prevalence for C. parvum was higher when at least one of the parents was a professional in the Human Health Centers (73.6% positives), when there was report of concomitant disease (52.6% positives) and when diarrhoea was the main symptom at the collection of the faecal specimens (78.9% positives). Faecal examinations of the domestic animals were negatives for these two parasites.
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PMID:[Giardiasis and cryptosporidiosis in day-care centers in the municipality Campinas SP]. 901 85

A study was undertaken to determine the prevalence of Giardia infections in dairy calves and to compare Giardia and Cryptosporidium infections in calves of different ages. Fresh fecal samples were collected from 386 male and female Holstein calves (newborn to 24 wk) in 20 dairies located in the lower Fraser river valley area of British Columbia. Giardia intestinalis, Cryptosporidium parvum, and Cryptosporidium muris were enumerated in each sample after concentration by sucrose gradient centrifugation and immunofluorescent staining. Giardia was identified at all farm locations. The overall prevalence of Giardia in calves was 73% with a geometric mean cyst count of 1180 cysts per gram of feces (CI, 41 to 5014). Cryptosporidium parvum and C. muris were identified in 80% and 40% of the farms, respectively. The prevalence of C. parvum was 59%, and the geometric mean for oocysts was 457 oocysts per gram of feces (CI, 18 to 160). The prevalence of C. muris was only 2% and the mean oocyst counts were 54 oocysts per gram of feces. Giardiasis was not age dependent, and approximately 80% of the calves from 2 to 24 wk were infected. In contrast, C. parvum infections were predominant in calves 2 to 4 wk, while C. muris was demonstrated in calves older than 4 wk. Fourty-seven percent of calves with diarrhea had high numbers of Giardia cysts in their feces. Giardia infections are highly prevalent in dairy calves and should be considered in animals with diarrhea or failure to thrive.
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PMID:Giardia and Cryptosporidium in dairy calves in British Columbia. 936 Jul 89

Giardiasis has been associated with an increase in allergic disease following infection suggesting an alteration in mucosal immune function. Jejunal in vivo and in vitro macromolecular transport, epithelial permeability, and mucosal and connective tissue mast cell counts were examined in Mongolian gerbils (35-45 g) orogastrically inoculated (I) with a pathogenic strain of Giardia lamblia and compared to age- and weight-matched, sham-treated controls (C) 6 and 21 days postinoculation. Macromolecular uptake was significantly increased in infected tissue at 6 days both in vivo (I 134 +/- 19 vs. C 74 +/- 17 ng/hr; n = 8; P < 0.05) and in vitro (I 125 +/- 17 vs. C 67 +/- 8 ng/hr/cm; n = 12; P < 0.05). Macromolecular uptake did not differ between groups at 21 days. Infection had no effect on mucosal permeability of [51Cr]EDTA. Mucosal mast cell counts did not differ at 6 days but were significantly elevated in infected tissue at 21 days (I 33.3 +/- 6.8 vs. C 2.7 +/- 0.4 per high magnification field; n = 5; P < 0.01) as were connective tissue mast cell counts (I 1.7 +/- 0.2 vs. C 1.0 +/- 0.1 per high magnification field; n = 13; P < 0.005). The findings indicate that during the peak phase of giardiasis, jejunal active antigen uptake is increased leading to a delayed recruitment of mucosal and connective tissue mast cells. These changes may play a role in the increased incidence of hypersensitivity reactions associated with Giardia infection.
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PMID:Mast cell hyperplasia and increased macromolecular uptake in an animal model of giardiasis. 937 97

We worked with 185 middle-class patients above 18 years of age, both sexes, who presented diarrhea and/or chronic gastrointestinal disorders. The faeces were collected serially in formol 10% and processed in the following way: direct microscopy, with and without wet staining, concentration by Ritchie's method, 1% safranine technique for a specific investigation of Cryptosporidium sp., and faecal sieving macroparasites. Twenty eight point six of the studied patients showed at least one enteroparasite in their faeces, 48 harboured one parasite and 5 harboured two parasites. The following parasites were found and their corresponding percentages in the entire studied population are given below: Blastocystis hominis 15.7%, Giardia lamblia 7.5%, Cryptosporidium sp. 1.6%, Entamoeba coli 3.3%, Chilomastix mesnilii 1.1%, Ancylostoma duodenale-Necator americanus 0.5%, Ascaris lumbricoides 0.5%, Enterobious vermicularis 0.5% y Endolimax nana 0.5%. The most frequently found enteroparasites in the positive patients were B. hominis and G. lamblia. Cryptosporidium sp. was diagnosed in only three patients. The source of infection could be presumed in all of them. The symptomatology coincided with that described for this coccid in the bibliography. In spite of the fact that they were HIV seronegative patients the diarrhea was not self-limiting, but the immunologic profile of their relatives remained unknown and no other cause of immunosuppression could be detected with justified chronicity. The treatment with spiramycin was effective. Giardiasis was found in 17 patients, and the source of infection could not be inferred in any of them. They all had chronic diarrhea and their most frequent symptoms were abdominal pain, metallic taste, flatulency and nausea. Most of these patients were harboured one parasite, and only 2 of them simultaneously presented another faecal parasite associated to G. lamblia. Treatment with metronidazole was successful in all of them. Twenty nine patients were found to have B. hominis. The source of infection could not be inferred, this amoeboid was present as the only parasite in 25 patients. Predominant symptoms were flatulence, abdominal distention and colis. All patients suffered from chronic diarrhea, alternating, in some cases, with constipation. Good therapeutic results were obtained with metronidazole. Considering that one third of the patients examined presented faecal parasites associated to chronic disorders, it is important to insist on the detection of parasites to chronic disorders, it is important to insist on the detection of parasites using appropriate diagnostic techniques since the application of specific therapy made their eradication possible as well as relieving the patients' symptomatology.
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PMID:[Parasitosis in an adult population with chronic gastrointestinal disorders]. 941 36

Giardia lamblia, a gastrointestinal protozoan, is one of the most common disease-causing parasites in the world. Giardiasis is primarily encountered in areas with poor sanitation, but it is also seen in more developed countries. A possible sequela of Giardia infections of the bowel is reactive arthritis or synovitis. Few reports of synovitis secondary to giardiasis exist in the literature. Arthropathy secondary to giardiasis is uncommon, but may be underdiagnosed. In this study, Giardia synovitis was diagnosed in two children at a major children's hospital over a 20-year period. Both were boys, aged 7 years, 6 months and 1 year, 8 months at the time of presentation. The knee was the affected joint in both patients, and both cases were initially misdiagnosed as septic arthritis. The synovitis subsided with treatment of the giardiasis, one with cefuroxime and the other with cefuroxime and metronidazole. The diagnosis of Giardia synovitis should be suspected by the presence of Giardia cysts in the stool, similar symptoms in other family members, a synovial white count under 40,000, and an increase in the eosinophil count. Although uncommon, giardiasis can cause severe synovitis that may be confused with a septic joint.
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PMID:Synovitis secondary to giardiasis in children. 965 90


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